Team:Edinbrugh/Results/Glycogen 4

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Glycogen Assay 4 (Quantitative: Raman)

Raman spectroscopy was performed by Dr Rabah Mouras.

Experiment Design

See Glycogen Assay 3 for explanation.

Legend

Organisms:

  • Sample 1: Control strain of E. coli containing a plasmid that should have no effect on glycogen levels.
  • Sample 2: E. coli overexpressing glgC.
  • Sample 3: E. coli overexpressing glgC16.

Growth Conditions:

  • Condition A: normal growth medium (LB, or L-broth).
  • Condition B: LB + 10 mM glucose.
  • Condition C: LB + 25 mM glucose.
  • Condition D: LB + 50 mM glucose.

It is expected that the presence of higher levels of glucose, up to a point, will increase the amount of glycogen produced by the cells.

Results

Edinburgh=Glycogenraman1.jpg Edinburgh=Glycogenraman2.jpg Edinburgh=Glycogenraman3.jpg

Analysis forthcoming.