Team:ESBS-Strasbourg/Competent Bacteria

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Revision as of 12:19, 14 July 2008

Solutions

KCM 5x
100ml

0.5M KCl 3.7 g KCl 74,56 g/mol
0.15M CaCl2 2.2 g CaCl2 2H2O 147,02 g/mol
0.25M MgCl2 5 g MgCl2 6H2O 203,30 g/mol
autoclave

TSB
95 ml

LB about 80 ml
10% PEG (MW 3.350) 10 g
20 mM Mg++ 10 ml of 200mM
autoclave
5% DMSO 5 ml


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