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Team:Chiba/jk/β
From 2008.igem.org
β班の実験ノート
Contents |
Week 1
- 17 August, 2008
- Transforation
- BBa_R0040(2008) -> No colonies on plates
- BBa_C0070(2008) -> No colonies on plates
- BBa_C0076(2008) -> No colonies on plates
- BBa_C0077(2008) -> No colonies on plates
- BBa_C0078(2008) -> No colonies on plates
- BBa_C0079(2008) -> No colonies on plates
- 18 August, 2008
- Transformation
- BBa_C0070(2007)
- BBa_C0070(2006)
- BBa_C0076(2007)
- BBa_C0076(2006) -> No colonies on plates
- BBa_C0078(2007)
- BBa_C0078(2006)
- BBa_C0170(2007)
- BBa_C0170(2006)
- BBa_C0178(2007)
- BBa_C0178(2006)
- 19 August, 2008
- PCR
- ①BBa_I9026(2007)
- ②BBa_I9026(2006)
- ③BBa_I9030(2007)
- ④BBa_I9030(2006)
- ⑤BBa_S03154(2007)
- ⑥BBa_S03154(2006)
- ⑦BBa_F2620(2007)
- Gel Check
- ①～②
- 20 August, 2008
- Sigma prep
- BBa_C0070(2007)
- BBa_C0070(2006)
- BBa_C0076(2006)
- BBa_C0078(2007)
- BBa_C0078(2006)
- Digestion Test
- BBa_C0170(2007)
- BBa_C0170(2006)
- BBa_C0178(2007)
- BBa_C0178(2006)
- 21 August, 2008
- PCR
- BBa_C0070(2007)
- BBa_C0070(2006)
- BBa_C0076(2006)
- BBa_C0078(2007)
- BBa_C0078(2006)
Week 2
- 27 August, 2008
- Colony PCR of 5 colonies from ligation plates (27/8) and one from control plate(F2620).
Week 3
reference
- L. Steindler.V.Venturi.:Detection of quorum sensing N-acyl homoserine lactone.FEMS Microbiol Lett 266 (2007) 1-9
- クオラムセンシングのクロストークについてのミニレビュー。クオラムセンシングを利用したAHLバイオセンサーについて、Table 1.にまとまっている。
- M.K Winson et al./FEMS Microbiology Letters 163 (1998) 185-192
- Figure 2.hは、LuxR、RhlR、LasRそれぞれの、C4-C14のN-acyl-L-homoserine lactone,C4-C14のN-(3-oxoacyl)-L-homoserine lactoneに対する感受性を比較したグラフ。
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