Team:ESBS-Strasbourg/25 July 2008

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Contents

DryLab

Modeling

WetLab

Manuel

  • PCR VFprimer lacI BB
  • Gelpurification
  • 2nd PCR
    • on product of first PCR with original primer
    • original primer directly on BB plasmid
  • Miniprep of J6300X (x=2,3,5,8)
  • Verification gel of miniprep

Paul
-Digestion of tetR, lacI, c1, in order to check if the biobricks are really in the plasmid. Digestion with EcoR1 and Spe1

-Digestion of the PCR products tetR, lexA, Gal4, in order to have them in a silver standard and to insert them into the vector p1010 1A2. In this way, this vector is also digested for future insertion of biobricks. Enzymes used: EcoR1 and Spe1.

General

  • Rechecked Primers to order (MM)

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