Team:Chiba/Sender experiments
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#Inoculated them independently in liquid media. Incubated at 37°C 12h | #Inoculated them independently in liquid media. Incubated at 37°C 12h | ||
#Mixed them. | #Mixed them. | ||
- | #Incubated at | + | #Incubated at 30°C. |
#Measured intensity of green fluorescence at regular time intervals. | #Measured intensity of green fluorescence at regular time intervals. | ||
Revision as of 09:41, 25 October 2008
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Project Design ( Sender experiments | Receiver experiments ) | Our Goal |
Contents |
Quorum-Sensing Cross-talk
Purpose
Senders
Receiver
Method
To characterize quorum sensing crosstalk, constitutive AHL senders were mixed with constitutive receivers and measure fluorescence intensity.
- Transformed Senders into E.coli strains(JW1908/XL10GOLD) and Receiver into E.coli strain(JW1908).
- Inoculated them independently in liquid media. Incubated at 37°C 12h
- Mixed them.
- Incubated at 30°C.
- Measured intensity of green fluorescence at regular time intervals.
Result
1.Crosstalk test at 37°C
- LasI
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