Team:ESBS-Strasbourg/Protocols

From 2008.igem.org

(Difference between revisions)
(E.Coli)
(Solutions and buffers)
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==Solutions and buffers==
==Solutions and buffers==
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'''SOB''' for the preparation of competent cells [http://openwetware.org/wiki/SOB SOB] <br>
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*'''SOB''' for the preparation of competent cells [http://openwetware.org/wiki/SOB SOB] <br>
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Revision as of 22:31, 14 July 2008

E.Coli

  • Preparation of TOP10 chemically competent cells [http://openwetware.org/wiki/TOP10_chemically_competent_cells Igem Standard Protocol]
  • Transformation of competent cells (Mariels/Barbaras Protocol) Transformation


Cloning

Solutions and buffers

  • SOB for the preparation of competent cells [http://openwetware.org/wiki/SOB SOB]