Team:Edinburgh/crtB

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Contents

crtB

AMINO ACID SEQUENCE

MNNPSLLNHAVETMAVGSKSFATASKLFDAKTRRSVLMLYAWCR
HCDDVIDDQTLGFQARQPALQTPEQRLMQLEMKTRQAYAGSQMHEPAFAAFQEVAMAH
DIAPAYAFDHLEGFAMDVREAQYSQLDDTLRYCYHVAGVVGLMMAQIMGVRDNATLDR
ACDLGLAFQLTNIARDIVDDAHAGRCYLPASWLEHEGLNKENYAAPENRQALSRIARR
LVQEAEPYYLSATAGLAGLPLRSAWAIATAKQVYRKIGVKVEQAGQQAWDQRQSTTTP
EKLTLLLAASGQALTSRMRAHPPRPAHLWQRPL*

DNA SEQUENCE

atgaataatccgtcgttactcaatcatgcggtcgaaacgatggc
agttggctcgaaaagttttgcgacagcctcaaagttatttgatgcaaaaacccggcgcag
cgtactgatgctctacgcctggtgccgccattgtgacgatgttattgacgatcagacgct
gggctttcaggcccggcagcctgccttacaaacgcccgaacaacgtctgatgcaacttga
gatgaaaacgcgccaggcctatgcaggatcgcagatgcacgaaccggcgtttgcggcttt
tcaggaagtggctatggctcatgatatcgccccggcttacgcgtttgatcatctggaagg
cttcgccatggatgtacgcgaagcgcaatacagccaactggatgatacgctgcgctattg
ctatcacgttgcaggcgttgtcggcttgatgatggcgcaaatcatgggcgtgcgggataa
cgccacgctggaccgcgcctgtgaccttgggctggcatttcagttgaccaatattgctcg
cgatattgtggacgatgcgcatgcgggccgctgttatctgccggcaagctggctggagca
tgaaggtctgaacaaagagaattatgcggcacctgaaaaccgtcaggcgctgagccgtat
cgcccgtcgtttggtgcaggaagcagaaccttactatttgtctgccacagccggcctggc
agggttgcccctgcgttccgcctgggcaatcgctacggcgaagcaggtttaccggaaaat
aggtgtcaaagttgaacaggccggtcagcaagcctgggatcagcggcagtcaacgaccac
gcccgaaaaattaacgctgctgctggccgcctctggtcaggcccttacttcccggatgcg
ggctcatcctccccgccctgcgcatctctggcagcgcccgctctaataa

No forbidden restriction sites

BIOBRICKTM PRIMERS

Forward: gat gaattc gcggccgc t tctag atg aat aat ccg tcg tta ctc
Reverse: cgt actagt a tta tta gag cgg gcg ctg cca g (reverse primer from Edinburgh iGEM 2007)