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Team:Chiba/Project
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Project overview
E.coli time manager
We control the timing of gene expression by using multiple signaling devices.To this end,we utilize molecules associated with Quorum sensing, a phenomenon that allows bacteria to communicate with each other.Our project uses two classes of bacteria: senders and receivers. Senders produce signaling molecules, and Receivers are activated only after a particular concentration of this molecule is reached.Although different quorum sensing species have slightly different signaling molecules, these molecules are not completely specific to their hosts and cross-species reactivity is observed.Communication using non-endogenous molecules is less sensitive, andrequires a higher signal concentration to take effect.This results in slower activation of receivers.
Project Design
About Quorum Sensing
Quorum sensing is a cell-to-cell signaling action of bacteria. They detect the cell density of the same species and coordinate the expression behavior of their cells. Species of Gram-Negative signaling transfer molecules (so-called autoinducer) is a series of acyl homoserine lactone (AHL). When the AHL concentration exceeds a threshold, that is, the cell population is over a certain number, the promoter plux transcription is activated.
Controlling the Delay time of the AHL signaling
- Quorum-Sensing Cross-talk
- LuxR/Plux mutant
Experiments
AHL signaling test
Results
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