Team:Edinburgh/SU1

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SU1

Amino Acid Sequence

MAQQLPCVSSPRPlLAvPAGRWRAGvRGRPNvAGlGRGRlSLHAAAARPv
AEAvQAEEDDDDDDEEvAEERFAlGGACRvLAGMPAPLGATALRGGVNFA
VYSSGASAASlCLFAPGDLKADRvTEEvPLDPlLNRTGNvWHvFIHGDQl
HGMLYGYRFDGvFAPERGQYYDvSNVvvDPYAKAvVSRGEYGvPAPGGSC
WPQMAGMIPLPYNKFDWQGDLPLGYHQKDLVIYEMHlRGFTKHNSSKTKH
PGTYIGAvSKLDHLKELGvNCIELMPCHEFNELEYFSSSSKMNFWGYSTI
NFFSPMARYSSSGIRDSGCGAINEFKAFVREAHKRGIEvIMDVVFNHTAE
GNEKGPILSFRGIDNSTYYMLAPKGEFYNYSGCGNTFNCNHPVVREFIvD
ClRYWVTEMHVDGFRFDLASIlTRGCSLWDPVNvYGSPMEGDMITTGTPL
VAPPLIDMISNDPILGNVKLIAEAWDAGGLYQVGQFPHWNVWSEWNGKYR
DTvRQFIKGTDGFAGAFAECLCGSPQLYQAGGRKPWHSINFVCAHDGFTl
ADlVTYNSKYNlSNGEDNRDGENHNLSWNCGEEGEFASlSVRRLRKRQMR
NFFVCLMVSQGVPMFYMGDEYGHTKGGNNNTYCHDHYVNYFRWDKKEEQS
SDlYRFCRLMTKFRKECESLGLEDFPTSERlKWHGHQPGKPDWSEASRFV
AFTMKDETKGEIYvAFNTSHLPvVVGLPERSGFRWEPvvDTGKEAPYDFL
TDGLPDRAVTVYQFSHFLNSNLYPMLSYSSIILVlRPDV*

DNA Sequence

atggcgcagcagctcccctgcgtctcgtcgccgcgcccgctgctcgccgt 50
accgcgtcgtcgaggggacgcagagcagcggcgcgggcgacgagcggca

gcccgcgggccggtggcgcgccggcgtgcggggccggcccaatgtggcgg 100
cgggcgcccggccaccgcgcggccgcacgccccggccgggttacaccgcc

gactggggcgggggcggctgtctctccacgccgccgccgcgcggcccgtg 150
ctgaccccgcccccgccgacagagaggtgcggcggcggcgcgccgggcac

gccgaggcggtgcaggcggaggaggacgacgacgacgacgacgaggaggt 200
cggctccgccacgtccgcctcctcctgctgctgctgctgctgctcctcca

ggccgaggagaggttcgcgctgggcggcgcgtgccgggtgctcgcgggaa 250
ccggctcctctccaagcgcgacccgccgcgcacggcccacgagcgccctt

tgcccgcgccgctcggcgccaccgcgctccgcggcggtgtcaacttcgcc 300
acgggcgcggcgagccgcggtggcgcgaggcgccgccacagttgaagcgg

gtctactccagcggtgcctccgccgcgtcgctgtgcctcttcgctcccgg 350
cagatgaggtcgccacggaggcggcgcagcgacacggagaagcgagggcc

cgacctcaaggcggatagggtgaccgaggaggtgcccctcgatcccctgc 400
gctggagttccgcctatcccactggctcctccacggggagctaggggacg

tcaaccgaacgggaaacgtgtggcacgtgttcatccacggggaccagctg 450
agttggcttgccctttgcacaccgtgcacaagtaggtgcccctggtcgac

cacggcatgctctacggatacaggttcgatggcgtgttcgcccctgagcg 500
gtgccgtacgagatgcctatgtccaagctaccgcacaagcggggactcgc

cggacagtactacgatgtgtccaacgttgtggtggatccatacgctaagg 550
gcctgtcatgatgctacacaggttgcaacaccacctaggtatgcgattcc

cagtggtaagccgaggtgaatatggtgtgcctgcgcctggtggtagttgt 600
gtcaccattcggctccacttataccacacggacgcggaccaccatcaaca

tggcctcaaatggctggtatgatccctcttccctataataagtttgattg 650
accggagtttaccgaccatactagggagaagggatattattcaaactaac

gcaaggtgacctaccccttgggtaccatcagaaggaccttgtcatatatg 700
cgttccactggatggggaacccatggtagtcttcctggaacagtatatac

aaatgcatttgcgtggattcacaaagcacaactcaagcaagacaaaacac 750
tttacgtaaacgcacctaagtgtttcgtgttgagttcgttctgttttgtg

ccaggaacttacattggtgctgtgtcaaagcttgaccatctaaaggaact 800
ggtccttgaatgtaaccacgacacagtttcgaactggtagatttccttga

tggagtgaactgtatagagctaatgccctgccatgagttcaatgagctag 850
acctcacttgacatatctcgattacgggacggtactcaagttactcgatc

agtacttcagctcctcttcgaagatgaacttctggggatattccacaata 900
tcatgaagtcgaggagaagcttctacttgaagacccctataaggtgttat

aattttttctcaccaatggcaagatattcttcaagtggcataagagactc 950
ttaaaaaagagtggttaccgttctataagaagttcaccgtattctctgag

tggatgtggtgccataaatgaatttaaagcttttgtaagggaggcccaca 1000
acctacaccacggtatttacttaaatttcgaaaacattccctccgggtgt

aacggggaattgaggtgatcatggatgttgtcttcaatcatacagctgaa 1050
ttgccccttaactccactagtacctacaacagaagttagtatgtcgactt

ggtaatgagaaaggcccaatattatcctttagggggatagataatagtac 1100
ccattactctttccgggttataataggaaatccccctatctattatcatg

atactacatgcttgcacctaagggagagttttataattattctggttgtg 1150
tatgatgtacgaacgtggattccctctcaaaatattaataagaccaacac

gaaataccttcaattgtaatcatcctgtagtccgtgaatttatagtggat 1200
ctttatggaagttaacattagtaggacatcaggcacttaaatatcaccta

tgcttgagatactgggtaacagaaatgcatgttgatggttttcgttttga 1250
acgaactctatgacccattgtctttacgtacaactaccaaaagcaaaact

ccttgcatctatactgaccagaggatgcagtctatgggatccagttaatg 1300
ggaacgtagatatgactggtctcctacgtcagataccctaggtcaattac

tgtatggaagtccaatggaaggtgacatgattacgacagggacacctctt 1350
acataccttcaggttaccttccactgtactaatgctgtccctgtggagaa

gttgccccaccacttattgacatgattagcaatgacccaattcttggaaa 1400
caacggggtggtgaataactgtactaatcgttactgggttaagaaccttt

tgtcaagctcattgctgaagcatgggatgcaggaggtctctatcaagttg 1450
acagttcgagtaacgacttcgtaccctacgtcctccagagatagttcaac

gtcagtttcctcactggaacgtttggtcagagtggaatggaaagtatcgc 1500
cagtcaaaggagtgaccttgcaaaccagtctcaccttacctttcatagcg

gataccgtgcgtcagttcatcaaaggcacagatggatttgctggtgcttt 1550
ctatggcacgcagtcaagtagtttccgtgtctacctaaacgaccacgaaa

tgctgaatgcctatgtggaagtccacagttataccaggcaggggggagga 1600
acgacttacggatacaccttcaggtgtcaatatggtccgtcccccctcct

agccttggcacagtatcaactttgtatgtgcacacgatggatttacactg 1650
tcggaaccgtgtcatagttgaaacatacacgtgtgctacctaaatgtgac

gctgatttggtcacatacaatagcaagtacaacttgtcaaatggtgagga 1700
cgactaaaccagtgtatgttatcgttcatgttgaacagtttaccactcct

caacagagatggggaaaatcataatcttagctggaattgtggggaggaag 1750
gttgtctctaccccttttagtattagaatcgaccttaacacccctccttc

gagaatttgcaagtctgtcagtccgaagattaaggaagaggcaaatgcgc 1800
ctcttaaacgttcagacagtcaggcttctaattccttctccgtttacgcg

aatttctttgtttgtcttatggtttctcagggagttccaatgttctacat 1850
ttaaagaaacaaacagaataccaaagagtccctcaaggttacaagatgta

gggcgatgaatatggtcacacaaagggagggaacaacaatacgtactgcc 1900
cccgctacttataccagtgtgtttccctcccttgttgttatgcatgacgg

atgaccattatgtcaactatttccgttgggataagaaggaagaacaatcc 1950
tactggtaatacagttgataaaggcaaccctattcttccttcttgttagg

tctgatttgtacagattctgccgtctcatgaccaaattccgcaaggaatg 2000
agactaaacatgtctaagacggcagagtactggtttaaggcgttccttac

tgaatctcttggccttgaggacttcccgacttcagaacggttgaaatggc 2050
acttagagaaccggaactcctgaagggctgaagtcttgccaactttaccg

acggtcatcagcccgggaagcctgactggtcagaggcaagccgattcgtt 2100
tgccagtagtcgggcccttcggactgaccagtctccgttcggctaagcaa

gccttcaccatgaaggacgaaaccaaaggcgagatctacgtggccttcaa 2150
cggaagtggtacttcctgctttggtttccgctctagatgcaccggaagtt

caccagtcaccttccggtggttgtcgggcttccagagcgctctgggttcc 2200
gtggtcagtggaaggccaccaacagcccgaaggtctcgcgagacccaagg

gatgggagccggtggtggacaccggcaaggaggcaccatatgacttcctc 2250
ctaccctcggccaccacctgtggccgttcctccgtggtatactgaaggag

accgatggcctaccagatcgtgctgtcaccgtctaccagttctctcattt 2300
tggctaccggatggtctagcacgacagtggcagatggtcaagagagtaaa

cctcaactccaatctctatcctatgctcagctactcctccatcatccttg 2350
ggagttgaggttagagataggatacgagtcgatgaggaggtagtaggaac

tattgcgccctgatgtctga 2370
ataacgcgggactacagact

No forbidden restriction sites.

Primers

Forward: gat gaattc gcggccgc t tctag atg gcg cag ctc ccc tg
Reverse: gct actagt a tta tta gac atc agg gcg caa tac