Team:Chiba/jk/β

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β班の実験ノート

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Contents

Week 1

  • 17 August, 2008
Transformation
  • BBa_R0040(2008) [1] -> No colonies on plates
  • BBa_C0070(2008) [2] -> No colonies on plates
  • BBa_C0076(2008) [3] -> No colonies on plates
  • BBa_C0077(2008) [4] -> No colonies on plates
  • BBa_C0078(2008) [5] -> No colonies on plates
  • BBa_C0079(2008) [6] -> No colonies on plates


  • 18 August, 2008
Transformation
  • BBa_C0070(2007) [7]
  • BBa_C0070(2006) [8]
  • BBa_C0076(2007) [9]
  • BBa_C0076(2006) [10] -> No colonies on plates
  • BBa_C0078(2007) [11]
  • BBa_C0078(2006) [12]
  • BBa_C0170(2007) [13]
  • BBa_C0170(2006) [14]
  • BBa_C0178(2007) [15]
  • BBa_C0178(2006) [16]


--->(20/8)Mini prep


  • 19 August, 2008
PCR
  • BBa_I9026(2007) [17]
  • BBa_I9026(2006) [18]
  • BBa_I9030(2007) [19]
  • BBa_I9030(2006) [20]
  • BBa_S3154(2007) [21]
  • BBa_S3154(2006) [22]
  • BBa_F2620(2007) [23]･･･control


--->Gel Check
  • BBa_I9026(2007) [24]
  • BBa_I9026(2006) [25]
  • BBa_I9030(2007) [26]
  • BBa_I9030(2006) [27]
  • BBa_S3154(2007) [28]
  • BBa_S3154(2006) [29]
  • BBa_F2620(2007) [30]･･･control


  • 20 August, 2008
(18/8)--->Mini prep
  • BBa_C0070(2007) [31]
  • BBa_C0070(2006) [32]
  • BBa_C0076(2007) [33]
  • BBa_C0078(2007) [34]
  • BBa_C0078(2006) [35]
  • BBa_C0170(2007) [36]
  • BBa_C0170(2006) [37]
  • BBa_C0178(2007) [38]
  • BBa_C0178(2006) [39]


--->Gel Check
  • BBa_C0070(2007) [40] -> OK
  • BBa_C0070(2006) [41] -> OK
  • BBa_C0076(2007) [42] -> None
  • BBa_C0078(2007) [43] -> None
  • BBa_C0078(2006) [44] -> OK
  • BBa_C0170(2007) [45] -> OK
  • BBa_C0170(2006) [46] -> OK
  • BBa_C0178(2007) [47] -> OK
  • BBa_C0178(2006) [48] -> OK

Chiba-0818.JPG


--->Digestion
  • BBa_C0070(2007) [49]
  • BBa_C0070(2006) [50]
  • BBa_C0078(2007) [51]
  • BBa_C0078(2006) [52]


  • 21 August, 2008
(20/8)--->PCR
  • BBa_C0070(2007) [53]
  • BBa_C0070(2006) [54]
  • BBa_C0076(2007) [55]
  • BBa_C0078(2007) [56]
  • BBa_C0078(2006) [57]


--->Gel Check
  • BBa_C0070(2007) [58] -> OK
  • BBa_C0070(2006) [59] -> OK
  • BBa_C0076(2007) [60] -> None
  • BBa_C0078(2007) [61] -> None
  • BBa_C0078(2006) [62] -> None


Transformation
  • BBa_S03154(2007) [63]
  • BBa_S03154(2006) [64]
  • BBa_I9026(2007) [65]
  • BBa_I9026(2006) [66]
  • BBa_I9030(2007) [67]
  • BBa_I9030(2006) [68]


  • 22 August, 2008
Transformation
  • BBa_J13002(2007) [69]
  • BBa_J13002(2006) [70]
  • BBa_J04500(2007) [71]
  • BBa_J04500(2006) [72]
(Locate & 2 Punch ~iyama ver.~)
  • BBa_C0076(2008) [73] -> No colonies on plates
  • BBa_C0077(2008) [74] -> No colonies on plates
  • BBa_C0078(2008) [75] -> No colonies on plates



  • 23 August, 2008
--->Mini prep
  • BBa_S03154(2007) [76]
  • BBa_S03154(2006) [77]
  • BBa_I9026(2007) [78]
  • BBa_I9026(2006) [79]
  • BBa_I9030(2007) [80]
  • BBa_I9030(2006) [81]


--->Digestion
  • BBa_S03154(2007) [82]
  • BBa_S03154(2006) [83]
  • BBa_I9026(2007) [84]
  • BBa_I9026(2006) [85]
  • BBa_I9030(2007) [86]
  • BBa_I9030(2006) [87]
  • BBa_J04500(2007) [88]


--->Gel Check
  • BBa_S03154(2007) [89]
  • BBa_S03154(2006) [90]
  • BBa_I9026(2007) [91]
  • BBa_I9026(2006) [92]
  • BBa_I9030(2007) [93]
  • BBa_I9030(2006) [94]

Week 2

  • 24 August, 2008
(22/8)--->Mini prep
  • BBa_J13002[95](2007)
  • BBa_J13002[96](2006)
  • BBa_J04500[97](2007)
  • BBa_J04500[98](2006)
--->Digestion
--->Gel Check
  • 25 August, 2008
(24/8)--->Digestion


--->Gel Check


  • 26 August, 2008


  • 27 August, 2008
PCR
Colony PCR of 5 colonies from ligation plates (27/8) and one from control plate(BBa_F2620[111](2007)).


  • 30 August, 2008

Week 3

  • 31 August, 2008


  • 1 September, 2008

Week 4

ホーム メンバー紹介 プロジェクト紹介 Parts Submitted to the Registry モデリング ノート
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