Team:Chiba/jk/β
From 2008.igem.org
(Difference between revisions)
Line 298: | Line 298: | ||
| | | | ||
From the left; | From the left; | ||
- | :: | + | ::⑤BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007) |
- | :: | + | ::⑥BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2006) |
- | :: | + | ::⑦BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2007) |
- | :: | + | ::⑧BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2006) |
(↓single digestuion) | (↓single digestuion) | ||
- | :: | + | ::⑤BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007) |
- | ::⑥BBa_J04500[http://partsregistry.org/Part:BBa_J04500]( | + | ::⑥BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2006) |
- | :: | + | ::⑦BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2007) |
::⑧BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2006) | ::⑧BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2006) | ||
|} | |} | ||
Line 335: | Line 335: | ||
From the left; | From the left; | ||
(single digestuion) | (single digestuion) | ||
- | :: | + | ::⑤BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007) |
- | ::⑥BBa_J04500[http://partsregistry.org/Part:BBa_J04500]( | + | ::⑥BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2006) |
- | :: | + | ::⑦BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2007) |
::⑧BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2006) | ::⑧BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2006) | ||
|} | |} | ||
Line 343: | Line 343: | ||
*25 August, 2008 | *25 August, 2008 | ||
:(24/8)--->[[Team:Chiba/protocol/digestion|Digestion]] | :(24/8)--->[[Team:Chiba/protocol/digestion|Digestion]] | ||
- | :: | + | ::①Double Digestion:BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007) |
- | :: | + | ::②Double Digesiton:BBa_R0010[http://partsregistry.org/Part:BBa_R0010](2007) |
+ | ::③Single Digestion:BBa_R0010[http://partsregistry.org/Part:BBa_R0010](2007) | ||
+ | ::④BBa_R0010[http://partsregistry.org/Part:BBa_R0010](2007) | ||
+ | <table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000"> | ||
+ | <tr> | ||
+ | <td width="257">Sample No</td> | ||
+ | <td>①</td><td>②</td><td>③</td><td>④</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Sample DNA</td> | ||
+ | <td>5</td><td>5</td><td>1</td><td>1</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>EcoRⅠ</td> | ||
+ | <td>0.5</td><td>o.5</td><td>-</td><td>-</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>SpeⅠ</td> | ||
+ | <td>0.5</td><td>0.5</td><td>-</td><td>-</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Buffer 1</td> | ||
+ | <td>1</td><td>1</td><td>-</td><td>-</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>Buffer 3</td> | ||
+ | <td>-</td><td>-</td><td>1</td><td>-</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BSA</td> | ||
+ | <td>1</td><td>1</td><td>-</td><td>-</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>dH2O</td> | ||
+ | <td>2</td><td>2</td><td>7.5</td><td>-</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>TOTAL</td> | ||
+ | <td>10</td><td>10</td><td>10</td><td>1</td> | ||
+ | </tr> | ||
+ | </table> | ||
:--->[[Team:Chiba/protocol/gelcheck|Gel Check]] | :--->[[Team:Chiba/protocol/gelcheck|Gel Check]] |
Revision as of 15:03, 3 September 2008
β班の実験ノート
Contents |
Week 1
- 17 August, 2008
- 18 August, 2008
- --->(20/8)Mini prep
- 19 August, 2008
- --->Gel Check
- 20 August, 2008
- (18/8)--->Mini prep
- --->Gel Check
Sample No | ① | ② | ③ | ④ |
Sample DNA | 3 | 3 | 3 | 3 |
Loading Dye | 2 | 2 | 2 | 2 |
dH2O | 7 | 7 | 7 | 7 |
TOTAL | 12 | 12 | 12 | 12 |
From the left; |
- 21 August, 2008
- (20/8)--->PCR
- --->Gel Check
From the left; |
- 22 August, 2008
- Transformation
- (Locate & 2 Punch ~iyama ver.~)
- 23 August, 2008
- --->Mini prep
- --->Digestion
- --->Gel Check</table>
From the left; |
|
Week 2
- 24 August, 2008
- (22/8)--->Mini prep
- --->Digestion
- --->Gel Check
Sample No | ① | ② | ③ | ④ | ⑤ | ⑥ | ⑦ | ⑧ |
Sample DNA | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
Loading Dye | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
dH2O | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 |
TOTAL | 6 | 6 | 6 | 6 | 6 | 6 | 6 | 6 |
From the left; (↓single digestuion) |
Sample No | ⑤ | ⑥ | ⑦ | ⑧ |
Sample DNA | 9 | 9 | 9 | 9 |
Loading Dye | 2 | 2 | 2 | 2 |
dH2O | 1 | 1 | 1 | 1 |
TOTAL | 12 | 12 | 12 | 12 |
From the left; (single digestuion) |
- 25 August, 2008
- (24/8)--->Digestion
Sample No | ① | ② | ③ | ④ |
Sample DNA | 5 | 5 | 1 | 1 |
EcoRⅠ | 0.5 | o.5 | - | - |
SpeⅠ | 0.5 | 0.5 | - | - |
Buffer 1 | 1 | 1 | - | - |
Buffer 3 | - | - | 1 | - |
BSA | 1 | 1 | - | - |
dH2O | 2 | 2 | 7.5 | - |
TOTAL | 10 | 10 | 10 | 1 |
- --->Gel Check
Sample No | ① | ② | ③ | ④ |
Sample DNA | 9 | 9 | 9 | 9 |
Loading Dye | 2 | 2 | 2 | 2 |
dH2O | 1 | 1 | 1 | 1 |
TOTAL | 12 | 12 | 12 | 12 |
From left; |
From left; |
From left; |
From left; |
From left; |
- 26 August, 2008
- --->Gel Check
Sample No | ⑤ | ⑥ | ⑦ | ⑧ |
Sample DNA | 9 | 9 | 9 | 9 |
Loading Dye | 2 | 2 | 2 | 2 |
dH2O | 1 | 1 | 1 | 1 |
TOTAL | 12 | 12 | 12 | 12 |
From left; |
- 27 August, 2008
- PCR
- Colony PCR of 5 colonies from ligation plates (27/8) and one from control plate(BBa_F2620[148](2007)).
From left; |
From left; |
- 28 August, 2008]
From left; |
- 29 August, 2008
- 30 August, 2008
Week 3
- 31 August, 2008
- 1 September, 2008
- 2 September, 2008
- 3 September, 2008
- 4 September, 2008
- 5 September, 2008
- 6 September, 2008
Week 4
- 7 September, 2008
- 8 September, 2008
- 9 September, 2008
- 10 September, 2008
ホーム | メンバー紹介 | プロジェクト紹介 | Parts Submitted to the Registry | モデリング | ノート |
---|