Team:ETH Zurich/Modeling/Genome Static Analysis/RestrictionTable

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Revision as of 15:02, 25 October 2008 by Luca.Gerosa (Talk | contribs)


The table below summarizes the data obtained after simulationing the digestion of the E.Coli K12 MG1655 genome with the complete set of 713 restriction enzymes present in the [http://rebase.neb.com/rebase/rebase.html REBASE database]. For each restriction enzymes we simulated the fragments of genome obtained after digestion. Using the available annotation of the genome (that is, position of the genes on the chromosome), we calculated the number of genes disrupted if each fragment was to be removed from the genome. We calculated statistical measures of the restriction enzyme effects, such as average size of the fragments and its variance, and the average number of (all and only essential) genes and its variance.

Note 1: several restriction enzymes cut using the same pattern, they have been clustered on the same line of the table. The clusters are 262.
Note 2: the patterns are expressed according to the MATLAB regular expression rules for nucleotides. Apart from the obvious meaning of a,t,c,g there are the special characters such as R for Purine([AG]), Y for Pyrimidine ([TC]), K for Keto ([GT]), M for Amino [AC]). For the full detail look at the [http://www.mathworks.com/access/helpdesk/help/toolbox/bioinfo/index.html?/access/helpdesk/help/toolbox/bioinfo/ref/restrict.html&http://www.google.com/search?hl=en&q=matlab+regexp+nucleotides&btnG=Search MATLAB help page].

Statistic on genome fragments from restriction enzyme digestion
Restriction Enzyme(s) Recognition Site Number of fragments Average size of fragments (Kbp) Variance size of fragments (Kbp) Average number of genes per fragments Variance number of genes per fragments Percentage of fragments with one or more essential genes
FseI RigI GGCCGGCC 4 1161583 1.388453e+012 935 1225974 1
AbsI CCTCGAGG 8 5.807915e+005 2.485291e+011 4.285000e+002 1.724534e+005 1
AspA2I AvrII BlnI XmaJI CCTAGG 16 2.903958e+005 1.270844e+011 2.673750e+002 1.135188e+005 6.250000e-001
CciNI NotI GCGGCCGC 23 2.020144e+005 3.296516e+010 1.746522e+002 2.725442e+004 7.391304e-001
MreI Sse232I CGCCGGCG 30 1.548777e+005 1.988095e+010 1.289000e+002 1.419651e+004 7.000000e-001
SfiI GGCCNNNNNGGCC 31 1.498817e+005 1.719847e+010 1.281290e+002 1.367005e+004 7.419355e-001
SanDI GGGWCCC 33 1.407979e+005 1.633491e+010 1.313030e+002 1.409122e+004 7.575758e-001
SgrDI CGTCGACG 36 1.290648e+005 1.912706e+010 1.158889e+002 1.450587e+004 6.388889e-001
XbaI TCTAGA 40 1.161583e+005 1.261032e+010 1.070750e+002 1.135899e+004 6.500000e-001
SrfI GCCCGGGC 43 1.080542e+005 6.721085e+009 9.620930e+001 4.987455e+003 6.976744e-001
Sse8647I aggwcct 66 7.039897e+004 5.111447e+009 6.515152e+001 4.528100e+003 5.757576e-001
ApaI Bsp120I PspOMI GGGCCC 67 6.934824e+004 4.844790e+009 6.386567e+001 4.451603e+003 5.522388e-001
SbfI SdaI Sse8387I CCTGCAGG 68 6.832841e+004 4.351616e+009 6.173529e+001 3.424944e+003 5.441176e-001
AhlI BcuI SpeI ACTAGT 86 5.402712e+004 4.732091e+009 5.041860e+001 3.912482e+003 4.069767e-001
MssI PmeI GTTTAAAC 87 5.340611e+004 2.475081e+009 4.841379e+001 2.155548e+003 4.482759e-001
BbeI DinI EgeI EheI KasI Mly113I NarI SfoI GGCGCC 99 4.693265e+004 2.444902e+009 4.311111e+001 2.108875e+003 4.141414e-001
PspXI VCTCGAGB 110 4.223938e+004 2.634945e+009 3.784545e+001 2.109563e+003 4.000000e-001
SmiI SwaI ATTTAAAT 117 3.971224e+004 1.557045e+009 3.688889e+001 1.288738e+003 4.017094e-001
BsaI Bso31I BspTNI Eco31I GGTCTC 119 3.904481e+004 2.461282e+009 36 2.099407e+003 3.445378e-001
BbvCI CCTCAGC 129 3.601808e+004 2.189274e+009 3.353488e+001 1.831407e+003 3.333333e-001
UbaF12I ctacnnngtc 140 3.318809e+004 8.916053e+008 3.092857e+001 8.460956e+002 4.000000e-001
AcvI BbrPI Eco72I PmaCI PmlI PspCI CACGTG 143 3.249183e+004 1.665365e+009 3.002098e+001 1.466401e+003 3.496503e-001
PacI TTAATTAA 143 3.249183e+004 1.107201e+009 3.002098e+001 8.897390e+002 3.216783e-001
Ecl136II EcoICRI Psp124BI SacI SstI GAGCTC 151 3.077041e+004 9.945293e+008 2.866887e+001 8.549030e+002 3.841060e-001
AsuNHI BmtI BspOI NheI GCTAGC 156 2.978418e+004 7.909314e+008 2.779487e+001 6.727835e+002 3.333333e-001
MauBI cgcgcgcg 160 2.903958e+004 9.985228e+008 2.697500e+001 9.167038e+002 3.562500e-001
AscI PalAI SgsI GGCGCGCC 165 2.815959e+004 8.653289e+008 2.601818e+001 7.766155e+002 2.969697e-001
PaeR7I SciI Sfr274I SlaI StrI TliI XhoI CTCGAG 178 2.610299e+004 5.691406e+008 2.412360e+001 4.729564e+002 3.202247e-001
AsiSI RgaI SgfI GCGATCGC 222 2.092942e+004 3.532848e+008 1.950000e+001 3.320973e+002 2.702703e-001
AxyI Bse21I Bsu36I Eco81I SauI CCTNAGG 257 1.807911e+004 3.219393e+008 1.687160e+001 2.831905e+002 2.529183e-001
PpuMI Psp5II PspPPI RGGWCCY 257 1.807911e+004 4.225263e+008 1.687160e+001 3.857842e+002 2.334630e-001
BseX3I BstZI EagI EclXI Eco52I XmaIII CGGCCG 282 1.647635e+004 3.461436e+008 1.537234e+001 3.156296e+002 2.304965e-001
MroNI NaeI NgoMIV PdiI GCCGGC 302 1.538521e+004 2.867138e+008 1.435099e+001 2.609395e+002 2.152318e-001
UbaF9I tacnnnnnrtgt 302 1.538521e+004 2.427370e+008 1.431126e+001 2.287533e+002 2.119205e-001
BspQI LguI PciSI SapI GCTCTTC 334 1.391117e+004 2.753008e+008 1.285928e+001 2.426378e+002 1.826347e-001
PasI CCCWGGG 336 1.382837e+004 1.760959e+008 1.287202e+001 1.718552e+002 1.785714e-001
PpiI GAACNNNNNCTC 342 1.358577e+004 5.363972e+008 1.226608e+001 4.301665e+002 1.491228e-001
BauI BsiI BssSI Bst2BI CACGAG 358 1.297858e+004 2.254207e+008 1.206145e+001 1.996097e+002 1.843575e-001
UbaF13I gagnnnnnnctgg 362 1.283517e+004 1.914252e+008 1.196961e+001 1.724340e+002 1.629834e-001
BseRI GAGGAG 388 1.197508e+004 1.778986e+008 1.114691e+001 1.500585e+002 1.675258e-001
CpoI CspI RsrII Rsr2I CGGWCCG 389 1.194430e+004 1.654756e+008 1.111825e+001 1.481045e+002 1.670951e-001
BssNAI Bst1107I BstZ17I SnaI GTATAC 391 1.188320e+004 1.314576e+008 1.107417e+001 1.120073e+002 1.815857e-001
FspAI RTGCGCAY 397 1.170361e+004 1.372206e+008 1.091184e+001 1.236968e+002 1.662469e-001
AssI BmcAI ScaI ZrmI AGTACT 400 1.161583e+004 1.424631e+008 1.071000e+001 1.225924e+002 1.550000e-001
Cfr9I SmaI TspMI XmaI XmaCI CCCGGG 429 1.083061e+004 1.237159e+008 1.008159e+001 1.070985e+002 1.794872e-001
PsrI GAACNNNNNNTAC 430 1.080542e+004 3.827740e+008 1.007674e+001 3.428822e+002 1.325581e-001
AloI GAACNNNNNNTCC 458 1.014483e+004 3.571443e+008 9.447598e+000 3.339721e+002 1.179039e-001
AjuI GAANNNNNNNTTGG 466 9.970670e+003 2.914028e+008 9.261803e+000 2.620517e+002 1.201717e-001
AlwFI gaaaynnnnnrtg 467 9.949319e+003 1.144785e+008 9.259101e+000 9.897350e+001 1.456103e-001
BspLU11I PciI PscI ACATGT 475 9.781752e+003 1.012818e+008 9.120000e+000 8.970498e+001 1.452632e-001
TssI gagnnnctc 485 9.580066e+003 8.958870e+007 8.927835e+000 8.277371e+001 1.505155e-001
Bce83I BpuEI cttgag 487 9.540723e+003 9.609306e+007 8.878850e+000 8.443591e+001 1.519507e-001
BamHI GGATCC 494 9.405530e+003 8.826372e+007 8.761134e+000 7.981301e+001 1.477733e-001
Pfl1108I tcgtag 497 9.348757e+003 1.009106e+008 8.696177e+000 9.323613e+001 1.488934e-001
AhdI AspEI BmeRI DriI Eam1105I EclHKI GACNNNNNGTC 502 9.255641e+003 9.869322e+007 8.625498e+000 9.207504e+001 1.553785e-001
DraII EcoO109I PssI RGGNCCY 507 9.164363e+003 1.077677e+008 8.552268e+000 9.696712e+001 1.420118e-001
Acc65I Asp718I KpnI GGTACC 517 8.987103e+003 9.802089e+007 8.382979e+000 9.524064e+001 1.566731e-001
BsmBI Esp3I CGTCTC 530 8.766664e+003 8.445298e+007 8.173585e+000 7.845752e+001 1.245283e-001
BanII Eco24I EcoT38I FriOI HgiJII GRGCYC 531 8.750154e+003 1.102563e+008 8.152542e+000 1.005937e+002 1.450094e-001
SalI GTCGAC 544 8.541051e+003 7.756234e+007 7.961397e+000 7.273331e+001 1.323529e-001
BstSNI Eco105I SnaBI TACGTA 545 8.525380e+003 7.290952e+007 7.955963e+000 6.732526e+001 1.247706e-001
SgrAI CRCCGGYG 547 8.494208e+003 8.070398e+007 7.915905e+000 7.989401e+001 1.334552e-001
Bpu10I CCTNAGC 553 8.402047e+003 7.139938e+007 7.822785e+000 6.174028e+001 1.374322e-001
BstENI EcoNI XagI CCTNNNNNAGG 553 8.402047e+003 7.377756e+007 7.822785e+000 6.588883e+001 1.301989e-001
AflII BfrI BspTI Bst98I MspCI Vha464I CTTAAG 557 8.341709e+003 7.642692e+007 7.779174e+000 7.242057e+001 1.328546e-001
HindIII AAGCTT 561 8.282232e+003 9.146291e+007 7.709447e+000 8.280650e+001 1.229947e-001
AarI CACCTGC 568 8.180162e+003 7.270785e+007 7.609155e+000 7.131082e+001 1.250000e-001
Bsp1407I BsrGI BstAUI SspBI TGTACA 569 8.165786e+003 6.052577e+007 7.597540e+000 5.689232e+001 1.370826e-001
Alw44I ApaLI VneI GTGCAC 575 8.080577e+003 6.496629e+007 7.539130e+000 6.295970e+001 1.304348e-001
CstMI aaggag 577 8.052568e+003 5.843356e+007 7.511265e+000 5.351767e+001 1.265165e-001
AspI PflFI PsyI Tth111I GACNNNGTC 581 7.997129e+003 6.373313e+007 7.462995e+000 6.003527e+001 1.290878e-001
BbuI PaeI SphI GCATGC 591 7.861814e+003 7.342241e+007 7.335025e+000 6.541977e+001 1.184433e-001
BsiWI Pfl23II PspLI SplI CGTACG 592 7.848534e+003 6.761762e+007 7.312500e+000 6.442164e+001 1.334459e-001
AatI Eco147I PceI SseBI StuI AGGCCT 604 7.692603e+003 7.400240e+007 7.167219e+000 7.203998e+001 1.192053e-001
CspCI CAANNNNNGTGG 606 7.667215e+003 1.812084e+008 7.155116e+000 1.552057e+002 1.072607e-001
BmgI gkgccc 617 7.530522e+003 6.062109e+007 7.006483e+000 5.899022e+001 1.280389e-001
Bsp19I NcoI CCATGG 619 7.506191e+003 5.573513e+007 6.972536e+000 5.226947e+001 1.211632e-001
BalI MlsI MluNI MscI Msp20I TGGCCA 636 7.305553e+003 5.185561e+007 6.812893e+000 4.953816e+001 1.226415e-001
EcoRI GAATTC 649 7.159217e+003 5.447496e+007 6.671803e+000 5.029798e+001 1.186441e-001
Cfr42I KspI SacII Sfr303I SgrBI SstII CCGCGG 664 6.997488e+003 4.434429e+007 6.524096e+000 4.313366e+001 1.234940e-001
BoxI BstPAI PshAI GACNNNNGTC 674 6.893668e+003 4.704915e+007 6.408012e+000 4.697592e+001 1.201780e-001
FauNDI NdeI CATATG 682 6.812804e+003 4.408884e+007 6.338710e+000 4.121991e+001 1.129032e-001
BglII AGATCT 704 6.599903e+003 4.166171e+007 6.147727e+000 4.007203e+001 1.036932e-001
AatII ZraI GACGTC 713 6.516595e+003 4.402359e+007 6.077139e+000 4.286623e+001 1.136045e-001
AasI DrdI DseDI GACNNNNNNGTC 732 6.347448e+003 4.771756e+007 5.922131e+000 4.714988e+001 1.106557e-001
RleAI cccaca 737 6.304385e+003 4.379164e+007 5.879240e+000 4.430741e+001 9.769335e-002
AjiI BmgBI BtrI CACGTC 750 6.195109e+003 3.923853e+007 5.780000e+000 3.753899e+001 1.080000e-001
BciVI BfuI GTATCC 750 6.195109e+003 3.753824e+007 5.780000e+000 3.443084e+001 1.200000e-001
BplI GAGNNNNNCTC 766 6.065708e+003 1.331965e+008 5.652742e+000 1.218034e+002 8.877285e-002
AfeI Aor51HI Eco47III AGCGCT 778 5.972149e+003 4.358465e+007 5.568123e+000 4.418390e+001 1.041131e-001
BaeI ACNNNNGTAYC 778 5.972149e+003 1.137306e+008 5.548843e+000 1.008863e+002 8.868895e-002
AsuII Bpu14I Bsp119I BspT104I BstBI Csp45I NspV SfuI TTCGAA 799 5.815184e+003 4.529479e+007 5.425532e+000 4.161068e+001 1.051314e-001
MabI SexAI ACCWGGT 807 5.757537e+003 3.149664e+007 5.371747e+000 2.968545e+001 1.040892e-001
AdeI DraIII CACNNNGTG 838 5.544549e+003 3.006020e+007 5.161098e+000 2.996326e+001 9.785203e-002
Bbr7I BbsI BbvII BpiI BpuAI BstV2I gaagac 840 5.531348e+003 3.739786e+007 5.160714e+000 3.559631e+001 1.047619e-001
AvaIII EcoT22I Mph1103I NsiI Ppu10I Zsp2I atgcat 844 5.505133e+003 3.383794e+007 5.136256e+000 3.142744e+001 8.293839e-002
BlpI Bpu1102I Bsp1720I CelII EspI GCTNAGC 864 5.377699e+003 3.319901e+007 5.015046e+000 3.153280e+001 1.053241e-001
PsiI TTATAA 871 5.334480e+003 3.987655e+007 4.978186e+000 3.585355e+001 8.151550e-002
BfaI FspBI MaeI XspI CTAG 886 5.244167e+003 4.230087e+007 4.893905e+000 3.747800e+001 8.803612e-002
AleI OliI CACNNNNGTG 892 5.208892e+003 2.691152e+007 4.859865e+000 2.684117e+001 8.856502e-002
AccIII Aor13HI BlfI BseAI Bsp13I BspEI BspMII Kpn2I MroI TCCGGA 918 5.061364e+003 3.062768e+007 4.712418e+000 2.924981e+001 8.714597e-002
BsaXI ACNNNNNCTCC 926 5.017637e+003 8.028972e+007 4.678186e+000 6.949849e+001 7.991361e-002
BspMAI PstI CTGCAG 956 4.860180e+003 3.293598e+007 4.529289e+000 2.898448e+001 9.205021e-002
AccBSI BsrBI MbiI CCGCTC 981 4.736322e+003 2.652703e+007 4.417941e+000 2.645168e+001 7.645260e-002
MfeI MunI CAATTG 993 4.679086e+003 2.233435e+007 4.366566e+000 2.162759e+001 7.854985e-002
BfiI BmrI BmuI ACTGGG 1010 4.600329e+003 2.712749e+007 4.286139e+000 2.642845e+001 8.514851e-002
DrdII gaacca 1036 4.484876e+003 2.426710e+007 4.183398e+000 2.387744e+001 7.915058e-002
PfoI TCCNGGA 1036 4.484876e+003 1.797340e+007 4.169884e+000 1.812957e+001 8.880309e-002
BspGI ctggac 1045 4.446251e+003 2.228959e+007 4.142584e+000 2.129095e+001 8.133971e-002
NmeAIII GCCGAG 1047 4.437757e+003 2.560076e+007 4.140401e+000 2.644968e+001 7.831901e-002
BssT1I Eco130I EcoT14I ErhI StyI CCWWGG 1083 4.290242e+003 2.254792e+007 3.992613e+000 2.147869e+001 7.848569e-002
UbaPI cgaacg 1117 4.159653e+003 1.900650e+007 3.880931e+000 1.932004e+001 7.520143e-002
CjuII caynnnnnctc 1118 4.155932e+003 1.842937e+007 3.869410e+000 1.646458e+001 7.155635e-002
MmeI TCCRAC 1119 4.152218e+003 1.821435e+007 3.874888e+000 1.885016e+001 7.327971e-002
BspHI PagI RcaI TCATGA 1159 4.008915e+003 1.877300e+007 3.738568e+000 1.828652e+001 7.247627e-002
BsbI caacac 1179 3.940909e+003 1.510531e+007 3.667515e+000 1.472977e+001 7.633588e-002
Ama87I AvaI BmeT110I BsiHKCI BsoBI Eco88I Nli3877I CYCGRG 1251 3.714094e+003 1.459236e+007 3.463629e+000 1.474328e+001 7.354117e-002
BsaMI BsmI Mva1269I PctI GAATGC 1317 3.527967e+003 1.298815e+007 3.287775e+000 1.231302e+001 6.529992e-002
MluI ACGCGT 1322 3.514623e+003 1.261258e+007 3.275340e+000 1.379090e+001 6.807867e-002
BpvUI MvrI Ple19I PvuI CGATCG 1344 3.457092e+003 1.374162e+007 3.223214e+000 1.520107e+001 6.919643e-002
GdiII cggccr 1369 3.393961e+003 1.498846e+007 3.166545e+000 1.520616e+001 6.793280e-002
BslFI BsmFI FaqI FinI GGGAC 1377 3.374243e+003 1.520394e+007 3.148148e+000 1.517571e+001 6.390704e-002
BstEII BstPI Eco91I EcoO65I PspEI GGTNACC 1382 3.362035e+003 1.183829e+007 3.136035e+000 1.199741e+001 6.874096e-002
BpmI GsuI CTGGAG 1389 3.345091e+003 1.411505e+007 3.119510e+000 1.367447e+001 6.335493e-002
TstI CACNNNNNNTCC 1398 3.323557e+003 3.551349e+007 3.097282e+000 3.259039e+001 5.221745e-002
Bsp68I BtuMI NruI TCGCGA 1410 3.295271e+003 1.232041e+007 3.070922e+000 1.380334e+001 6.453901e-002
BanIII Bsa29I BseCI BshVI BspDI BspXI Bsu15I BsuTUI ClaI ATCGAT 1416 3.281308e+003 1.097055e+007 3.060028e+000 1.111512e+001 6.285311e-002
Alw26I BsmAI BstMAI GTCTC 1427 3.256014e+003 1.186172e+007 3.035739e+000 1.158848e+001 6.447092e-002
AcuI Eco57I CTGAAG 1437 3.233356e+003 1.314021e+007 3.016006e+000 1.206869e+001 7.376479e-002
BtsI GCAGTG 1445 3.215455e+003 1.093510e+007 2.995848e+000 1.077561e+001 6.297578e-002
HgiEII accnnnnnnggt 1473 3.154333e+003 1.004758e+007 2.942974e+000 1.032555e+001 6.720978e-002
Bst6I Eam1104I EarI Ksp632I CTCTTC 1474 3.152193e+003 1.409219e+007 2.934193e+000 1.510700e+001 5.630936e-002
FalI AAGNNNNNCTT 1474 3.152193e+003 3.050588e+007 2.937585e+000 2.757655e+001 4.748982e-002
BsgI GTGCAG 1501 3.095491e+003 1.097475e+007 2.887408e+000 1.120398e+001 6.462358e-002
AceIII cagctc 1541 3.015141e+003 1.147054e+007 2.813108e+000 1.164687e+001 5.515899e-002
Bsp24I gacnnnnnntgg 1574 2.951926e+003 2.502296e+007 2.750318e+000 2.324531e+001 5.336722e-002
HpaI KspAI GTTAAC 1597 2.909413e+003 8.608630e+006 2.715091e+000 9.034691e+000 6.073889e-002
BclI FbaI Ksp22I TGATCA 1608 2.889510e+003 9.116013e+006 2.696517e+000 9.780896e+000 6.094527e-002
AccI FblI XmiI GTMKAC 1628 2.854012e+003 7.904766e+006 2.662776e+000 8.117925e+000 6.081081e-002
UbaF14I ccannnnntcg 1635 2.841793e+003 9.855053e+006 2.645872e+000 1.085187e+001 5.626911e-002
XcmI CCANNNNNNNNNTGG 1636 2.840056e+003 7.702024e+006 2.649144e+000 8.358782e+000 5.440098e-002
AccB7I PflMI Van91I CCANNNNNTGG 1669 2.783902e+003 7.699332e+006 2.596765e+000 8.456608e+000 5.512283e-002
AhaIII DraI tttaaa 1676 2.772274e+003 9.665006e+006 2.584129e+000 9.476500e+000 5.011933e-002
EciI GGCGGA 1677 2.770621e+003 9.730691e+006 2.584973e+000 1.084555e+001 4.949314e-002
Bme1580I BseSI GKGCMC 1690 2.749309e+003 7.832816e+006 2.565089e+000 8.837975e+000 5.562130e-002
AclI Psp1406I AACGTT 1703 2.728322e+003 7.864863e+006 2.544921e+000 8.541900e+000 5.519671e-002
Asp700I MroXI PdmI XmnI GAANNNNTTC 1723 2.696652e+003 7.734721e+006 2.514800e+000 7.966535e+000 5.165409e-002
BstXI CCANNNNNNTGG 1765 2.632483e+003 6.859762e+006 2.456091e+000 7.521455e+000 5.495751e-002
SmlI SmoI CTYRAG 1766 2.630992e+003 7.436425e+006 2.453567e+000 7.494443e+000 5.266138e-002
PvuII CAGCTG 1778 2.613235e+003 7.212090e+006 2.437570e+000 8.039150e+000 5.680540e-002
MlyI PleI PpsI SchI GAGTC 1793 2.591373e+003 7.766945e+006 2.417178e+000 8.520062e+000 5.131065e-002
SimI gggtc 1811 2.565617e+003 8.348525e+006 2.393705e+000 9.197949e+000 5.300939e-002
AgeI AsiGI BshTI CspAI PinAI ACCGGT 1816 2.558553e+003 7.411940e+006 2.386564e+000 8.609715e+000 5.671806e-002
AseI PshBI VspI ATTAAT 1851 2.510174e+003 9.489989e+006 2.341977e+000 9.181907e+000 4.321988e-002
Acc36I BfuAI BspMI BveI ACCTGC 1909 2.433909e+003 6.585783e+006 2.270822e+000 7.519384e+000 4.976427e-002
BglI GCCNNNNNGGC 1919 2.421226e+003 6.760635e+006 2.258989e+000 7.214954e+000 4.846274e-002
BsaAI BstBAI Ppu21I YACGTR 1921 2.418705e+003 6.137467e+006 2.257158e+000 6.363002e+000 5.465903e-002
Bse3DI BseMI BsrDI GCAATG 1965 2.364546e+003 5.940162e+006 2.205598e+000 6.141007e+000 4.936387e-002
BseYI CCCAGC 1975 2.352573e+003 8.338143e+006 2.189873e+000 9.166057e+000 4.506329e-002
BsaBI Bse8I BseJI MamI GATNNNNATC 2013 2.308163e+003 5.508493e+006 2.153502e+000 5.997797e+000 4.520616e-002
Eco32I EcoRV GATATC 2039 2.278731e+003 5.538763e+006 2.126042e+000 6.600887e+000 4.659147e-002
SspI AATATT 2049 2.267610e+003 6.791023e+006 2.115178e+000 7.052157e+000 4.245974e-002
Alw21I Bbv12I BsiHKAI HgiAI GWGCWC 2087 2.226321e+003 5.243449e+006 2.077144e+000 5.667584e+000 4.887398e-002
Acc16I AviII FspI MstI NsbI TGCGCA 2122 2.189600e+003 4.962276e+006 2.041942e+000 5.358448e+000 4.618285e-002
CjeNII gagnnnnngt 2146 2.165113e+003 5.381523e+006 2.019105e+000 5.801500e+000 4.287046e-002
TatI WGTACW 2230 2.083557e+003 4.659027e+006 1.944395e+000 5.157068e+000 4.529148e-002
BsePI BssHII McaTI PauI GCGCGC 2286 2.032516e+003 5.493026e+006 1.895888e+000 6.448675e+000 4.724409e-002
BfmI BpcI BstSFI SfcI SfeI CTRYAG 2298 2.021903e+003 4.920094e+006 1.886423e+000 5.180390e+000 4.438642e-002
Tth111II caarca 2381 1.951420e+003 4.407389e+006 1.819404e+000 4.670733e+000 3.821924e-002
TsoI TARCCA 2425 1.916013e+003 4.046241e+006 1.788041e+000 4.653900e+000 3.835052e-002
UbaF11I tcgta 2453 1.894143e+003 4.093796e+006 1.767631e+000 4.435379e+000 4.565838e-002
BseMII BspCNI CTCAG 2522 1.842320e+003 4.469374e+006 1.719270e+000 4.536788e+000 3.965107e-002
ApaBI BstAPI gcannnnntgc 2555 1.818525e+003 3.381049e+006 1.696282e+000 3.824712e+000 4.070450e-002
AflIII ACRYGT 2588 1.795337e+003 3.131190e+006 1.675039e+000 3.692968e+000 4.018547e-002
BtgZI GCGATG 2716 1.710726e+003 3.804899e+006 1.596097e+000 4.312309e+000 3.865979e-002
BstNSI NspI XceI RCATGY 2783 1.669541e+003 2.831431e+006 1.557672e+000 3.216569e+000 3.521380e-002
Eco57MI CTGRAG 2826 1.644137e+003 3.526315e+006 1.533616e+000 3.566480e+000 4.033970e-002
AvaII Bme18I Eco47I Psp03I SinI VpaK11AI VpaK11BI GGWCC 2882 1.612190e+003 2.967825e+006 1.504511e+000 3.488525e+000 3.504511e-002
BcgI CGANNNNNNTGC 2970 1.564422e+003 7.873454e+006 1.459259e+000 7.919694e+000 2.962963e-002
AcoI CfrI EaeI YGGCCR 3074 1.511494e+003 2.483550e+006 1.410215e+000 2.952723e+000 3.643461e-002
TspGWI ACGGA 3137 1.481139e+003 2.490600e+006 1.381575e+000 2.897403e+000 3.410902e-002
BstX2I BstYI MflI PsuI XhoII RGATCY 3189 1.456987e+003 2.433566e+006 1.359360e+000 2.910970e+000 3.229853e-002
BscGI cccgt 3222 1.442065e+003 2.590532e+006 1.345127e+000 3.017143e+000 3.320919e-002
AlwNI CaiI CAGNNNCTG 3430 1.354616e+003 2.158208e+006 1.263557e+000 2.654344e+000 3.177843e-002
Bsp1286I MhlI SduI GDGCHC 3515 1.321858e+003 1.852295e+006 1.233286e+000 2.379256e+000 3.214794e-002
HaeI wggccw 3720 1.249014e+003 1.688760e+006 1.164785e+000 2.178539e+000 2.956989e-002
AccB1I BanI BshNI BspT107I HgiCI GGYRCC 3727 1.246668e+003 1.760993e+006 1.162866e+000 2.302238e+000 2.924604e-002
BdaI TGANNNNNNTCA 3750 1.239022e+003 4.516743e+006 1.156267e+000 4.597073e+000 2.480000e-002
Bsh1285I BsiEI BstMCI McrI CGRYCG 3846 1.208095e+003 1.902313e+006 1.127145e+000 2.613999e+000 3.120125e-002
AcyI BsaHI BssNI BstACI Hin1I Hsp92I GRCGYC 3916 1.186499e+003 1.661765e+006 1.106742e+000 2.234325e+000 2.630235e-002
BstDSI BtgI DsaI CCRYGG 3992 1.163911e+003 1.368441e+006 1.085671e+000 1.902456e+000 2.905812e-002
AlfI GCANNNNNNTGC 4032 1.152364e+003 4.358883e+006 1.074653e+000 4.617348e+000 2.405754e-002
AclWI AlwI BinI BspPI GGATC 4040 1.150082e+003 1.511377e+006 1.073020e+000 1.959262e+000 2.846535e-002
HincII HindII GTYRAC 4066 1.142728e+003 1.381486e+006 1.066404e+000 1.880952e+000 2.852927e-002
Hin4II HpyAV ccttc 4343 1.069844e+003 1.435723e+006 9.981580e-001 1.851678e+000 2.578863e-002
CjuI caynnnnnrtg 4363 1.064940e+003 1.163592e+006 9.935824e-001 1.640491e+000 2.544121e-002
MslI RseI SmiMI CAYNNNNRTG 4574 1.015814e+003 1.023213e+006 9.477481e-001 1.421061e+000 2.426760e-002
BseGI BstF5I BtsCI FokI StsI GGATG 5240 8.867046e+002 1.011823e+006 8.270992e-001 1.437937e+000 2.080153e-002
TsuI gcgac 5513 8.427956e+002 8.815571e+005 7.865046e-001 1.322517e+000 2.067840e-002
FauI SmuI CCCGC 5580 8.326760e+002 8.940201e+005 7.763441e-001 1.306843e+000 1.881720e-002
AcsI ApoI XapI RAATTY 5725 8.115864e+002 7.501722e+005 7.573799e-001 1.032495e+000 1.956332e-002
CseI HgaI GACGC 5863 7.924837e+002 7.943168e+005 7.392120e-001 1.174046e+000 1.961453e-002
Bse118I BsrFI BssAI Cfr10I RCCGGY 5917 7.852513e+002 7.874513e+005 7.326348e-001 1.297672e+000 2.028055e-002
BetI BsaWI wccggw 5967 7.786714e+002 6.767500e+005 7.263281e-001 1.073097e+000 2.044579e-002
MboII GAAGA 6191 7.504978e+002 8.449782e+005 7.003715e-001 1.117478e+000 1.986755e-002
BceAI BcefI ACGGC 6282 7.396262e+002 6.490299e+005 6.902260e-001 1.022955e+000 1.798790e-002
TspDTI ATGAA 6391 7.270117e+002 7.461011e+005 6.784541e-001 9.530866e-001 1.815052e-002
BccI CCATC 6485 7.164737e+002 6.041178e+005 6.686199e-001 9.270303e-001 1.757903e-002
BstH2I HaeII LpnI RGCGCY 6542 7.102311e+002 6.090081e+005 6.624885e-001 9.696950e-001 1.880159e-002
BstDEI DdeI HpyF3I CTNAG 6816 6.816802e+002 5.491098e+005 6.361502e-001 7.758845e-001 1.760563e-002
HaeIV gaynnnnnrtc 6930 6.704664e+002 1.324798e+006 6.248196e-001 1.486583e+000 1.558442e-002
BscAI LweI SfaNI gcatc 7036 6.603655e+002 4.956154e+005 6.162592e-001 8.167591e-001 1.563388e-002
AspS9I AsuI BmgT120I Cfr13I FmuI Sau96I UnbI GGNCC 7050 6.590542e+002 4.996169e+005 6.150355e-001 8.280900e-001 1.673759e-002
PfeI TfiI GAWTC 7176 6.474822e+002 4.303366e+005 6.042363e-001 7.077395e-001 1.672241e-002
CdiI catcg 7201 6.452343e+002 4.841370e+005 6.021386e-001 7.979343e-001 1.597000e-002
Bse1I BseNI BsrI BsrSI ACTGG 7318 6.349183e+002 6.216771e+005 5.922383e-001 8.868716e-001 1.598797e-002
AsuHPI HphI SspD5I GGTGA 7406 6.273740e+002 4.696033e+005 5.854712e-001 7.612960e-001 1.674318e-002
BspNCI ccaga 7513 6.184390e+002 5.182914e+005 5.769999e-001 8.034752e-001 1.570611e-002
NmuCI Tsp45I GTSAC 7787 5.966781e+002 3.712461e+005 5.568255e-001 6.513749e-001 1.630923e-002
CjePI ccannnnnnntc 8034 5.783336e+002 1.083957e+006 5.390839e-001 1.255599e+000 1.319393e-002
MnlI CCTC 8050 5.771841e+002 4.180919e+005 5.386335e-001 6.309461e-001 1.391304e-002
MspA1I NspBII CMGCKG 8246 5.634650e+002 3.668327e+005 5.255882e-001 6.627169e-001 1.540141e-002
BmiI BspLI NlaIV PspN4I GGNNCC 8430 5.511663e+002 3.561167e+005 5.143535e-001 6.652940e-001 1.506524e-002
BbvI BseXI BstV1I GCAGC 8860 5.244167e+002 3.423479e+005 4.892777e-001 6.025490e-001 1.455982e-002
AsuC2I BcnI BpuMI CauII EcoHI NciI CCSGG 9003 5.160871e+002 3.113374e+005 4.816172e-001 5.973903e-001 1.366211e-002
CjeI ccannnnnngt 9120 5.094662e+002 8.303013e+005 4.752193e-001 1.026472e+000 1.195175e-002
Hin4I GAYNNNNNVTC 9162 5.071308e+002 7.442203e+005 4.729317e-001 9.083929e-001 1.244270e-002
AspCNI gccgc 9407 4.939228e+002 3.506401e+005 4.607207e-001 6.409990e-001 1.254385e-002
TspRI CASTG 10378 4.477098e+002 2.162163e+005 4.178069e-001 4.377363e-001 1.194835e-002
Hpy99I CGWCG 10379 4.476666e+002 2.671630e+005 4.176703e-001 5.348236e-001 1.204355e-002
HinfI GANTC 10764 4.316548e+002 1.914271e+005 4.028242e-001 4.018725e-001 1.189149e-002
BsaJI BseDI BssECI SecI CCNNGG 11546 4.024192e+002 1.722814e+005 3.754547e-001 4.008145e-001 1.143253e-002
AfaI Csp6I CviQI PabI RsaI GTAC 12048 3.856517e+002 1.659786e+005 3.598938e-001 3.919234e-001 1.103918e-002
AjnI BptI BseBI BstNI BstOI Bst2UI EcoRII MvaI Psp6I PspGI CCWGG 12050 3.855877e+002 1.501643e+005 3.597510e-001 3.667922e-001 1.095436e-002
Hpy188I TCNGA 12183 3.813783e+002 1.633600e+005 3.558237e-001 3.627076e-001 1.099893e-002
BshFI BsnI BspANI BsuRI HaeIII PhoI GGCC 12631 3.678515e+002 1.537638e+005 3.432824e-001 3.625913e-001 1.045048e-002
Hpy8I MjaIV GTNNAC 12999 3.574376e+002 1.284045e+005 3.335641e-001 3.315638e-001 1.007770e-002
AluI AGCT 13349 3.480659e+002 1.262593e+005 3.248183e-001 3.155220e-001 9.888381e-003
AfiI Bsc4I BseLI BsiYI BslI CCNNNNNNNGG 14283 3.253050e+002 1.138314e+005 3.035777e-001 3.202414e-001 8.961703e-003
HpyCH4IV MaeII TaiI ACGT 14552 3.192916e+002 1.107344e+005 2.979659e-001 2.997746e-001 9.070918e-003
CviAII FaeI FatI Hin1II Hsp92II NlaIII CATG 15347 3.027518e+002 9.859123e+004 2.824656e-001 2.674646e-001 8.210074e-003
EsaBC3I TaqI tcga 15482 3.001119e+002 1.032079e+005 2.800672e-001 2.826451e-001 8.526030e-003
MaeIII GTNAC 16065 2.892208e+002 8.772337e+004 2.699035e-001 2.616844e-001 8.216620e-003
Hpy178III Hpy188III tcnnga 16091 2.887535e+002 8.860696e+004 2.694674e-001 2.577744e-001 8.203343e-003
Sth132I cccg 16382 2.836242e+002 9.812628e+004 2.646807e-001 2.720435e-001 8.057624e-003
ApeKI TseI GCWGC 17203 2.700885e+002 8.669780e+004 2.519909e-001 2.553552e-001 7.847468e-003
Bst4CI HpyCH4III TaaI Tsp4CI ACNGT 17420 2.667240e+002 7.576156e+004 2.489093e-001 2.433394e-001 7.749713e-003
TauI GCSGC 18657 2.490396e+002 8.737231e+004 2.323525e-001 2.623152e-001 6.807097e-003
BfuCI Bsp143I BssMI BstKTI BstMBI ChaI DpnI DpnII Kzo9I MalI MboI NdeII Sau3AI GATC 19146 2.426790e+002 7.186618e+004 2.264703e-001 2.281549e-001 6.894390e-003
Sse9I TasI Tsp509I TspEI AATT 19652 2.364305e+002 7.811461e+004 2.206391e-001 2.078932e-001 6.513332e-003
CviRI HpyCH4V tgca 19774 2.349718e+002 5.707172e+004 2.192778e-001 2.044814e-001 6.726004e-003
Bme1390I BmrFI BssKI BstSCI MspR9I ScrFI StyD4I CCNGG 21053 2.206969e+002 5.073408e+004 2.059564e-001 1.967021e-001 6.459887e-003
MseI Tru1I Tru9I TTAA 21194 2.192286e+002 6.332636e+004 2.045862e-001 1.890678e-001 6.369727e-003
BsiSI HapII HpaII MspI Sth302II CCGG 24357 1.907596e+002 4.594984e+004 1.780186e-001 1.805761e-001 5.501499e-003
AccII Bsh1236I BstFNI BstUI FnuDII MvnI SelI CGCG 26267 1.768886e+002 4.605966e+004 1.650740e-001 1.738460e-001 5.177599e-003
AciI BspACI SsiI CCGC 27622 1.682113e+002 3.830414e+004 1.569763e-001 1.578274e-001 4.815003e-003
BstC8I Cac8I GCNNGC 27697 1.677558e+002 3.644334e+004 1.565513e-001 1.555168e-001 4.910279e-003
BstMWI HpyF10VI MwoI GCNNNNNNNGC 30731 1.511936e+002 2.784717e+004 1.410953e-001 1.379828e-001 4.458039e-003
AspLEI BstHHI CfoI GlaI HhaI Hin6I HinP1I HspAI GCGC 32830 1.415270e+002 2.844485e+004 1.320439e-001 1.324618e-001 4.142553e-003
BisI BlsI BthCI Fnu4HI Fsp4HI GluI ItaI SatI GCNGC 34103 1.362441e+002 2.407428e+004 1.271149e-001 1.254459e-001 4.017242e-003
SetI ASST 54885 8.465577e+001 6.841718e+003 7.900155e-002 7.447433e-002 2.477908e-003
CviJI CviKI-1 RGCY 61298 7.579908e+001 5.705150e+003 7.073640e-002 6.703896e-002 2.234983e-003