Team:Chiba/protocol/ligation/dephosphorylation

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>Protcol

dephosphorylation

SAP: Alkaline Phosphatase (Shrimp)

  • Mix 
DNA fragment 1~10 pmol
Shrimp Alkaline Phosphatase (1~5 μ l) 1~5 U
10X SAP Buffer 5 μ l
Sterilized distilled water up to 50 μ l
  1. Incubate at 37℃ for 15~30 min.
  2. Incubate at 65℃ for 15 min. (for inactivation by heat treatment)
  3. Purify the DNA with Zymo DNA Cleam&Concentrator Kit

READ:Zymo cleam

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