Team:ETH Zurich/Modeling/Switch Circuit

From 2008.igem.org

(Difference between revisions)
(Detailed Model)
(Detailed Model)
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==== diffusion of IPTG ====
==== diffusion of IPTG ====
 +
[[Image:diffusion_IPTG.jpg|left|300px|]]
==== diffusion of tet ====
==== diffusion of tet ====
 +
[[Image:diffusiom_tet.jpg|left|300px|]]
==== binding of tetR to LacI-promotor and LacIIs-promotor ====
==== binding of tetR to LacI-promotor and LacIIs-promotor ====
 +
[[Image:binding_tetR_to_LacI.jpg|left|300px|]]
==== binding of LacI and LacIIs to GFP-promotor ====
==== binding of LacI and LacIIs to GFP-promotor ====
 +
[[Image:binding_LacIIs_to_Pgfp.jpg|left|300px|]]
 +
[[Image:binding_LacI_to_Pgfp.jpg|left|300px|]]
==== binding of tet to tetR ====
==== binding of tet to tetR ====
 +
[[Image:binding_tet_to_tetR.jpg|left|300px|]]
==== binding of IPTG to LacI ====
==== binding of IPTG to LacI ====
 +
[[Image:binding_IPTG_to_LacI.jpg|left|300px|]]
==== transcription and translation of LacI ====
==== transcription and translation of LacI ====
 +
[[Image:transcription_of_LacI.jpg|left|300px|]]
 +
[[Image:translation_of_LacI.jpg|left|300px|]]
==== transcription and translation of LacIIs ====
==== transcription and translation of LacIIs ====
 +
[[Image:transcription_of_LacIIs.jpg|left|300px|]]
 +
[[Image:translation_of_LacIIs.jpg|left|300px|]]
==== transcription and translation of tetR ====
==== transcription and translation of tetR ====
 +
[[Image:transcription_of_tetR.jpg|left|300px|]]
 +
[[Image:translation_of_tetR.jpg|left|300px|]]
==== transcription and translation of GFP ====
==== transcription and translation of GFP ====
 +
[[Image:transcription_of_gfp.jpg|left|300px|]]
 +
[[Image:translation_of_gfp.jpg|left|300px|]]
==== dimerization of tetR ====
==== dimerization of tetR ====
 +
[[Image:dimerization_of_tetR.jpg|left|300px|]]
==== dimerization and tetramerization of LacI and LacIIs ====
==== dimerization and tetramerization of LacI and LacIIs ====
 +
[[Image:dimerization_of_LacI.jpg|left|300px|]]
 +
[[Image:dimerization_of_LacIIs.jpg|left|300px|]]
 +
[[Image:tetramerization_of_LacI.jpg|left|300px|]]
 +
[[Image:tetramerization_of_LacIIs.jpg|left|300px|]]
=== Implementation ===
=== Implementation ===

Revision as of 12:57, 27 October 2008

Contents

Switch Circuit

Detailed Model

diffusion of IPTG

diffusion of tet

binding of tetR to LacI-promotor and LacIIs-promotor

binding of LacI and LacIIs to GFP-promotor

binding of tet to tetR

binding of IPTG to LacI

transcription and translation of LacI

transcription and translation of LacIIs

transcription and translation of tetR

transcription and translation of GFP

dimerization of tetR

dimerization and tetramerization of LacI and LacIIs

Implementation

Simulation

Results and Discussion

Parameters

References