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Team:Chiba/Sender experiments
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====1.Crosstalk test==== | ====1.Crosstalk test==== | ||
[[image:senders_crosstalk_chiba_01.gif|frame|left| | [[image:senders_crosstalk_chiba_01.gif|frame|left| | ||
| - | '''Fig.''' senders crosstalk test.senders strain XL10Gold,Receiver strain JW1908.Reaction temparature was 30°C.Labeling:LuxI,RhlI,LasI means fluorescence induced by AHLs synthesized by LuxI,RhlI,LasI respectively.]]<br clear="all"> | + | '''Fig.''' senders crosstalk test.senders strain XL10Gold,Receiver strain JW1908.Reaction temparature was 30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.Labeling:LuxI,RhlI,LasI means fluorescence induced by AHLs synthesized by LuxI,RhlI,LasI respectively.]]<br clear="all"> |
| - | *RhlIとLuxIでは、GFP | + | *RhlIとLuxIでは、GFP inductionにかかる時間はほとんど同じであった. |
| - | *LasIは、GFP | + | |
| + | *LasIは、GFP inductionが他より約2時間遅れた. | ||
| + | |||
| + | |||
| + | （冨永） | ||
====2.Demonstration==== | ====2.Demonstration==== | ||
Revision as of 00:16, 26 October 2008
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Contents |
Quorum-Sensing Cross-talk
Purpose
To Confirm that communication using non-endogenous molecules results in slower activation of receivers.
冨永
Fig.5 Senders crosstalk
Senders
Receiver
Method
To characterize quorum sensing crosstalk, constitutive AHL senders were mixed with constitutive receivers and measure fluorescence intensity.
- Transformed Senders into E.coli strains(JW1908/XL10GOLD) and Receiver into E.coli strain(JW1908).
- Inoculated them independently in liquid media. Incubated at 37°C 12h
- Mixed them.
- Incubated at 30°C.
- Measured intensity of green fluorescence at regular time intervals.
Result
1.Crosstalk test
Fig. senders crosstalk test.senders strain XL10Gold,Receiver strain JW1908.Reaction temparature was 30°C.All measurements are averages from three replicate cultures with error bars representing standard deviations.Labeling:LuxI,RhlI,LasI means fluorescence induced by AHLs synthesized by LuxI,RhlI,LasI respectively.
- RhlIとLuxIでは、GFP inductionにかかる時間はほとんど同じであった.
- LasIは、GFP inductionが他より約2時間遅れた.
（冨永）
2.Demonstration
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