Team:Chiba/jk/β

From 2008.igem.org

(Difference between revisions)

Revision as of 15:23, 3 September 2008

β班の実験ノート

Week 1

17 August, 2008

Transformation

BBa_R0040(2008) [http://partsregistry.org/Part:BBa_R0040] -> No colonies on plates
BBa_C0070(2008) [http://partsregistry.org/Part:BBa_C0070] -> No colonies on plates
BBa_C0076(2008) [http://partsregistry.org/Part:BBa_C0076] -> No colonies on plates
BBa_C0077(2008) [http://partsregistry.org/Part:BBa_C0077] -> No colonies on plates
BBa_C0078(2008) [http://partsregistry.org/Part:BBa_C0078] -> No colonies on plates
BBa_C0079(2008) [http://partsregistry.org/Part:BBa_C0079] -> No colonies on plates

18 August, 2008

Transformation

BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070]
BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070]
BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076]
BBa_C0076(2006) [http://partsregistry.org/Part:BBa_C0076] -> No colonies on plates
BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078]
BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078]
BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170]
BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170]
BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178]
BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178]

--->(20/8)Mini prep

19 August, 2008

PCR

BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
BBa_S3154(2007) [http://partsregistry.org/Part:BBa_S3154]
BBa_S3154(2006) [http://partsregistry.org/Part:BBa_S3154]
BBa_F2620(2007) [http://partsregistry.org/Part:BBa_F2620]･･･control

--->Gel Check

BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026] ->None
BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026] ->None
BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030] ->None
BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030] ->None
BBa_S3154(2007) [http://partsregistry.org/Part:BBa_S3154] ->None
BBa_S3154(2006) [http://partsregistry.org/Part:BBa_S3154] ->None
BBa_F2620(2007) [http://partsregistry.org/Part:BBa_F2620]･･･control ->OK

20 August, 2008

(18/8)--->Mini prep

BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070]
BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070]
BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076]
BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078]
BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078]
BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170]
BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170]
BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178]
BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178]

--->Gel Check

Sample No	①	②	③	④
Sample DNA	3	3	3	3
Loading Dye	2	2	2	2
dH2O	7	7	7	7
TOTAL	12	12	12	12

From the left;
①BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170] -> OK
②BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170] -> OK
③BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178] -> OK
④BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178] -> OK

BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170] -> OK
BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170] -> OK
BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178] -> OK
BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178] -> OK

BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076] -> None
BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078] -> None
BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078] -> None

BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078] -> None
BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078] -> OK
BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076] -> None
BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070] -> OK
BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070] -> OK

--->Digestion

BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070]
BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070]
BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078]
BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078]

21 August, 2008

(20/8)--->PCR

BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070]
BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070]
BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076]
BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078]
BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078]

--->Gel Check

From the left;

BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078] -> None
BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078] -> None
BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076] -> None
BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070] -> OK
BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070] -> OK

Transformation

BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]

22 August, 2008

Transformation

BBa_J13002(2007) [http://partsregistry.org/Part:BBa_J13002]
BBa_J13002(2006) [http://partsregistry.org/Part:BBa_J13002]
BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]
BBa_J04500(2006) [http://partsregistry.org/Part:BBa_J04500]

(Locate & 2 Punch ~iyama ver.~)

BBa_C0076(2008) [http://partsregistry.org/Part:BBa_C0076] -> No colonies on plates
BBa_C0077(2008) [http://partsregistry.org/Part:BBa_C0077] -> No colonies on plates
BBa_C0078(2008) [http://partsregistry.org/Part:BBa_C0078] -> No colonies on plates

23 August, 2008

--->Mini prep

BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]

--->Digestion

BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]

--->Gel Check</table>

From the left;
①BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170] -> OK
②BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170] -> OK
③BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178] -> OK
④BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178] -> OK

BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]

BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]
M
BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]

BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]
BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]Single Digestion
BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]Double Digestion

Week 2

24 August, 2008

(22/8)--->Mini prep

BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2007)
BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2006)
BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007)
BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2006)

--->Digestion

BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2007)
BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2006)
BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007)
BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2006)

--->Gel Check

Sample No	①	②	③	④	⑤	⑥	⑦	⑧
Sample DNA	1	1	1	1	1	1	1	1
Loading Dye	1	1	1	1	1	1	1	1
dH2O	4	4	4	4	4	4	4	4
TOTAL	6	6	6	6	6	6	6	6

From the left;

⑤BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007)

⑥BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2006)

⑦BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2007)

⑧BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2006)

（↓single digestuion）

⑤BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007)

⑥BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2006)

⑦BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2007)

⑧BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2006)

Sample No	⑤	⑥	⑦	⑧
Sample DNA	9	9	9	9
Loading Dye	2	2	2	2
dH2O	1	1	1	1
TOTAL	12	12	12	12

From the left; （single digestuion）

⑤BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007)

⑥BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2006)

⑦BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2007)

⑧BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2006)

25 August, 2008

(24/8)--->Digestion

①Double Digestion:BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007)

②Double Digesiton:BBa_R0010[http://partsregistry.org/Part:BBa_R0010](2007)

③Single Digestion:BBa_R0010[http://partsregistry.org/Part:BBa_R0010](2007)

④BBa_R0010[http://partsregistry.org/Part:BBa_R0010](2007)

Sample No	①	②	③	④
Sample DNA	5	5	1	1
EcoRⅠ	0.5	o.5	-	-
SpeⅠ	0.5	0.5	-	-
Buffer 1	1	1	-	-
Buffer 3	-	-	1	-
BSA	1	1	-	-
dH2O	2	2	7.5	-
TOTAL	10	10	10	1

--->Gel Check

Sample No	①	②	③	④
Sample DNA	10	10	10	10
Loading Dye	2	2	2	1
dH2O	-	-	-	4
TOTAL	12	12	12	6

From right;

①Double Digestion:BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007) -> OK

②Double Digesiton:BBa_R0010[http://partsregistry.org/Part:BBa_R0010](2007) -> ?(low)

③Single Digestion:BBa_R0010[http://partsregistry.org/Part:BBa_R0010](2007) -> ?(low)

④BBa_R0010[http://partsregistry.org/Part:BBa_R0010](2007) -> OK(low)

)--->Digestion

①BBa_C0178[http://partsregistry.org/Part:BBa_C0178]

②BBa_C0170[http://partsregistry.org/Part:BBa_C0170]

③BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007)

]

</tr>

Sample No	①	②	③
Sample DNA	33ng/μl×60μl	33ng/μl×60μl	100ng/μl×20μl
PstⅠ	2	2	2
XbaⅠ	2	2	-
SpeⅠ	-	-	2
Buffer 2	-	-	5
Buffer 3	10	10	-
BSA	10	10	5
dH2O	16	16	16
TOTAL	100	100	52

--->Gel Check

Sample No	①	②	③
Sample DNA	30	30	30
Loading Dye	6	6	6
TOTAL	36	36	36

①BBa_C0178[http://partsregistry.org/Part:BBa_C0178]

Insert:609bp -> OK

②BBa_C0170[http://partsregistry.org/Part:BBa_C0170]

Insert:609bp -> OK

③BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007)

-> OK

From left;

26 August, 2008

--->Gel Check

Sample No	⑤	⑥	⑦	⑧
Sample DNA	9	9	9	9
Loading Dye	2	2	2	2
dH2O	1	1	1	1
TOTAL	12	12	12	12

From left;

27 August, 2008

PCR

Colony PCR of 5 colonies from ligation plates (27/8) and one from control plate(BBa_F2620[http://partsregistry.org/Part:BBa_F2620](2007)).

From left;

From left;

28 August, 2008]

From left;

29 August, 2008
30 August, 2008

Week 3

31 August, 2008

1 September, 2008

2 September, 2008

3 September, 2008

4 September, 2008

5 September, 2008

6 September, 2008

Week 4

7 September, 2008

8 September, 2008

9 September, 2008

10 September, 2008

ホーム	メンバー紹介	プロジェクト紹介	Parts Submitted to the Registry	モデリング	ノート

@@ Line 395: / Line 395: @@
 <tr>
 <td>Sample DNA</td>
-<td>9</td><td>9</td><td>9</td><td>9</td>
+<td>10</td><td>10</td><td>10</td><td>10</td>
 </tr>
 <tr>
 <td>Loading Dye</td>
-<td>2</td><td>2</td><td>2</td><td>2</td>
+<td>2</td><td>2</td><td>2</td><td>1</td>
 </tr>
 <tr>
 <td>dH2O</td>
-<td>1</td><td>1</td><td>1</td><td>1</td>
+<td>-</td><td>-</td><td>-</td><td>4</td>
 </tr>
 <tr>
 <td>TOTAL</td>
-<td>12</td><td>12</td><td>12</td><td>12</td>
+<td>12</td><td>12</td><td>12</td><td>6</td>
 </tr>
 </table>
@@ Line 413: / Line 413: @@
 |[[Image:Chiba-0825-1.JPG]]
 |
-From left;
+From right;
+::①Double Digestion:BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007) -> OK
+::②Double Digesiton:BBa_R0010[http://partsregistry.org/Part:BBa_R0010](2007) -> ?(low)
+::③Single Digestion:BBa_R0010[http://partsregistry.org/Part:BBa_R0010](2007) -> ?(low)
+::④BBa_R0010[http://partsregistry.org/Part:BBa_R0010](2007) -> OK(low)
 |}
+)--->[[Team:Chiba/protocol/digestion|Digestion]]
+::①BBa_C0178[http://partsregistry.org/Part:BBa_C0178]
+::②BBa_C0170[http://partsregistry.org/Part:BBa_C0170]
+::③BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007)
+<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+<tr>
+<td width="257">Sample No</td>
+<td>①</td><td>②</td><td>③</td>
+</tr>
+<tr>
+<td>Sample DNA</td>
+<td>33ng/μl×60μl</td><td>33ng/μl×60μl</td><td>100ng/μl×20μl</td>
+</tr>
+<tr>
+<td>PstⅠ</td>
+<td>2</td><td>2</td><td>2</td>
+</tr>
+<tr>
+<td>XbaⅠ</td>
+<td>2</td><td>2</td><td>-</td>
+</tr>]
+<td>SpeⅠ</td>
+<td>-</td><td>-</td><td>2</td>
+</tr>
+<tr>
+<td>Buffer 2</td>
+<td>-</td><td>-</td><td>5</td>
+</tr>
+<tr>
+<td>Buffer 3</td>
+<td>10</td><td>10</td><td>-</td>
+</tr>
+<tr>
+<td>BSA</td>
+<td>10</td><td>10</td><td>5</td>
+</tr>
+<tr>
+<td>dH2O</td>
+<td>16</td><td>16</td><td>16</td>
+</tr>
+<tr>
+<td>TOTAL</td>
+<td>100</td><td>100</td><td>52</td>
+</tr>
+</table>
+:--->[[Team:Chiba/protocol/gelcheck|Gel Check]]
+<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+<tr>
+<td width="257">Sample No</td>
+<td>①</td><td>②</td><td>③</td>
+</tr>
+<tr>
+<td>Sample DNA</td>
+<td>30</td><td>30</td><td>30</td>
+</tr>
+<tr>
+<td>Loading Dye</td>
+<td>6</td><td>6</td><td>6</td>
+</tr>
+<tr>
+<td>TOTAL</td>
+<td>36</td><td>36</td><td>36</td>
+</tr>
+</table>
 {|align="justify"
 |[[Image:Chiba-0825-2.JPG]]
 |
-From left;
+::①BBa_C0178[http://partsregistry.org/Part:BBa_C0178]
+:::Insert:609bp -> OK
 |}
 {|align="justify"
 |[[Image:Chiba-0825-3.JPG]]
 |
-From left;
+::②BBa_C0170[http://partsregistry.org/Part:BBa_C0170]
+:::Insert:609bp -> OK
 |}
 {|align="justify"
 |[[Image:Chiba-0825-4.JPG]]
 |
-From left;
+::③BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007)
+::: -> OK
 |}
 {|align="justify"

Team:Chiba/jk/β

From 2008.igem.org

Revision as of 15:23, 3 September 2008

Contents

Week 1

Week 2

Week 3

Week 4