Team:Chiba/jk/β/weak 3

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(Week 3)
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>[[Team:Chiba/jk/β|送受信班]]
 
-
==Week 3==
 
-
===[[Team:Chiba/jk/β/weak 2|>last week]]===
 
-
===31 August, 2008===
 
-
:'''[[Team:Chiba/protocol/transformation|Transformation]]'''
 
-
:Competent Cells : XL10G
 
-
::*Plac+RBS+LasI
 
-
::*Plac+RBS+RhlI
 
-
 
-
'''[[Team:Chiba/protocol/digestion|Digestion]]'''
 
-
::#BBa_I9026[http://partsregistry.org/Part:BBa_I9026](2007)
 
-
::#BBa_I9030[http://partsregistry.org/Part:BBa_I9030](2006)
 
-
::#BBa_S03154[http://partsregistry.org/Part:BBa_S03154](2007)
 
-
::#BBa_R0010[http://partsregistry.org/Part:BBa_R0010](2007)
 
-
 
-
<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 
-
<tr>
 
-
<td width="257">Sample No</td>
 
-
<td>1~3</td><td>4</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Sample DNA</td><td>12</td><td>10</td>
 
-
</tr>
 
-
<tr>
 
-
<td>PstⅠ</td>
 
-
<td>0.2</td><td>0.2</td>
 
-
</tr>
 
-
<tr>
 
-
<td>XbaⅠ</td><td>0.2</td><td>-</td>
 
-
</tr>
 
-
<tr>
 
-
<td>SpeⅠ</td><td>-</td><td>0.4</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Buffer 2</td>
 
-
<td>-</td><td>1.5</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Buffer 3</td>
 
-
<td>2</td><td>-</td>
 
-
</tr>
 
-
<tr>
 
-
<td>BSA</td>
 
-
<td>2</td><td>1.5</td>
 
-
</tr>
 
-
<tr>
 
-
<td>dH2O</td>
 
-
<td>3.6</td><td>1.4</td></tr>
 
-
<tr>
 
-
<td>TOTAL</td>
 
-
<td>20</td><td>15</td>
 
-
</tr>
 
-
</table>
 
-
 
-
 
-
:--->(1/9)'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
 
-
 
-
 
-
:(30/8)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''
 
-
 
-
 
-
:--->'''[[Team:Chiba/protocol/digestion|Digestion test]]'''
 
-
:*Plac+RBS+RhlI Sample No.2~5
 
-
:*BBa_T9002[http://partsregistry.org/Part:BBa_T9002]①、②
 
-
 
-
<table width="600" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 
-
<tr>
 
-
<td width="257">Sample</td>
 
-
<td>Single Digesiton 2~5</td><td>Double Digestion 2~5</td><td>Single Digestion T9002①</td><td>Single Digesiton T9002②</td><td><Double Digestion T9002①②</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Sample DNA</td><td>1</td><td>3</td><td>3</td><td>1</td><td>3</td>
 
-
</tr>
 
-
<tr>
 
-
<td>XbaⅠ</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
 
-
</tr>
 
-
<tr>
 
-
<td>SpeⅠ</td><td>-</td><td>0.1</td><td>-</td><td>-</td><td>0.1</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Buffer 2</td>
 
-
<td>0.9</td><td>0.8</td><td>0.9</td><td>0.9</td><td>0.8</td>
 
-
</tr>
 
-
<tr>
 
-
<td>BSA</td>
 
-
<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
 
-
</tr>
 
-
<tr>
 
-
<td>dH2O</td>
 
-
<td>7</td><td>5</td><td>5</td><td>7</td><td>5</td>
 
-
</tr>
 
-
<tr>
 
-
<td>TOTAL</td>
 
-
<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
 
-
</tr>
 
-
</table>
 
-
 
-
 
-
--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
 
-
 
-
{|align="justify"
 
-
|[[Image:Chiba-0831-p87.JPG]]
 
-
|
 
-
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 
-
<tr>
 
-
<td width="257">Sample No</td>
 
-
<td>Plac+RBS+RhlI Sample No.2~5, BBa_T9002[http://partsregistry.org/Part:BBa_T9002]①②
 
-
</td><td>Single & Double Digestion</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Sample DNA</td>
 
-
<td>1</td><td>10</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Loading Dye</td>
 
-
<td>1</td><td>2</td></tr>
 
-
<tr>
 
-
<td>dH2O</td>
 
-
<td>4</td><td>-</td>
 
-
</tr>
 
-
<tr>
 
-
<td>TOTAL</td>
 
-
<td>6</td><td>12</td>
 
-
</tr>
 
-
</table>
 
-
:From Left
 
-
::*Single Digestion Plac+RBS+RhlI 2~5
 
-
::*Single Digestion BBa_T9002[http://partsregistry.org/Part:BBa_T9002]①、②
 
-
::*Double Digestion Plac+RBS+RhlI 2~5
 
-
::*Double Digestion BBa_T9002[http://partsregistry.org/Part:BBa_T9002]①、②
 
-
::*BBa_T9002[http://partsregistry.org/Part:BBa_T9002]①、②
 
-
|}
 
-
 
-
===1 September, 2008===
 
-
:(31/8)--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
 
-
{|align="justify"
 
-
|[[Image:Chiba-0901.JPG]]
 
-
|
 
-
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 
-
<tr>
 
-
<td width="257">Sample No</td>
 
-
<td>1~3</td><td>4</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Sample DNA</td>
 
-
<td>20</td><td>15</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Loading Dye</td>
 
-
<td>4</td><td>3</td></tr>
 
-
<tr>
 
-
<td>TOTAL</td>
 
-
<td>24</td><td>18</td>
 
-
</tr>
 
-
</table>
 
-
:From left;
 
-
::insert-1(I9026)
 
-
::insert-2(I9030)
 
-
|}
 
-
{|align="justify"
 
-
|[[Image:Chiba-0901-2.JPG]]
 
-
|
 
-
::insert-3(S03154)
 
-
|}
 
-
{|align="justify"
 
-
|[[Image:Chiba-0901-3.JPG]]
 
-
|
 
-
::vector-4(R0010)
 
-
|}
 
-
 
-
:--->zymo
 
-
::vector-4(R0010)
 
-
::-> 15μL
 
-
 
-
:--->SAP
 
-
::vectr-4(R0010)
 
-
 
-
:--->Zymo
 
-
::1 -> 7μL
 
-
::2 -> 7
 
-
::3 -> 7
 
-
::4 -> 20
 
-
 
-
 
-
 
-
'''[[Team:Chiba/protocol/transformation|Transformation]]'''
 
-
:Competent cells : BW ΔFliC  40μL
 
-
::*Plac+RBS+LasI : Sample No.L1~L4
 
-
::*Plac+RBS+RhlI : Sample No.R1~R4
 
-
 
-
:--->(2/9)OD
 
-
 
-
===2 September, 2008===
 
-
:(31/8)--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
 
-
 
-
{|align="justify"
 
-
|[[Image:Chiba-0902.JPG]]
 
-
|
 
-
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 
-
<tr>
 
-
<td width="257">Sample No</td>
 
-
<td>①</td><td>②</td><td>③</td><td>④</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Sample DNA</td>
 
-
<td>3</td><td>3</td><td>3</td><td>3</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Loading Dye</td>
 
-
<td>2</td><td>2</td><td>2</td><td>2</td>
 
-
</tr>
 
-
<tr>
 
-
<td>dH2O</td>
 
-
<td>7</td><td>7</td><td>7</td><td>7</td>
 
-
</tr>
 
-
<tr>
 
-
<td>TOTAL</td>
 
-
<td>12</td><td>12</td><td>12</td><td>12</td>
 
-
</tr>
 
-
</table>
 
-
:From left;
 
-
::I9026
 
-
::I9030
 
-
::S03154
 
-
|}
 
-
 
-
 
-
 
-
:(1/9)--->'''[[Team:Chiba/protocol/PCR|Colony-PCR]]'''
 
-
::Colony PCR of 8 colonies from ligation plates (1/9-(1),(2)) and one from control plate(BBa_F2620[http://partsregistry.org/Part:BBa_F2620](2007)).
 
-
 
-
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 
-
<tr>
 
-
<td width="257">DNA Template</td>
 
-
<td>1</td>
 
-
</tr>
 
-
<tr>
 
-
<td>dNTP mix</td>
 
-
<td>5</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Foward Primer</td>
 
-
<td>0.3</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Reverse Primer</td>
 
-
<td>0.3</td>
 
-
</tr>
 
-
<tr>
 
-
<td>DNA polymerase TAQ</td>
 
-
<td>0.5</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Thermopol Buffer</td>
 
-
<td>3</td>
 
-
</tr>
 
-
<tr>
 
-
<td>dH2O</td>
 
-
<td>20.5</td>
 
-
</tr>
 
-
<tr>
 
-
<td>TOTAL</td>
 
-
<td>30μL</td>
 
-
</tr>
 
-
</table>
 
-
 
-
 
-
:95℃,5min -> ( 95℃,1min -> 52℃,1min -> 72℃,1min )・・・25cycles -> 72℃,10min -> 6℃
 
-
 
-
 
-
--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
 
-
 
-
{|align="justify"
 
-
|[[Image:Chiba-0902-2.JPG]]
 
-
|
 
-
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 
-
<tr>
 
-
<td width="257">Sample DNA</td>
 
-
<td>1</td></tr>
 
-
<tr>
 
-
<td>Loading Dye</td>
 
-
<td>1</td>
 
-
</tr>
 
-
<tr>
 
-
<td>dH2O</td>
 
-
<td>4</td>
 
-
</tr>
 
-
<tr>
 
-
<td>TOTAL</td>
 
-
<td>6</td>
 
-
</tr>
 
-
</table>
 
-
:From left;
 
-
:*Plac+RBS+RhlI+LVA
 
-
::R1 -> OK
 
-
::R2 -> Bad
 
-
::R3~R7 -> OK
 
-
::R8 -> Bad
 
-
|}
 
-
 
-
{|align="justify"
 
-
|[[Image:Chiba-0902-3.JPG]]
 
-
|
 
-
:From left;
 
-
:*Plac+RBS+CinI+LVA
 
-
::C1,C2 -> OK
 
-
::C3 -> Bad
 
-
::C4~C6 -> OK
 
-
|}
 
-
 
-
{|align="justify"
 
-
|[[Image:Chiba-0902-4.JPG]]
 
-
|
 
-
:From left;
 
-
:*Plac+RBS+CinI+LVA
 
-
::C7,C8 -> OK
 
-
:*BBa_F2620[http://partsregistry.org/Part:BBa_F2620](2007):Positive control -> OK
 
-
|}
 
-
 
-
 
-
:--->(3/9)'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''
 
-
 
-
 
-
 
-
 
-
:(1/9)--->OD測
 
-
 
-
 
-
 
-
'''[[Team:Chiba/protocol/transformation|Transformation]]'''
 
-
:Competent cells : XL10G  30μL
 
-
::*C0161(2007) [http://partsregistry.org/Part:BBa_C0161]
 
-
::*C0161(2006) [http://partsregistry.org/Part:BBa_C0161]
 
-
::*C0261(2007) [http://partsregistry.org/Part:BBa_C0261]
 
-
::*C0261(2006) [http://partsregistry.org/Part:BBa_C0261]
 
-
 
-
:--->(4/9)'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''
 
-
 
-
===3 September, 2008===
 
-
:(2/9)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''
 
-
 
-
:--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
 
-
{|align="justify"
 
-
|[[Image:Chiba-0903.JPG]]
 
-
|
 
-
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 
-
<tr>
 
-
<td width="257">Sample DNA</td>
 
-
<td>1</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Loading Dye</td>
 
-
<td>1</td>
 
-
</tr>
 
-
<tr>
 
-
<td>dH2O</td>
 
-
<td>4</td>
 
-
</tr>
 
-
<tr>
 
-
<td>TOTAL</td>
 
-
<td>6</td>
 
-
</tr>
 
-
</table>
 
-
:From left;
 
-
::*S03154 -> OK
 
-
::*R1,R3~R7  (Plac+RBS+RhlI+LVA) -> OK
 
-
|}
 
-
 
-
{|align="justify"
 
-
|[[Image:Chiba-0903-2.JPG]]
 
-
|
 
-
:From left;
 
-
::*C1,C2,C4~C8  (Plac+RBS+CinI+LVA) -> OK
 
-
|}
 
-
 
-
:--->'''[[Team:Chiba/protocol/digestion|Digestion test]]'''
 
-
::*R1,R3~R7  (Plac+RBS+RhlI+LVA)
 
-
::*C1,C2,C4~C8  (Plac+RBS+CinI+LVA)
 
-
 
-
<table width="500" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 
-
<tr>
 
-
<td width="257">Digestion</td>
 
-
<td>Single</td><td>Double</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Sample DNA</td>
 
-
<td>1</td><td>3</td>
 
-
</tr>
 
-
<tr>
 
-
<td>XbaⅠ</td>
 
-
<td>0.1</td><td>0.1</td>
 
-
</tr>
 
-
<tr>
 
-
<td>PstⅠ</td>
 
-
<td>0.1</td><td>0.1</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Buffer 2</td>
 
-
<td>0.9</td><td>-</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Buffer 3</td>
 
-
<td>-</td><td>-0.8</td>
 
-
</tr>
 
-
<tr>
 
-
<td>BSA</td>
 
-
<td>1</td><td>1</td>
 
-
</tr>
 
-
<tr>
 
-
<td>dH2O</td>
 
-
<td>7</td><td>5</td></tr>
 
-
<tr>
 
-
<td>TOTAL</td>
 
-
<td>10</td><td>10</td>
 
-
</tr>
 
-
</table>
 
-
 
-
 
-
:--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
 
-
{|align="justify"
 
-
|[[Image:Chiba-0903-3.JPG]]
 
-
|
 
-
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 
-
<tr>
 
-
<td width="257">Sample DNA</td>
 
-
<td>10</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Loading Dye</td>
 
-
<td>2</td></tr>
 
-
<tr><tr>
 
-
<td>TOTAL</td>
 
-
<td>12</td>
 
-
</tr>
 
-
</table>
 
-
:From left;
 
-
:*Single Digestion : R1,R3~R7 -> OK
 
-
:*Single Digestion : C1,C2,C4~C8 -> OK
 
-
|}
 
-
 
-
{|align="justify"
 
-
|[[Image:Chiba-0903-4.JPG]]
 
-
|
 
-
:From left;
 
-
:*Double Digestion : R1,R3~R7 -> OK
 
-
:*Double Digestion : C1,C2,C4~C8 -> OK
 
-
|}
 
-
 
-
 
-
(2/9)--->'''Phenotype-test : mix'''
 
-
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 
-
<tr>
 
-
<td width="257">Sample No.</td>
 
-
<td>1</td><td>2</td><td>3</td><td>4</td><td>5</td><td>6</td><td>7</td><td>8</td><td>9</td><td>10</td><td>11</td>
 
-
</tr>
 
-
<tr>
 
-
<td>L1</td>
 
-
<td>2</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td>
 
-
</tr>
 
-
<tr>
 
-
<tr><td>L2</td><td>-</td><td>2</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td></tr>
 
-
<tr><td>L3</td><td>-</td><td>-</td><td>2</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td></tr>
 
-
<tr><td>L4</td><td>-</td><td>-</td><td>-</td><td>2</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td></tr>
 
-
<tr><td>R1</td><td>-</td><td>-</td><td>-</td><td>-</td><td>1</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td></tr>
 
-
<tr><td>R2</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>1</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td></tr>
 
-
<tr><td>R3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>1</td><td>-</td><td>-</td><td>-</td><td>-</td></tr>
 
-
<tr><td>R4</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>1</td><td>-</td><td>-</td><td>-</td></tr>
 
-
 
-
<tr><td>BBa_S03154[http://partsregistry.org/Part:BBa_S03154]</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>2</td><td>-</td><td>-</td></tr>
 
-
<tr><td>BBa_T9002[http://partsregistry.org/Part:BBa_T9002]</td><td>2</td><td>2</td><td>2</td><td>2</td><td>1</td><td>1</td><td>1</td><td>1</td><td>2</td><td>1</td><td>1</td></tr>
 
-
<tr><td>AHL</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td></tr>
 
-
</table>
 
-
:::|
 
-
:::| 8h
 
-
:::V
 
-
::Spindown
 
-
:::|
 
-
:::|
 
-
:::V
 
-
::the intensity GFP
 
-
 
-
===4 September, 2008===
 
-
 
-
:--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
 
-
{|align="justify"
 
-
|[[Image:Chiba-0904.JPG]]
 
-
|
 
-
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 
-
<tr>
 
-
<td width="257">Sample No</td>
 
-
<td>①</td><td>②</td><td>③</td><td>④</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Sample DNA</td>
 
-
<td>3</td><td>3</td><td>3</td><td>3</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Loading Dye</td>
 
-
<td>2</td><td>2</td><td>2</td><td>2</td>
 
-
</tr>
 
-
<tr>
 
-
<td>dH2O</td>
 
-
<td>7</td><td>7</td><td>7</td><td>7</td>
 
-
</tr>
 
-
<tr>
 
-
<td>TOTAL</td>
 
-
<td>12</td><td>12</td><td>12</td><td>12</td>
 
-
</tr>
 
-
</table>
 
-
:From left;
 
-
:
 
-
:
 
-
|}
 
-
{|align="justify"
 
-
|[[Image:Chiba-0904-2.JPG]]
 
-
|
 
-
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 
-
<tr>
 
-
<td width="257">Sample No</td>
 
-
<td>①</td><td>②</td><td>③</td><td>④</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Sample DNA</td>
 
-
<td>3</td><td>3</td><td>3</td><td>3</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Loading Dye</td>
 
-
<td>2</td><td>2</td><td>2</td><td>2</td>
 
-
</tr>
 
-
<tr>
 
-
<td>dH2O</td>
 
-
<td>7</td><td>7</td><td>7</td><td>7</td>
 
-
</tr>
 
-
<tr>
 
-
<td>TOTAL</td>
 
-
<td>12</td><td>12</td><td>12</td><td>12</td>
 
-
</tr>
 
-
</table>
 
-
:From left;
 
-
:
 
-
:
 
-
|}
 
-
 
-
{|align="justify"
 
-
|[[Image:Chiba-0904-3.JPG]]
 
-
|
 
-
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 
-
<tr>
 
-
<td width="257">Sample No</td>
 
-
<td>①</td><td>②</td><td>③</td><td>④</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Sample DNA</td>
 
-
<td>3</td><td>3</td><td>3</td><td>3</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Loading Dye</td>
 
-
<td>2</td><td>2</td><td>2</td><td>2</td>
 
-
</tr>
 
-
<tr>
 
-
<td>dH2O</td>
 
-
<td>7</td><td>7</td><td>7</td><td>7</td>
 
-
</tr>
 
-
<tr>
 
-
<td>TOTAL</td>
 
-
<td>12</td><td>12</td><td>12</td><td>12</td>
 
-
</tr>
 
-
</table>
 
-
:From left;
 
-
:
 
-
:
 
-
|}
 
-
 
-
===5 September, 2008===
 
-
 
-
===6 September, 2008===
 
-
 
-
===[[Team:Chiba/jk/β/weak 4|>next week]]===
 
-
 
-
{| style="color:white;background-color:Maroon" cellpadding="3" cellspacing="3" border="1" bordercolor="white" width="100%" align="center"
 
-
!align="center"|[[Team:Chiba/j|ホーム]]
 
-
!align="center"|[[Team:Chiba/Team/j|メンバー紹介]]
 
-
!align="center"|[[Team:Chiba/Project/j|プロジェクト紹介]]
 
-
!align="center"|[[Team:Chiba/Parts/j|Parts Submitted to the Registry]]
 
-
!align="center"|[[Team:Chiba/Modeling/j|モデリング]]
 
-
!align="center"|[[Team:Chiba/Internal|ノート]]
 
-
|}
 

Latest revision as of 07:03, 26 September 2008