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Revision as of 05:23, 22 September 2008 (view source) Masahiro (Talk | contribs) (→time-delay実験結果) ← Older edit		Revision as of 04:06, 25 September 2008 (view source) Masahiro (Talk | contribs) (→To Do List) Newer edit →
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-	==To Do List==
-	*出力の決定（杉山、福冨）
-	**LacZ-->基質濃度に依存
-	**rLuciferine-->見えない？-->基質が必要、光分解性（詳しく調べる）
-	*Rs,PFL(LuxR,J3720-->imperial,2006)の作成（杉山、福冨）
-	*入力の決定（香取）
-	**Arabinose promotor?-->線形成？On/Offはっきり？
-	**On/Offの必要性は-->なし？
-	*Time-delayの確認（井山、川崎、小林、冨永）
-	**cinIのクローニング（井山、川崎）<Br>
-	**差を広げる-->温度依存性、培地依存性.PFL(Positive feedback loop)(BBa_J37015,BBa_J37020)の利用.
-	**AHL固定(1nM,10nM,100nM~)-->閾値（10nM付近以上）を超えると、GFPによる蛍光強度は変わらない。4~5時間で飽和する。
-	**固体培地にspot
-	**His TagつきGFP(その他タンパク質)を発現-->freeze-->SDS-PAGE-->blotting
-	**Is(BW,XL10G)をT9002(BW)でdelay check
-	***さらに-BBa_J37020を形質転換(XL10G,BW)でtime-delayをチェック
-	*work out deal to print T-shirts (井山、福冨)
-
-	===still under discussion===
-	*出力を交換できる形でクローニングしよう-->intermideatesの作成
-	*AHL synthase のkineticsについての情報は？-->[[Team:Chiba/Reference/j|ref.10 and ref.11]]
-	*ベクターの乗せ換え(PSB1-->P15A)（井山、小林、川崎）<Br>
-
-	===連絡===
-	実験結果より
-	-->Rs濃度が律速→PFL(J37020)使う？Rs替える？
-	----
	{|class=wikitable align=center width=900		{|class=wikitable align=center width=900

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Revision as of 04:06, 25 September 2008

>Notebook

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August M T W T F S S         [http://2008.igem.org/Team:Chiba/Calendar-Main/1_August_2008 1] [http://2008.igem.org/Team:Chiba/Calendar-Main/2_August_2008 2] [http://2008.igem.org/Team:Chiba/Calendar-Main/3_August_2008 3] [http://2008.igem.org/Team:Chiba/Calendar-Main/4_August_2008 4] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/5_August_2008&action=edit 5] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/6_August_2008&action=edit 6] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/7_August_2008&action=edit 7] [http://2008.igem.org/Team:Chiba/Calendar-Main/8_August_2008 8] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/9_August_2008&action=edit 9] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/10_August_2008&action=edit 10] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/11_August_2008&action=edit 11] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/12_August_2008&action=edit 12] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/13_August_2008&action=edit 13] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/14_August_2008&action=edit 14] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/15_August_2008&action=edit 15] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/16_August_2008&action=edit 16] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/17_August_2008&action=edit 17] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/18_August_2008&action=edit 18] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/19_August_2008&action=edit 19] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/20_August_2008&action=edit 20] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/21_August_2008&action=edit 21] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/22_August_2008&action=edit 22] [http://2008.igem.org/Team:Chiba/Calendar-Main/23_August_2008 23] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/24_August_2008&action=edit 24] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/25_August_2008&action=edit 25] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/26_August_2008&action=edit 26] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/27_August_2008&action=edit 27] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/28_August_2008&action=edit 28] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/29_August_2008&action=edit 29] [http://2008.igem.org/Team:Chiba/Calendar-Main/30_August_2008 30] [http://2008.igem.org/Team:Chiba/Calendar-Main/31_August_2008 31]

September M T W T F S S [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/1_September_2008&action=edit 1] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/2_September_2008&action=edit 2] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/3_September_2008&action=edit 3] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/4_September_2008&action=edit 4] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/5_September_2008&action=edit 5] [http://2008.igem.org/Team:Chiba/Calendar-Main/6_September_2008 6] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/7_September_2008&action=edit 7] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/8_September_2008&action=edit 8] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/9_September_2008&action=edit 9] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/10_September_2008&action=edit 10] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/11_September_2008&action=edit 11] [http://2008.igem.org/Team:Chiba/Calendar-Main/12_September_2008 12] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/13_September_2008&action=edit 13] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/14_September_2008&action=edit 14] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/15_September_2008&action=edit 15] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/16_September_2008&action=edit 16] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/17_September_2008&action=edit 17] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/18_September_2008&action=edit 18] [http://2008.igem.org/Team:Chiba/Calendar-Main/19_September_2008 19] [http://2008.igem.org/Team:Chiba/Calendar-Main/20_September_2008 20] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/21_September_2008&action=edit 21] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/22_September_2008&action=edit 22] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/23_September_2008&action=edit 23] [http://2008.igem.org/Team:Chiba/Calendar-Main/24_September_2008 24] [http://2008.igem.org/Team:Chiba/Calendar-Main/25_September_2008 25] [http://2008.igem.org/Team:Chiba/Calendar-Main/26_September_2008 26] [http://2008.igem.org/Team:Chiba/Calendar-Main/27_September_2008 27] [http://2008.igem.org/Team:Chiba/Calendar-Main/28_September_2008 28] [http://2008.igem.org/Team:Chiba/Calendar-Main/29_September_2008 29] [http://2008.igem.org/Team:Chiba/Calendar-Main/30_September_2008 30]

October M T W T F S S     [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/1_October_2008&action=edit 1] [http://2008.igem.org/Team:Chiba/Calendar-Main/2_October_2008 2] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/3_October_2008&action=edit 3] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/4_October_2008&action=edit 4] [http://2008.igem.org/Team:Chiba/Calendar-Main/5_October_2008 5] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/6_October_2008&action=edit 6] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/7_October_2008&action=edit 7] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/8_October_2008&action=edit 8] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/9_October_2008&action=edit 9] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/10_October_2008&action=edit 10] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/11_October_2008&action=edit 11] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/12_October_2008&action=edit 12] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/13_October_2008&action=edit 13] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/14_October_2008&action=edit 14] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/15_October_2008&action=edit 15] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/16_October_2008&action=edit 16] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/17_October_2008&action=edit 17] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/18_October_2008&action=edit 18] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/19_October_2008&action=edit 19] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/20_October_2008&action=edit 20] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/21_October_2008&action=edit 21] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/22_October_2008&action=edit 22] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/23_October_2008&action=edit 23] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/24_October_2008&action=edit 24] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/25_October_2008&action=edit 25] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/26_October_2008&action=edit 26] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/27_October_2008&action=edit 27] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/28_October_2008&action=edit 28] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/29_October_2008&action=edit 29] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/30_October_2008&action=edit 30] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/31_October_2008&action=edit 31]

November M T W T F S S           [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/1_November_2008&action=edit 1] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/2_November_2008&action=edit 2] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/3_November_2008&action=edit 3] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/4_November_2008&action=edit 4] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/5_November_2008&action=edit 5] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/6_November_2008&action=edit 6] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/7_November_2008&action=edit 7] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/8_November_2008&action=edit 8] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/9_November_2008&action=edit 9] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/10_November_2008&action=edit 10] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/11_November_2008&action=edit 11] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/12_November_2008&action=edit 12] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/13_November_2008&action=edit 13] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/14_November_2008&action=edit 14] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/15_November_2008&action=edit 15] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/16_November_2008&action=edit 16] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/17_November_2008&action=edit 17] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/18_November_2008&action=edit 18] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/19_November_2008&action=edit 19] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/20_November_2008&action=edit 20] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/21_November_2008&action=edit 21] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/22_November_2008&action=edit 22] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/23_November_2008&action=edit 23] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/24_November_2008&action=edit 24] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/25_November_2008&action=edit 25] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/26_November_2008&action=edit 26] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/27_November_2008&action=edit 27] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/28_November_2008&action=edit 28] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/29_November_2008&action=edit 29] [http://2008.igem.org/wiki/index.php?title=Team:Chiba/Calendar-Main/30_November_2008&action=edit 30]

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入力班	送受信班	出力班	その他
小林あおい Aoi Kobayashi 香取崇広 Takahiro Katori	冨永将大 Masahiro Tominaga 川崎浩平 Kohei Kawasaki 井山佳美 Yoshimi Iyama	福冨浩樹 Hiroki Fukutomi 杉山まい Mai Sugiyama	Reference Protocols Inventory Ordering

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