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From 2008.igem.org

Revision as of 15:31, 30 August 2008 (view source) Yoshimi (Talk | contribs) ← Older edit		Revision as of 15:45, 30 August 2008 (view source) Yoshimi (Talk | contribs) Newer edit →
Line 28:		Line 28:
-	::--->(20/8)[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]	+	:--->(20/8)[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]
Line 43:		Line 43:
-	::--->[[Team:Chiba/protocol/gelcheck|Gel Check]]	+	:--->[[Team:Chiba/protocol/gelcheck|Gel Check]]
	::*[http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2007)		::*[http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2007)
	::*[http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2006)		::*[http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2006)
Line 93:		Line 93:
	::*[http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006)		::*[http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006)
-	:[[Team:Chiba/protocol/gelcheck|Gel Check]]	+
		+	:--->[[Team:Chiba/protocol/gelcheck|Gel Check]]
	::*[http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007) -> OK		::*[http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007) -> OK
	::*[http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006) -> OK		::*[http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006) -> OK
Line 99:		Line 100:
	::*[http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007) -> None		::*[http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007) -> None
	::*[http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006) -> None		::*[http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006) -> None
		+
	:[[Team:Chiba/protocol/transformation|Transformation]]		:[[Team:Chiba/protocol/transformation|Transformation]]
Line 107:		Line 109:
	::*[http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2007)		::*[http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2007)
	::*[http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2006)		::*[http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2006)
		+
		+
	*22 August, 2008		*22 August, 2008
Line 118:		Line 122:
	::*[http://partsregistry.org/Part:BBa_C0077| BBa_C0077](2008) -> No colonies on plates		::*[http://partsregistry.org/Part:BBa_C0077| BBa_C0077](2008) -> No colonies on plates
	::*[http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2008) -> No colonies on plates		::*[http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2008) -> No colonies on plates
		+
		+
	*23 August, 2008		*23 August, 2008
-	:[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]	+	:--->[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]
	::*[http://partsregistry.org/Part:BBa_S03154| BBa_S03154](2007)		::*[http://partsregistry.org/Part:BBa_S03154| BBa_S03154](2007)
	::*[http://partsregistry.org/Part:BBa_S03154| BBa_S03154](2006)		::*[http://partsregistry.org/Part:BBa_S03154| BBa_S03154](2006)
Line 128:		Line 134:
	::*[http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2006)		::*[http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2006)
-	:[[Team:Chiba/protocol/digestion|Digestion]]	+
		+	:--->[[Team:Chiba/protocol/digestion|Digestion]]
	::*[http://partsregistry.org/Part:BBa_S03154| BBa_S03154](2007)		::*[http://partsregistry.org/Part:BBa_S03154| BBa_S03154](2007)
	::*[http://partsregistry.org/Part:BBa_S03154| BBa_S03154](2006)		::*[http://partsregistry.org/Part:BBa_S03154| BBa_S03154](2006)
Line 135:		Line 142:
	::*[http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2007)		::*[http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2007)
	::*[http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2006)		::*[http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2006)
		+	::*[http://partsregistry.org/Part:BBa_J04500| BBa_J04500](2007)
-	:[[Team:Chiba/protocol/gelcheck|Gel Check]]	+
		+	:--->[[Team:Chiba/protocol/gelcheck|Gel Check]]
	::*[http://partsregistry.org/Part:BBa_S03154| BBa_S03154](2007)		::*[http://partsregistry.org/Part:BBa_S03154| BBa_S03154](2007)
	::*[http://partsregistry.org/Part:BBa_S03154| BBa_S03154](2006)		::*[http://partsregistry.org/Part:BBa_S03154| BBa_S03154](2006)

---

Revision as of 15:45, 30 August 2008

β班の実験ノート

Contents 1 Week 1 2 Week 2 3 Week 3 4 Week 4

Week 1

• 17 August, 2008

Transformation

• BBa_R0040(2008) -> No colonies on plates
• BBa_C0070(2008) -> No colonies on plates
• BBa_C0076(2008) -> No colonies on plates
• BBa_C0077(2008) -> No colonies on plates
• BBa_C0078(2008) -> No colonies on plates
• BBa_C0079(2008) -> No colonies on plates

• 18 August, 2008

Transformation

• BBa_C0070(2007)
• BBa_C0070(2006)
• BBa_C0076(2007)
• BBa_C0076(2006) -> No colonies on plates
• BBa_C0078(2007)
• BBa_C0078(2006)
• BBa_C0170(2007)
• BBa_C0170(2006)
• BBa_C0178(2007)
• BBa_C0178(2006)

--->(20/8)Mini prep

• 19 August, 2008

PCR

• BBa_I9026(2007)
• BBa_I9026(2006)
• BBa_I9030(2007)
• BBa_I9030(2006)
• BBa_S3154(2007)
• BBa_S3154(2006)
• BBa_F2620(2007)･･･control

--->Gel Check

• BBa_I9026(2007)
• BBa_I9026(2006)
• BBa_I9030(2007)
• BBa_I9030(2006)
• BBa_S3154(2007)
• BBa_S3154(2006)
• BBa_F2620(2007)･･･control

• 20 August, 2008

(18/8)--->Mini prep

• BBa_C0070(2007)
• BBa_C0070(2006)
• BBa_C0076(2007)
• BBa_C0078(2007)
• BBa_C0078(2006)
• BBa_C0170(2007)
• BBa_C0170(2006)
• BBa_C0178(2007)
• BBa_C0178(2006)

--->Gel Check

• BBa_C0070(2007) -> OK
• BBa_C0070(2006) -> OK
• BBa_C0076(2007) -> None
• BBa_C0078(2007) -> None
• BBa_C0078(2006) -> OK
• BBa_C0170(2007) -> OK
• BBa_C0170(2006) -> OK
• BBa_C0178(2007) -> OK
• BBa_C0178(2006) -> OK

--->Digestion

• BBa_C0170(2007)
• BBa_C0170(2006)
• BBa_C0178(2007)
• BBa_C0178(2006)

• 21 August, 2008

PCR

• BBa_C0070(2007)
• BBa_C0070(2006)
• BBa_C0076(2007)
• BBa_C0078(2007)
• BBa_C0078(2006)

--->Gel Check

• BBa_C0070(2007) -> OK
• BBa_C0070(2006) -> OK
• BBa_C0076(2007) -> None
• BBa_C0078(2007) -> None
• BBa_C0078(2006) -> None

Transformation

• BBa_S03154(2007)
• BBa_S03154(2006)
• BBa_I9026(2007)
• BBa_I9026(2006)
• BBa_I9030(2007)
• BBa_I9030(2006)

• 22 August, 2008

Transformation

• BBa_J13002(2007)
• BBa_J13002(2006)
• BBa_J04500(2007)
• BBa_J04500(2006)

(Locate & 2 Punch ~iyama ver.~)

• BBa_C0076(2008) -> No colonies on plates
• BBa_C0077(2008) -> No colonies on plates
• BBa_C0078(2008) -> No colonies on plates

• 23 August, 2008

--->Mini prep

• BBa_S03154(2007)
• BBa_S03154(2006)
• BBa_I9026(2007)
• BBa_I9026(2006)
• BBa_I9030(2007)
• BBa_I9030(2006)

--->Digestion

• BBa_S03154(2007)
• BBa_S03154(2006)
• BBa_I9026(2007)
• BBa_I9026(2006)
• BBa_I9030(2007)
• BBa_I9030(2006)
• BBa_J04500(2007)

--->Gel Check

• BBa_S03154(2007)
• BBa_S03154(2006)
• BBa_I9026(2007)
• BBa_I9026(2006)
• BBa_I9030(2007)
• BBa_I9030(2006)

Week 2

• 24 August, 2008

Mini prep

• BBa_J13002(2007)
• BBa_J13002(2006)
• BBa_J04500(2007)
• BBa_J04500(2006)

• 25 August, 2008

• 26 August, 2008

• 27 August, 2008

PCR

Colony PCR of 5 colonies from ligation plates (27/8) and one from control plate(BBa_F2620(2007)).

• 30 August, 2008

Week 3

• 31 August, 2008

• 1 September, 2008

Week 4

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