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Team:Edinburgh/Protocols
From 2008.igem.org
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* [[Team:Edinburgh/Protocols/Edinbrick1|Use of Edinbrick1 in preparation of BioBricks<sup>TM</sup> from PCR products]] | * [[Team:Edinburgh/Protocols/Edinbrick1|Use of Edinbrick1 in preparation of BioBricks<sup>TM</sup> from PCR products]] | ||
| - | * [[Team:Edinburgh/ | + | * [[Team:Edinburgh/Protocols/MABEL|Site-directed mutagenesis using the MABEL protocol]] |
* [[Team:Edinburgh/Results/BABEL|BABEL: BioBrick<sup>TM</sup> assembly with blunt-ended ligation]] | * [[Team:Edinburgh/Results/BABEL|BABEL: BioBrick<sup>TM</sup> assembly with blunt-ended ligation]] | ||
* [[Team:Edinburgh/Results/Bacillobricks|Bacillobricks: introduction of BioBricks into ''Bacillus subtilis'']] | * [[Team:Edinburgh/Results/Bacillobricks|Bacillobricks: introduction of BioBricks into ''Bacillus subtilis'']] | ||
Revision as of 02:12, 30 October 2008
Protocols
As part of our project we developed and tested a number of procedures to make BioBrick production easier, more reliable and less hassle. These are the following:
- Use of Edinbrick1 in preparation of BioBricksTM from PCR products
- Site-directed mutagenesis using the MABEL protocol
- BABEL: BioBrickTM assembly with blunt-ended ligation
- Bacillobricks: introduction of BioBricks into Bacillus subtilis
