Team:Davidson-Missouri Western/Online Tools that Support Design of New Biobrick Parts

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Revision as of 16:57, 29 October 2008 by Macampbell (Talk | contribs)

During the summer, we realize two substantial shortcomings in the iGEM world.

1. How do you choose the right percent gel for separating any two DNA bands? Rather than guessing, there should be a more reliable method, especially since so many iGEM students are new to the lab. Therefore, we produced a Gel Optimizer to help you choose the right percent agarose gel. When you submit the MW of your band, you will get the optimal percent agarose (between 2% and 0.4%) as well as a photo showing molecular weight markers at this percent gel and the equation that helped us determine the migration of fragments at the different gel concentration. Point8 gel.jpg506.jpg



2.

PCR Primer with BioBrick ends

<a href="http://gcat.davidson.edu/iGEM08/bbprimer.html" class="style12">

Frustrated with the carelessness of human error? This tool is designed to add complementary base pairs to any DNA sequence and design primers for PCR.

 

<a href="http://gcat.davidson.edu/iGEM08/gelwebsite/gelwebsite.html" class="style12">***Optimal Percent Agarose Gel***</a>

Ever wonder what would be the best concentration of agarose for your gels? This tool is designed to select the optimal percent agarose concentration based on the base pair length of dsDNA.