Team:Chiba/jk/β

From 2008.igem.org

(Difference between revisions)
(→Week 1)
(→Week 1)
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::*[http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2007)
::*[http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2007)
::*[http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2006)
::*[http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2006)
 +
*19 August, 2008  
*19 August, 2008  
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::*[http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2006)
::*[http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2006)
::*[http://partsregistry.org/Part:BBa_F2620| BBa_F2620](2007)
::*[http://partsregistry.org/Part:BBa_F2620| BBa_F2620](2007)
 +
*20 August, 2008
*20 August, 2008
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::*[http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007)
::*[http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007)
::*[http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006)
::*[http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006)
 +
*21 August, 2008
*21 August, 2008
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::*[http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007) -> None
::*[http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007) -> None
::*[http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006) -> None
::*[http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006) -> None
-
 
:[[Team:Chiba/protocol/transformation|Transforation]]
:[[Team:Chiba/protocol/transformation|Transforation]]
-
(Locate & 2 Punch)
+
:(Locate & 2 Punch ~iyama ver.~)
::*[http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2008) -> No colonies on plates  
::*[http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2008) -> No colonies on plates  
::*[http://partsregistry.org/Part:BBa_C0077| BBa_C0077](2008) -> No colonies on plates  
::*[http://partsregistry.org/Part:BBa_C0077| BBa_C0077](2008) -> No colonies on plates  
::*[http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2008) -> No colonies on plates  
::*[http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2008) -> No colonies on plates  
-
 
*22 August, 2008
*22 August, 2008
 +
*23 August, 2008
*23 August, 2008

Revision as of 13:22, 30 August 2008

β班の実験ノート


Contents

Week 1

  • 17 August, 2008
Transforation
  • [http://partsregistry.org/Part:BBa_R0040| BBa_R0040](2008) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2008) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2008) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0077| BBa_C0077](2008) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2008) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0079| BBa_C0079](2008) -> No colonies on plates


  • 18 August, 2008
Transforation
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007)
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006)
  • [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007)
  • [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2006) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007)
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006)
  • [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2007)
  • [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2006)
  • [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2007)
  • [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2006)


  • 19 August, 2008
PCR
  • [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2007)
  • [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2006)
  • [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2007)
  • [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2006)
  • [http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2007)
  • [http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2006)
  • [http://partsregistry.org/Part:BBa_F2620| BBa_F2620](2007)･･･control
Gel Check
  • [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2007)
  • [http://partsregistry.org/Part:BBa_I9026| BBa_I9026](2006)
  • [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2007)
  • [http://partsregistry.org/Part:BBa_I9030| BBa_I9030](2006)
  • [http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2007)
  • [http://partsregistry.org/Part:BBa_S3154| BBa_S3154](2006)
  • [http://partsregistry.org/Part:BBa_F2620| BBa_F2620](2007)


  • 20 August, 2008
Mini prep
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007)
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006)
  • [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007)
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007)
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006)
Digestion
  • [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2007)
  • [http://partsregistry.org/Part:BBa_C0170| BBa_C0170](2006)
  • [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2007)
  • [http://partsregistry.org/Part:BBa_C0178| BBa_C0178](2006)
Gel Check
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007)
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006)
  • [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007)
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007)
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006)


  • 21 August, 2008
PCR
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007)
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006)
  • [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007)
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007)
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006)
Gel Check
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2007)
  • [http://partsregistry.org/Part:BBa_C0070| BBa_C0070](2006)
  • [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2007) -> None
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2007) -> None
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2006) -> None
Transforation
(Locate & 2 Punch ~iyama ver.~)
  • [http://partsregistry.org/Part:BBa_C0076| BBa_C0076](2008) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0077| BBa_C0077](2008) -> No colonies on plates
  • [http://partsregistry.org/Part:BBa_C0078| BBa_C0078](2008) -> No colonies on plates


  • 22 August, 2008


  • 23 August, 2008

Week 2

  • 27 August, 2008
Colony PCR of 5 colonies from ligation plates (27/8) and one from control plate([http://partsregistry.org/Part:BBa_F2620| BBa_F2620](2007)).

Week 3

reference

  • L. Steindler.V.Venturi.:Detection of quorum sensing N-acyl homoserine lactone.FEMS Microbiol Lett 266 (2007) 1-9
クオラムセンシングのクロストークについてのミニレビュー。クオラムセンシングを利用したAHLバイオセンサーについて、Table 1.にまとまっている。
  • M.K Winson et al./FEMS Microbiology Letters 163 (1998) 185-192
Figure 2.hは、LuxR、RhlR、LasRそれぞれの、C4-C14のN-acyl-L-homoserine lactone,C4-C14のN-(3-oxoacyl)-L-homoserine lactoneに対する感受性を比較したグラフ。


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