Team:Chiba/jk/β

From 2008.igem.org

(Difference between revisions)

Revision as of 13:06, 30 August 2008

β班の実験ノート

Week 1

17 August, 2008

Transforation

BBa_R0040(2008) -> No colonies on plates
BBa_C0070(2008) -> No colonies on plates
BBa_C0076(2008) -> No colonies on plates
BBa_C0077(2008) -> No colonies on plates
BBa_C0078(2008) -> No colonies on plates
BBa_C0079(2008) -> No colonies on plates

18 August, 2008

Transformation

BBa_C0070(2007)
BBa_C0070(2006)
BBa_C0076(2007)
BBa_C0076(2006) -> No colonies on plates
BBa_C0078(2007)
BBa_C0078(2006)
BBa_C0170(2007)
BBa_C0170(2006)
BBa_C0178(2007)
BBa_C0178(2006)

19 August, 2008

PCR

①BBa_I9026(2007)

②BBa_I9026(2006)

③BBa_I9030(2007)

④BBa_I9030(2006)

⑤BBa_S3154(2007)

⑥BBa_S3154(2006)

⑦BBa_F2620(2007)･･･control

Gel Check

①～②

20 August, 2008

Sigma prep

BBa_C0070(2007)
BBa_C0070(2006)
BBa_C0076(2006)
BBa_C0078(2007)
BBa_C0078(2006)

Digestion

BBa_C0170(2007)
BBa_C0170(2006)
BBa_C0178(2007)
BBa_C0178(2006)

Gel Check

21 August, 2008

PCR

BBa_C0070(2007)
BBa_C0070(2006)
BBa_C0076(2006)
BBa_C0078(2007)
BBa_C0078(2006)

Week 2

27 August, 2008

Colony PCR of 5 colonies from ligation plates (27/8) and one from control plate(F2620).

Week 3

reference

L. Steindler.V.Venturi.:Detection of quorum sensing N-acyl homoserine lactone.FEMS Microbiol Lett 266 (2007) 1-9

クオラムセンシングのクロストークについてのミニレビュー。クオラムセンシングを利用したAHLバイオセンサーについて、Table 1.にまとまっている。

M.K Winson et al./FEMS Microbiology Letters 163 (1998) 185-192

Figure 2.hは、LuxR、RhlR、LasRそれぞれの、C4-C14のN-acyl-L-homoserine lactone,C4-C14のN-(3-oxoacyl)-L-homoserine lactoneに対する感受性を比較したグラフ。

ホーム	メンバー紹介	プロジェクト紹介	Parts Submitted to the Registry	モデリング	ノート

@@ Line 29: / Line 29: @@
 *19 August, 2008
 :[[Team:Chiba/protocol/PCR|PCR]]
-::①BBa_I9026(2007)
+::①[http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2007)
-::②BBa_I9026(2006)
+::②[http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2006)
-::③BBa_I9030(2007)
+::③[http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2007)
-::④BBa_I9030(2006)
+::④[http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2006)
-::⑤BBa_S03154(2007)
+::⑤[http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2007)
-::⑥BBa_S03154(2006)
+::⑥[http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2006)
-::⑦BBa_F2620(2007)
+::⑦[http://partsregistry.org/Part:BBa_F2620| BBa_F2620](2007)･･･control
 :[[Team:Chiba/protocol/gelcheck|Gel Check]]