Team:ETH Zurich/Modeling/Genome Static Analysis
From 2008.igem.org
Restriction Enzyme AnalysisThis section presents the computational investigation we performed in order to understand which restriction enzymes are optimal when used in our minimal genome approach, in order to find out which restriction enzymes cut the genome in fragments that most probably will lead to find the minimal genome in our reduction approach. Important is to note that this is a "statical" analysis, meaning that we do not include in the evaluation of the restriction enzyme optimality any prevision regarding to the effects its cutting patterns can have on cell physiology or cell system behaviour. We focus here only on the insights that can be obtained using three kinds of "statical" information:
We addressed questions regarding the cell system response after genome reduction using more advanced modelling techniques (a genome scale model) in the Genome Scale Analysis section. Nevertheless, relying only on static analysis it is possible to ask (and answer) the following questions:
Availabel restricion enzymesTable with restriction enzymes analysis. |