Team:Chiba/jk/γ/week 4

From 2008.igem.org

実験ログ
出力班

Contents

Week 4

7,September,2008

  • Cloning

Digestion

  • vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)① 75ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)② 33.6ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)③ 33.6ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160](RBS+cinR)④ 38.4ng/μl
  • vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](pLac)⑤ 100ng/μl
  • insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]⑥ 33.6ng/μl
Sample No.
DNA(digested sample) 505454451454
SpeⅠ 100010
PstⅠ 0.50.50.50.50.50.5
XbaⅠ 00.50.50.500.5
BSA(x10) 0.677627
Buffer2 6---2-
Buffer3 -776-7
dH2O 1.91120.51
TOTAL 607070602070

-->37℃ 3hour

-->Gel Extraction

Sample No.
DNA(digested sample) 6101010410
Loading Dye 222212
dH2O 4---12
TOTAL 12121212612
well 1077657

-->DNA purification (①-④,⑥:7μl ⑤:6μl)


8,Septmeber,2008

SAP

  • [http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)①
  • [http://partsregistry.org/Part:BBa_R0010 BBa_R0010](pLac)⑤
Sample No.
DNA(digested sample) 76
SAP/td> 22
SAP Buffer 33
TOTAL 3030
TOTAL 3030

-->37℃ 1hour

-->65℃ 30min

-->DNA purification

-->21μl 6μl

-->Gel check

Sample No. each
DNA(digested sample) 1
Loading Dye 1
dH2O 4
TOTAL 6
  • vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)① 75ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)② 33.6ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)③ 33.6ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160](RBS+cinR)④ 38.4ng/μl
  • vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](pLac)⑤ 100ng/μl
  • insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](RBS+rLuc)⑥ 33.6ng/μl
Sample No. ①&②①&③①&④⑤&⑥
DNA(vector) 333352.1
DNA(insert) 333-3.1-
Ligase 111111
Ligase Buffer(x5) 222222
dH2O 11148.94
TOTAL 101010102010


9,September,2008

10,September,2008

TIME Responce(liquid)

TIME Responce(solid)

Colony PCR

-->Gel check

-->Mini prep

11,September,2008

12,September,2008

13,September,2008


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