Team:Chiba/jk/β

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*27 August, 2008
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:Colony PCR of 5 colonies from ligation plates (27/8) and one from control plate(F2620).
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:Colony PCR of 5 colonies from ligation plates (27/8) and one from control plate([http://partsregistry.org/Part:BBa_F2620| BBa_F2620](2007)).
==Week 3==
==Week 3==

Revision as of 13:11, 30 August 2008

β班の実験ノート


Contents

Week 1

  • 17 August, 2008
Transforation


  • 18 August, 2008
Transformation
  • 19 August, 2008
PCR
①BBa_I9026(2007)
②BBa_I9026(2006)
③BBa_I9030(2007)
④BBa_I9030(2006)
⑤BBa_S3154(2007)
⑥BBa_S3154(2006)
⑦BBa_F2620(2007)･･･control
Gel Check
①～②
  • 20 August, 2008
Sigma prep
Digestion
Gel Check
  • 21 August, 2008
PCR

Week 2

  • 27 August, 2008
Colony PCR of 5 colonies from ligation plates (27/8) and one from control plate(BBa_F2620(2007)).

Week 3

reference

  • L. Steindler.V.Venturi.:Detection of quorum sensing N-acyl homoserine lactone.FEMS Microbiol Lett 266 (2007) 1-9
クオラムセンシングのクロストークについてのミニレビュー。クオラムセンシングを利用したAHLバイオセンサーについて、Table 1.にまとまっている。
  • M.K Winson et al./FEMS Microbiology Letters 163 (1998) 185-192
Figure 2.hは、LuxR、RhlR、LasRそれぞれの、C4-C14のN-acyl-L-homoserine lactone,C4-C14のN-(3-oxoacyl)-L-homoserine lactoneに対する感受性を比較したグラフ。


ホーム メンバー紹介 プロジェクト紹介 Parts Submitted to the Registry モデリング ノート
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