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Team:ESBS-Strasbourg/TENS
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(Difference between revisions)
(New page: =TENS= Tris-HCl 10 mM pH8<br> EDTA 1 mM pH8<br> NaOH 0.1 M<br> SDS 0.5%<br> Make a Sterilfiltration of the solution or use steril stock solutions for its preparation '''For 40 mL of TEN...) |
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Tris-HCl 10 mM pH8<br> | Tris-HCl 10 mM pH8<br> | ||
EDTA 1 mM pH8<br> | EDTA 1 mM pH8<br> | ||
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Make a Sterilfiltration of the solution or use steril stock solutions for its preparation | Make a Sterilfiltration of the solution or use steril stock solutions for its preparation | ||
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'''For 40 mL of TENS:''' | '''For 40 mL of TENS:''' | ||
*0.8 mL of 0.5M Tris-HCL pH8 | *0.8 mL of 0.5M Tris-HCL pH8 | ||
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*0.4 mL of 10 M NaOH | *0.4 mL of 10 M NaOH | ||
*1 mL of 20% SDS | *1 mL of 20% SDS | ||
| - | *fill up to 40 mL with milliQ < | + | *fill up to 40 mL with milliQ water |
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| + | [[Team:ESBS-Strasbourg/Protocols|''Protocols'']] | ||
Latest revision as of 09:56, 17 July 2008
Tris-HCl 10 mM pH8
EDTA 1 mM pH8
NaOH 0.1 M
SDS 0.5%
Make a Sterilfiltration of the solution or use steril stock solutions for its preparation
For 40 mL of TENS:
- 0.8 mL of 0.5M Tris-HCL pH8
- 0.1 mL of 0.4M EDTA
- 0.4 mL of 10 M NaOH
- 1 mL of 20% SDS
- fill up to 40 mL with milliQ water
