Team:Chiba/jk/γ/week 2

From 2008.igem.org

(Difference between revisions)

Latest revision as of 05:50, 24 October 2008

>実験ログ

>出力班

WEEK 2

24,August 2008

Transformation

[http://partsregistry.org/Part:BBa_J63001 BBa_J63001](yeast optimized YFP)-->colony

Digestion

[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](1)①
[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2)②
Menbren Venus YFP③

Sample No.	①	②	③
DNA tamplate	3	3	3
Buffer3	1	1	1
dH₂O	6	6	6
EcoRⅠ	0.5	0.5	0.5
TOTAL	10	10	10

-->37℃ 30min

Sample No.	①(afetr digestion)	②(after digestion)	③(afetr digestion)
DNA tamplate	10	10	10
loading Dye	2	2	2
TOTAL	12	12	12

Sample No.	①	②	③
DNA tamplate	2	2	2
loading Dye	1	1	1
dH₂O	3	3	3
TOTAL	6	6	6

-->135V 30min

25,August,2008

PCR

[http://partsregistry.org/Part:BBa_E2030 BBa_E2030]①
[http://partsregistry.org/Part:BBa_J33202 BBa_J33202]②
BL21③
[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]④
[http://partsregistry.org/Part:BBa_I712019 BBa_I712019]⑤

>

Sample No.	①	②	③	④	⑤
DNA tamplate	1	1	1	1	1
FW primer	5(Venus_YFP_fwd)	5(LacZ_fwd)	5(LacZ_fwd)	5(rLUC_fwd)	5(fLuc_fwd)
VR primer	5(VR)	5(LacZ_rev)	5(LacZ_rev)	5(VR)	5(VR)
dNTPmix	10	10	10	10	10
Thermo pol buffer	10	10	10	10	10
DNA pol(VENT)	1	1	1	1	1
dH₂O	68	68	68	68	68
TOTAL	100	100	100	100	100

-->Gel Check

>

Sample No.	①	②	③	④	⑤
DNA tamplate	1	1	1	1	1
loading Dye	1	1	1	1	1
dH₂O	4	4	4	4	4
TOTAL	6	6	6	6	6

Mini prep

Venus
[http://partsregistry.org/Part:BBa_F2620 BBa_F2620]

Transformation

Venus
PUC19
pGFPUV
pGEX Venus YFP
Membren Venus YFP

PCR

[http://partsregistry.org/Part:BBa_J63001 BBa_J63001]①

Sample No.	①
DNA tamplate	1
FW primer(Venus)	5
VR primer	5
dNTPmix	10
Thermo pol buffer	10
DNA pol(VENT)	1
dH₂O	68
TOTAL	100

26,August,2008

Gel Check

[http://partsregistry.org/Part:BBa_F2620 BBa_F2620]
[http://partsregistry.org/Part:BBa_F2620 BBa_F2620]
[http://partsregistry.org/Part:BBa_J63001 BBa_J63001]

>

Sample No.	①	②	③	④	⑤
DNA tamplate	1	1	1	1	1
Loading Dye	1	1	1	1	1
dH₂O	4	4	4	4	4
TOTAL	6	6	6	6	6

Digestion

vector:[http://partsregistry.org/Part:BBa_F2620 BBa_F2620]

Sample No.	[http://partsregistry.org/Part:BBa_F2620 BBa_F2620]
DNA tamplate	100
SpeⅠ	2
PstⅠ	2
BSA(x10)	13
Buffer2	13
TOTAL	130

insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008],[http://partsregistry.org/Part:BBa_E2030 BBa_E2030]

Sample No.	[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]	[http://partsregistry.org/Part:BBa_E2030 BBa_E2030]
DNA tamplate	30	30
XbaⅠ	1	1
PstⅠ	1	1
DpnⅠ	1	1
Buffer3	4	4
BSA(x10)	4	4
TOTAL	40	40

-->37℃　3hour
-->SAP
-->37℃ 30min
-->65℃ 15min
-->Zymo Clean

Ligation

vector:[http://partsregistry.org/Part:BBa_F2620 BBa_F2620] insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]①
vector:[http://partsregistry.org/Part:BBa_F2620 BBa_F2620] insert:[http://partsregistry.org/Part:BBa_E2030 BBa_E2030]②
Negative control:[http://partsregistry.org/Part:BBa_F2620 BBa_F2620]③

Sample No.	①	②	③
vector	5	5	5
inser	10	7.5	0
Ligase Buffer	4	4	4
Ligase	1	1	1
dH₂O	0	2.5	10
TOTAL	20	20	20

-->RT 2hour

-->Transformation(XL10G)

-->37℃ over night

-->colony PCR

-->Mini prep

28,August,2008

Time Responce(liquid)

29,August,2008

TIME RESPONCE(solid)

30,August,2008

Transformation

mcherry

PCR

[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](rLuc)

Sample No.	[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]
DNA tamplate	1
rLuc_fwd	2.5
Rev primer	2.5
Thermo pol Buffer	5
dNTPmix	5
Vent pol	0.5
dH₂O	34
TOTAL	50

>Last week

>Next week

ホーム	メンバー紹介	プロジェクト紹介	Parts Submitted to the Registry	モデリング	ノート

@@ Line 1: / Line 1: @@
+[[Team:Chiba/jk|>実験ログ]]
+[[Team:Chiba/jk/γ|>出力班]]
 == WEEK 2 ==
 == 24,August 2008 ==
+[[Team:Chiba/protocol/transformation|Transformation]]
+*[http://partsregistry.org/Part:BBa_J63001 BBa_J63001](yeast optimized YFP)-->colony
+[[Team:Chiba/protocol/digestion|Digestion]]
+*[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](1)①
+*[http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2)②
+*Menbren Venus YFP③
-<br>TRANSFORMATION
-<br>BBa_J63001(yeast optimized YFP)-->colony
-<br>Digestion
-<br>BBa_F2620(1)①
-<br>BBa_F2620(2)②
-<br>Menbren Venus YFP③
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">Sample No</td>
+<td width="257">Sample No.</td>
 <td>①</td><td>②</td><td>③</td>
 </tr>
@@ Line 24: / Line 28: @@
 </tr>
 <tr>
-<td>dH2O</td>
+<td>dH<sub>2</sub>O</td>
 <td>6</td><td>6</td><td>6</td>
 </tr>
@@ Line 36: / Line 40: @@
 </tr>
 </table>
-<br>->37℃ 30min
+-->37℃ 30min
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">Sample No</td>
+<td width="257">Sample No.</td>
 <td>①(afetr digestion)</td><td>②(after digestion)</td><td>③(afetr digestion)</td>
 </tr>
@@ Line 55: / Line 63: @@
 </tr>
 </table>
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">Sample No</td>
+<td width="257">Sample No.</td>
 <td>①</td><td>②</td><td>③</td>
 </tr>
@@ Line 69: / Line 79: @@
 </tr>
 <tr>
-<td>dH2O</td>
+<td>dH<sub>2</sub>O</td>
 <td>3</td><td>3</td><td>3</td>
 </tr>
@@ Line 77: / Line 87: @@
 </tr>
 </table>
-<br>->135V 30min
+-->135V 30min
 == 25,August,2008 ==
-<br>PCR
+[[Team:Chiba/protocol/PCR|PCR]]
-<br>BBa_E2030①
+*[http://partsregistry.org/Part:BBa_E2030 BBa_E2030]①
-<br>BBa_J33202②
+*[http://partsregistry.org/Part:BBa_J33202 BBa_J33202]②
-<br>BL21③
+*BL21③
-<br>BBa_J52008④
+*[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]④
-<br>BBa_I712019⑤
+*[http://partsregistry.org/Part:BBa_I712019 BBa_I712019]⑤
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">Sample No</td>
+<td width="257">Sample No.</td>
 <td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td>
 </tr>
@@ Line 110: / Line 122: @@
 </tr>
 <tr>
-<td>thermo pol buffer</td>
+<td>Thermo pol buffer</td>
 <td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
 </tr>
@@ Line 118: / Line 130: @@
 </tr>
 <tr>
-<td>dH2O</td>
+<td>dH<sub>2</sub>O</td>
 <td>68</td><td>68</td><td>68</td><td>68</td><td>68</td>
 </tr>
@@ Line 126: / Line 138: @@
 </tr>
 </table>
-<br>->Gel
+-->[[Team:Chiba/protocol/gelcheck|Gel Check]]
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">Sample No</td>
+<td width="257">Sample No.</td>
 <td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td>
 </tr>
@@ Line 141: / Line 156: @@
 </tr>
 <tr>
-<td>dH2O</td>
+<td>dH<sub>2</sub>O</td>
 <td>4</td><td>4</td><td>4</td><td>4</td><td>4</td>
 </tr>
@@ Line 150: / Line 165: @@
 </table>
-<br>MINI prep
+[[Team:Chiba/protocol/DNA_Purification/sigma|Mini prep]]
-<br>Venus
+*Venus
-<br>BBa_F2620
+*[http://partsregistry.org/Part:BBa_F2620 BBa_F2620]
-<br>TRANSFORMATRION
-<br>Venus
-<br>PUC19
+[[Team:Chiba/protocol/transformation|Transformation]]
-<br>pGFPUV
+*Venus
-<br>pGEX Venus YFP
+*PUC19
-<br>Membren Venus YFP
+*pGFPUV
-<br>PCR
+*pGEX Venus YFP
-<br>BBa_J63001①
+*Membren Venus YFP
+[[Team:Chiba/protocol/PCR|PCR]]
+*[http://partsregistry.org/Part:BBa_J63001 BBa_J63001]①
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">Sample No</td>
+<td width="257">Sample No.</td>
 <td>①</td>
 </tr>
@@ Line 183: / Line 200: @@
 </tr>
 <tr>
-<td>thermo pol buffer</td>
+<td>Thermo pol buffer</td>
 <td>10</td>
 </tr>
@@ Line 191: / Line 208: @@
 </tr>
 <tr>
-<td>dH2O</td>
+<td>dH<sub>2</sub>O</td>
 <td>68</td>
 </tr>
@@ Line 199: / Line 216: @@
 </tr>
 </table>
 == 26,August,2008 ==
-<br>Gel check
+[[Team:Chiba/protocol/gelcheck|Gel Check]]
-<br>BBa_F2620
+*[http://partsregistry.org/Part:BBa_F2620 BBa_F2620]
-<br>BBa_F2620
+*[http://partsregistry.org/Part:BBa_F2620 BBa_F2620]
-<br>BBa_J63001
+*[http://partsregistry.org/Part:BBa_J63001 BBa_J63001]
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">Sample No</td>
+<td width="257">Sample No.</td>
 <td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td>
 </tr>
@@ Line 222: / Line 237: @@
 </tr>
 <tr>
-<td>dH2O</td>
+<td>dH<sub>2</sub>O</td>
 <td>4</td><td>4</td><td>4</td><td>4</td><td>4</td>
 </tr>
@@ Line 231: / Line 246: @@
 </table>
-<br>Digestion
+[[Team:Chiba/protocol/digestion|Digestion]]
-<br>vector:BBa_F2620
+*vector:[http://partsregistry.org/Part:BBa_F2620 BBa_F2620]
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">Sample No</td>
+<td width="257">Sample No.</td>
-<td>BBa_F2620</td>
+<td>[http://partsregistry.org/Part:BBa_F2620 BBa_F2620]</td>
 </tr>
 <tr>
@@ Line 258: / Line 274: @@
 <td>13</td>
 </tr>
+<tr>
 <td>TOTAL</td>
 <td>130</td>
 </tr>
 </table>
-<br>insert:BBa_J52008,BBa_E2030
+*insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008],[http://partsregistry.org/Part:BBa_E2030 BBa_E2030]
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">Sample No</td>
+<td width="257">Sample No.</td>
-<td>J52008</td><td>E2030</td>
+<td>[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]</td><td>[http://partsregistry.org/Part:BBa_E2030 BBa_E2030]</td>
 </tr>
 <tr>
@@ Line 292: / Line 311: @@
 <td>4</td><td>4</td>
 </tr>
+<tr>
 <td>TOTAL</td>
 <td>40</td><td>40</td>
 </tr>
 </table>
-<br>->37℃　3hour
+<br>-->37℃　3hour
-<br>->SAP
+<br>-->SAP
-<br>->37℃ 30min
+<br>-->37℃ 30min
-<br>->65℃ 15min
+<br>-->65℃ 15min
-<br>->Zymo Clean
+<br>-->[[Team:Chiba/protocol/DNA_Purification/zymocleam|Zymo Clean]]
-<br>Ligation
-<br>vector:BBa_F2620 insert:BBa_J52008①
+[[Team:Chiba/protocol/ligation/ligation|Ligation]]
-<br>vector:BBa_F2620 insert:BBa_E2030②
-<br>Negative control:BBa_F2620③
+*vector:[http://partsregistry.org/Part:BBa_F2620 BBa_F2620] insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]①
+*vector:[http://partsregistry.org/Part:BBa_F2620 BBa_F2620] insert:[http://partsregistry.org/Part:BBa_E2030 BBa_E2030]②
+*Negative control:[http://partsregistry.org/Part:BBa_F2620 BBa_F2620]③
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">Sample No</td>
+<td width="257">Sample No.</td>
 <td>①</td><td>②</td><td>③</td>
 </tr>
@@ Line 327: / Line 350: @@
 </tr>
 <tr>
-<td>dH2O</td>
+<td>dH<sub>2</sub>O</td>
 <td>0</td><td>2.5</td><td>10</td>
 </tr>
@@ Line 335: / Line 358: @@
 </tr>
 </table>
-<br>->RT 2hour
-<br>->TRANSFORMATION XL10G
-<br>->37℃ over night
-<br>->colony PCR
-<br>->MINI PREP
+-->RT 2hour
+-->[[Team:Chiba/protocol/transformation|Transformation]](XL10G)
+-->37℃ over night
+-->colony PCR
+-->[[Team:Chiba/protocol/DNA_Purification/sigma|Mini prep]]
 == 28,August,2008 ==
-<br>TIME RESPONCE(liquid)
+[[Team:Chiba/jk/γ/Trl|Time Responce(liquid)]]
@@ Line 350: / Line 378: @@
 == 29,August,2008 ==
-<br>TIME RESPONCE(solid)
+[[Team:Chiba/jk/γ/trs|TIME RESPONCE(solid)]]
 == 30,August,2008 ==
-<br>TRANSFORMATION
+[[Team:Chiba/protocol/transformation|Transformation]]
-<br>mcherry
-<br>PCR
+*mcherry
-<br>BBa_J52008(rLuc)
+[[Team:Chiba/protocol/PCR|PCR]]
+*[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](rLuc)
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">Sample No</td>
+<td width="257">Sample No.</td>
-<td>BBa_J52008</td>
+<td>[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]</td>
 </tr>
 <tr>
@@ Line 373: / Line 406: @@
 </tr>
 <tr>
-<td>VR</td>
+<td>Rev primer</td>
 <td>2.5</td>
 </tr>
 <tr>
-<td>thermo pol Buffer</td>
+<td>Thermo pol Buffer</td>
 <td>5</td>
 </tr>
 <tr>
-<td>dNTP</td>
+<td>dNTPmix</td>
 <td>5</td>
 </tr>
@@ Line 389: / Line 422: @@
 </tr>
 <tr>
-<td>dH2O</td>
+<td>dH<sub>2</sub>O</td>
 <td>34</td>
 </tr>
+<tr>
 <td>TOTAL</td>
 <td>50</td>
@@ Line 400: / Line 434: @@
-[[Media:Team:Chiba/jk/γ/week 3]]
+[[Team:Chiba/jk/γ/week 1|>Last week]]
+[[Team:Chiba/jk/γ/week 3|>Next week]]
 {| style="color:white;background-color:Maroon" cellpadding="3" cellspacing="3" border="1" bordercolor="white" width="100%" align="center"

Team:Chiba/jk/γ/week 2

From 2008.igem.org

Latest revision as of 05:50, 24 October 2008

Contents

WEEK 2

24,August 2008

25,August,2008

26,August,2008

28,August,2008

29,August,2008

30,August,2008