Team:Guelph/Notebook

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Notebook

To Do List

-redo PCR of CrtI with giant primers to eliminate PstI sites and cut the PCR product with PstI to select only for "fixed" fragments (Dave and Lisa)

-cut and ligate the synthetic operon now that we have the genes cloned (Lisa, Ed, Brendan)

-test GFP tagged Klebsiella (Tin) and E. coli nissler (Brendan) in vivo to ensure plasmids work and those microbes are competitive

-attempt another lactobacilli electroporation (Brendan and Tin)

-cut and ligate RNAi constructs together (Moffman and Jen)

-work out deal to print T-shirts (Moffman)

-write more grants to Special grants and CSA - other fundraising ideas welcome (Jen)

Calendar

May
M	T	W	T	F	S	S
			[http://2008.igem.org/Guelph/1_May_2008 1]	[http://2008.igem.org/Guelph/2_May_2008 2]	[http://2008.igem.org/wiki/index.php?title=Guelph/3_May_2008&action=edit 3]	[http://2008.igem.org/wiki/index.php?title=Guelph/4_May_2008&action=edit 4]
[http://2008.igem.org/wiki/index.php?title=Guelph/5_May_2008&action=edit 5]	[http://2008.igem.org/wiki/index.php?title=Guelph/6_May_2008&action=edit 6]	[http://2008.igem.org/wiki/index.php?title=Guelph/7_May_2008&action=edit 7]	[http://2008.igem.org/wiki/index.php?title=Guelph/8_May_2008&action=edit 8]	[http://2008.igem.org/wiki/index.php?title=Guelph/9_May_2008&action=edit 9]	[http://2008.igem.org/wiki/index.php?title=Guelph/10_May_2008&action=edit 10]	[http://2008.igem.org/wiki/index.php?title=Guelph/11_May_2008&action=edit 11]
[http://2008.igem.org/wiki/index.php?title=Guelph/12_May_2008&action=edit 12]	[http://2008.igem.org/wiki/index.php?title=Guelph/13_May_2008&action=edit 13]	[http://2008.igem.org/wiki/index.php?title=Guelph/14_May_2008&action=edit 14]	[http://2008.igem.org/wiki/index.php?title=Guelph/15_May_2008&action=edit 15]	[http://2008.igem.org/wiki/index.php?title=Guelph/16_May_2008&action=edit 16]	[http://2008.igem.org/wiki/index.php?title=Guelph/17_May_2008&action=edit 17]	[http://2008.igem.org/wiki/index.php?title=Guelph/18_May_2008&action=edit 18]
[http://2008.igem.org/wiki/index.php?title=Guelph/19_May_2008&action=edit 19]	[http://2008.igem.org/wiki/index.php?title=Guelph/20_May_2008&action=edit 20]	[http://2008.igem.org/wiki/index.php?title=Guelph/21_May_2008&action=edit 21]	[http://2008.igem.org/wiki/index.php?title=Guelph/22_May_2008&action=edit 22]	[http://2008.igem.org/wiki/index.php?title=Guelph/23_May_2008&action=edit 23]	[http://2008.igem.org/wiki/index.php?title=Guelph/24_May_2008&action=edit 24]	[http://2008.igem.org/wiki/index.php?title=Guelph/25_May_2008&action=edit 25]
[http://2008.igem.org/wiki/index.php?title=Guelph/26_May_2008&action=edit 26]	[http://2008.igem.org/wiki/index.php?title=Guelph/27_May_2008&action=edit 27]	[http://2008.igem.org/wiki/index.php?title=Guelph/28_May_2008&action=edit 28]	[http://2008.igem.org/Guelph/29_May_2008 29]	[http://2008.igem.org/wiki/index.php?title=Guelph/30_May_2008&action=edit 30]	[http://2008.igem.org/wiki/index.php?title=Guelph/31_May_2008&action=edit 31]

June
M	T	W	T	F	S	S
						[http://2008.igem.org/Guelph/1_June_2008 1]
[http://2008.igem.org/wiki/index.php?title=Guelph/2_June_2008&action=edit 2]	[http://2008.igem.org/Guelph/3_June_2008 3]	[http://2008.igem.org/wiki/index.php?title=Guelph/4_June_2008&action=edit 4]	[http://2008.igem.org/Guelph/5_June_2008 5]	[http://2008.igem.org/wiki/index.php?title=Guelph/6_June_2008&action=edit 6]	[http://2008.igem.org/wiki/index.php?title=Guelph/7_June_2008&action=edit 7]	[http://2008.igem.org/wiki/index.php?title=Guelph/8_June_2008&action=edit 8]
[http://2008.igem.org/wiki/index.php?title=Guelph/9_June_2008&action=edit 9]	[http://2008.igem.org/Guelph/10_June_2008 10]	[http://2008.igem.org/wiki/index.php?title=Guelph/11_June_2008&action=edit 11]	[http://2008.igem.org/wiki/index.php?title=Guelph/12_June_2008&action=edit 12]	[http://2008.igem.org/wiki/index.php?title=Guelph/13_June_2008&action=edit 13]	[http://2008.igem.org/wiki/index.php?title=Guelph/14_June_2008&action=edit 14]	[http://2008.igem.org/wiki/index.php?title=Guelph/15_June_2008&action=edit 15]
[http://2008.igem.org/Guelph/16_June_2008 16]	[http://2008.igem.org/wiki/index.php?title=Guelph/17_June_2008&action=edit 17]	[http://2008.igem.org/wiki/index.php?title=Guelph/18_June_2008&action=edit 18]	[http://2008.igem.org/Guelph/19_June_2008 19]	[http://2008.igem.org/Guelph/20_June_2008 20]	[http://2008.igem.org/wiki/index.php?title=Guelph/21_June_2008&action=edit 21]	[http://2008.igem.org/wiki/index.php?title=Guelph/22_June_2008&action=edit 22]
[http://2008.igem.org/Guelph/23_June_2008 23]	[http://2008.igem.org/wiki/index.php?title=Guelph/24_June_2008&action=edit 24]	[http://2008.igem.org/wiki/index.php?title=Guelph/25_June_2008&action=edit 25]	[http://2008.igem.org/Guelph/26_June_2008 26]	[http://2008.igem.org/wiki/index.php?title=Guelph/27_June_2008&action=edit 27]	[http://2008.igem.org/wiki/index.php?title=Guelph/28_June_2008&action=edit 28]	[http://2008.igem.org/Guelph/29_June_2008 29]
[http://2008.igem.org/wiki/index.php?title=Guelph/30_June_2008&action=edit 30]

July
M	T	W	T	F	S	S
	[http://2008.igem.org/wiki/index.php?title=Guelph/1_July_2008&action=edit 1]	[http://2008.igem.org/Guelph/2_July_2008 2]	[http://2008.igem.org/Guelph/3_July_2008 3]	[http://2008.igem.org/wiki/index.php?title=Guelph/4_July_2008&action=edit 4]	[http://2008.igem.org/wiki/index.php?title=Guelph/5_July_2008&action=edit 5]	[http://2008.igem.org/wiki/index.php?title=Guelph/6_July_2008&action=edit 6]
[http://2008.igem.org/Guelph/7_July_2008 7]	[http://2008.igem.org/Guelph/8_July_2008 8]	[http://2008.igem.org/wiki/index.php?title=Guelph/9_July_2008&action=edit 9]	[http://2008.igem.org/wiki/index.php?title=Guelph/10_July_2008&action=edit 10]	[http://2008.igem.org/wiki/index.php?title=Guelph/11_July_2008&action=edit 11]	[http://2008.igem.org/wiki/index.php?title=Guelph/12_July_2008&action=edit 12]	[http://2008.igem.org/wiki/index.php?title=Guelph/13_July_2008&action=edit 13]
[http://2008.igem.org/Guelph/14_July_2008 14]	[http://2008.igem.org/wiki/index.php?title=Guelph/15_July_2008&action=edit 15]	[http://2008.igem.org/wiki/index.php?title=Guelph/16_July_2008&action=edit 16]	[http://2008.igem.org/wiki/index.php?title=Guelph/17_July_2008&action=edit 17]	[http://2008.igem.org/Guelph/18_July_2008 18]	[http://2008.igem.org/wiki/index.php?title=Guelph/19_July_2008&action=edit 19]	[http://2008.igem.org/wiki/index.php?title=Guelph/20_July_2008&action=edit 20]
[http://2008.igem.org/Guelph/21_July_2008 21]	[http://2008.igem.org/wiki/index.php?title=Guelph/22_July_2008&action=edit 22]	[http://2008.igem.org/wiki/index.php?title=Guelph/23_July_2008&action=edit 23]	[http://2008.igem.org/Guelph/24_July_2008 24]	[http://2008.igem.org/wiki/index.php?title=Guelph/25_July_2008&action=edit 25]	[http://2008.igem.org/wiki/index.php?title=Guelph/26_July_2008&action=edit 26]	[http://2008.igem.org/wiki/index.php?title=Guelph/27_July_2008&action=edit 27]
[http://2008.igem.org/wiki/index.php?title=Guelph/28_July_2008&action=edit 28]	[http://2008.igem.org/wiki/index.php?title=Guelph/29_July_2008&action=edit 29]	[http://2008.igem.org/wiki/index.php?title=Guelph/30_July_2008&action=edit 30]	[http://2008.igem.org/wiki/index.php?title=Guelph/31_July_2008&action=edit 31]

August
M	T	W	T	F	S	S
				[http://2008.igem.org/Guelph/1_August_2008 1]	[http://2008.igem.org/wiki/index.php?title=Guelph/2_August_2008&action=edit 2]	[http://2008.igem.org/wiki/index.php?title=Guelph/3_August_2008&action=edit 3]
[http://2008.igem.org/wiki/index.php?title=Guelph/4_August_2008&action=edit 4]	[http://2008.igem.org/wiki/index.php?title=Guelph/5_August_2008&action=edit 5]	[http://2008.igem.org/Guelph/6_August_2008 6]	[http://2008.igem.org/wiki/index.php?title=Guelph/7_August_2008&action=edit 7]	[http://2008.igem.org/wiki/index.php?title=Guelph/8_August_2008&action=edit 8]	[http://2008.igem.org/wiki/index.php?title=Guelph/9_August_2008&action=edit 9]	[http://2008.igem.org/wiki/index.php?title=Guelph/10_August_2008&action=edit 10]
[http://2008.igem.org/wiki/index.php?title=Guelph/11_August_2008&action=edit 11]	[http://2008.igem.org/wiki/index.php?title=Guelph/12_August_2008&action=edit 12]	[http://2008.igem.org/wiki/index.php?title=Guelph/13_August_2008&action=edit 13]	[http://2008.igem.org/wiki/index.php?title=Guelph/14_August_2008&action=edit 14]	[http://2008.igem.org/Guelph/15_August_2008 15]	[http://2008.igem.org/wiki/index.php?title=Guelph/16_August_2008&action=edit 16]	[http://2008.igem.org/wiki/index.php?title=Guelph/17_August_2008&action=edit 17]
[http://2008.igem.org/wiki/index.php?title=Guelph/18_August_2008&action=edit 18]	[http://2008.igem.org/wiki/index.php?title=Guelph/19_August_2008&action=edit 19]	[http://2008.igem.org/wiki/index.php?title=Guelph/20_August_2008&action=edit 20]	[http://2008.igem.org/wiki/index.php?title=Guelph/21_August_2008&action=edit 21]	[http://2008.igem.org/wiki/index.php?title=Guelph/22_August_2008&action=edit 22]	[http://2008.igem.org/wiki/index.php?title=Guelph/23_August_2008&action=edit 23]	[http://2008.igem.org/wiki/index.php?title=Guelph/24_August_2008&action=edit 24]
[http://2008.igem.org/wiki/index.php?title=Guelph/25_August_2008&action=edit 25]	[http://2008.igem.org/wiki/index.php?title=Guelph/26_August_2008&action=edit 26]	[http://2008.igem.org/wiki/index.php?title=Guelph/27_August_2008&action=edit 27]	[http://2008.igem.org/wiki/index.php?title=Guelph/28_August_2008&action=edit 28]	[http://2008.igem.org/wiki/index.php?title=Guelph/29_August_2008&action=edit 29]	[http://2008.igem.org/wiki/index.php?title=Guelph/30_August_2008&action=edit 30]	[http://2008.igem.org/wiki/index.php?title=Guelph/31_August_2008&action=edit 31]

September
M	T	W	T	F	S	S
[http://2008.igem.org/Guelph/1_September_2008 1]	[http://2008.igem.org/wiki/index.php?title=Guelph/2_September_2008&action=edit 2]	[http://2008.igem.org/wiki/index.php?title=Guelph/3_September_2008&action=edit 3]	[http://2008.igem.org/wiki/index.php?title=Guelph/4_September_2008&action=edit 4]	[http://2008.igem.org/wiki/index.php?title=Guelph/5_September_2008&action=edit 5]	[http://2008.igem.org/wiki/index.php?title=Guelph/6_September_2008&action=edit 6]	[http://2008.igem.org/wiki/index.php?title=Guelph/7_September_2008&action=edit 7]
[http://2008.igem.org/wiki/index.php?title=Guelph/8_September_2008&action=edit 8]	[http://2008.igem.org/wiki/index.php?title=Guelph/9_September_2008&action=edit 9]	[http://2008.igem.org/wiki/index.php?title=Guelph/10_September_2008&action=edit 10]	[http://2008.igem.org/wiki/index.php?title=Guelph/11_September_2008&action=edit 11]	[http://2008.igem.org/wiki/index.php?title=Guelph/12_September_2008&action=edit 12]	[http://2008.igem.org/wiki/index.php?title=Guelph/13_September_2008&action=edit 13]	[http://2008.igem.org/wiki/index.php?title=Guelph/14_September_2008&action=edit 14]
[http://2008.igem.org/wiki/index.php?title=Guelph/15_September_2008&action=edit 15]	[http://2008.igem.org/wiki/index.php?title=Guelph/16_September_2008&action=edit 16]	[http://2008.igem.org/Guelph/17_September_2008 17]	[http://2008.igem.org/wiki/index.php?title=Guelph/18_September_2008&action=edit 18]	[http://2008.igem.org/wiki/index.php?title=Guelph/19_September_2008&action=edit 19]	[http://2008.igem.org/wiki/index.php?title=Guelph/20_September_2008&action=edit 20]	[http://2008.igem.org/wiki/index.php?title=Guelph/21_September_2008&action=edit 21]
[http://2008.igem.org/Guelph/22_September_2008 22]	[http://2008.igem.org/wiki/index.php?title=Guelph/23_September_2008&action=edit 23]	[http://2008.igem.org/wiki/index.php?title=Guelph/24_September_2008&action=edit 24]	[http://2008.igem.org/wiki/index.php?title=Guelph/25_September_2008&action=edit 25]	[http://2008.igem.org/wiki/index.php?title=Guelph/26_September_2008&action=edit 26]	[http://2008.igem.org/wiki/index.php?title=Guelph/27_September_2008&action=edit 27]	[http://2008.igem.org/wiki/index.php?title=Guelph/28_September_2008&action=edit 28]
[http://2008.igem.org/wiki/index.php?title=Guelph/29_September_2008&action=edit 29]	[http://2008.igem.org/wiki/index.php?title=Guelph/30_September_2008&action=edit 30]

October
M	T	W	T	F	S	S
		[http://2008.igem.org/Guelph/1_October_2008 1]	[http://2008.igem.org/Guelph/2_October_2008 2]	[http://2008.igem.org/wiki/index.php?title=Guelph/3_October_2008&action=edit 3]	[http://2008.igem.org/wiki/index.php?title=Guelph/4_October_2008&action=edit 4]	[http://2008.igem.org/wiki/index.php?title=Guelph/5_October_2008&action=edit 5]
[http://2008.igem.org/wiki/index.php?title=Guelph/6_October_2008&action=edit 6]	[http://2008.igem.org/Guelph/7_October_2008 7]	[http://2008.igem.org/wiki/index.php?title=Guelph/8_October_2008&action=edit 8]	[http://2008.igem.org/Guelph/9_October_2008 9]	[http://2008.igem.org/wiki/index.php?title=Guelph/10_October_2008&action=edit 10]	[http://2008.igem.org/wiki/index.php?title=Guelph/11_October_2008&action=edit 11]	[http://2008.igem.org/wiki/index.php?title=Guelph/12_October_2008&action=edit 12]
[http://2008.igem.org/wiki/index.php?title=Guelph/13_October_2008&action=edit 13]	[http://2008.igem.org/wiki/index.php?title=Guelph/14_October_2008&action=edit 14]	[http://2008.igem.org/Guelph/15_October_2008 15]	[http://2008.igem.org/wiki/index.php?title=Guelph/16_October_2008&action=edit 16]	[http://2008.igem.org/Guelph/17_October_2008 17]	[http://2008.igem.org/wiki/index.php?title=Guelph/18_October_2008&action=edit 18]	[http://2008.igem.org/wiki/index.php?title=Guelph/19_October_2008&action=edit 19]
[http://2008.igem.org/wiki/index.php?title=Guelph/20_October_2008&action=edit 20]	[http://2008.igem.org/Guelph/21_October_2008 21]	[http://2008.igem.org/wiki/index.php?title=Guelph/22_October_2008&action=edit 22]	[http://2008.igem.org/wiki/index.php?title=Guelph/23_October_2008&action=edit 23]	[http://2008.igem.org/wiki/index.php?title=Guelph/24_October_2008&action=edit 24]	[http://2008.igem.org/wiki/index.php?title=Guelph/25_October_2008&action=edit 25]	[http://2008.igem.org/wiki/index.php?title=Guelph/26_October_2008&action=edit 26]
[http://2008.igem.org/wiki/index.php?title=Guelph/27_October_2008&action=edit 27]	[http://2008.igem.org/wiki/index.php?title=Guelph/28_October_2008&action=edit 28]	[http://2008.igem.org/Guelph/29_October_2008 29]	[http://2008.igem.org/wiki/index.php?title=Guelph/30_October_2008&action=edit 30]	[http://2008.igem.org/wiki/index.php?title=Guelph/31_October_2008&action=edit 31]

November
M	T	W	T	F	S	S
					[http://2008.igem.org/wiki/index.php?title=Guelph/1_November_2008&action=edit 1]	[http://2008.igem.org/wiki/index.php?title=Guelph/2_November_2008&action=edit 2]
[http://2008.igem.org/wiki/index.php?title=Guelph/3_November_2008&action=edit 3]	[http://2008.igem.org/wiki/index.php?title=Guelph/4_November_2008&action=edit 4]	[http://2008.igem.org/wiki/index.php?title=Guelph/5_November_2008&action=edit 5]	[http://2008.igem.org/wiki/index.php?title=Guelph/6_November_2008&action=edit 6]	[http://2008.igem.org/wiki/index.php?title=Guelph/7_November_2008&action=edit 7]	[http://2008.igem.org/Guelph/8_November_2008 8]	[http://2008.igem.org/wiki/index.php?title=Guelph/9_November_2008&action=edit 9]
[http://2008.igem.org/wiki/index.php?title=Guelph/10_November_2008&action=edit 10]	[http://2008.igem.org/wiki/index.php?title=Guelph/11_November_2008&action=edit 11]	[http://2008.igem.org/wiki/index.php?title=Guelph/12_November_2008&action=edit 12]	[http://2008.igem.org/wiki/index.php?title=Guelph/13_November_2008&action=edit 13]	[http://2008.igem.org/wiki/index.php?title=Guelph/14_November_2008&action=edit 14]	[http://2008.igem.org/wiki/index.php?title=Guelph/15_November_2008&action=edit 15]	[http://2008.igem.org/wiki/index.php?title=Guelph/16_November_2008&action=edit 16]
[http://2008.igem.org/wiki/index.php?title=Guelph/17_November_2008&action=edit 17]	[http://2008.igem.org/wiki/index.php?title=Guelph/18_November_2008&action=edit 18]	[http://2008.igem.org/wiki/index.php?title=Guelph/19_November_2008&action=edit 19]	[http://2008.igem.org/wiki/index.php?title=Guelph/20_November_2008&action=edit 20]	[http://2008.igem.org/wiki/index.php?title=Guelph/21_November_2008&action=edit 21]	[http://2008.igem.org/wiki/index.php?title=Guelph/22_November_2008&action=edit 22]	[http://2008.igem.org/wiki/index.php?title=Guelph/23_November_2008&action=edit 23]
[http://2008.igem.org/wiki/index.php?title=Guelph/24_November_2008&action=edit 24]	[http://2008.igem.org/wiki/index.php?title=Guelph/25_November_2008&action=edit 25]	[http://2008.igem.org/wiki/index.php?title=Guelph/26_November_2008&action=edit 26]	[http://2008.igem.org/wiki/index.php?title=Guelph/27_November_2008&action=edit 27]	[http://2008.igem.org/wiki/index.php?title=Guelph/28_November_2008&action=edit 28]	[http://2008.igem.org/wiki/index.php?title=Guelph/29_November_2008&action=edit 29]	[http://2008.igem.org/wiki/index.php?title=Guelph/30_November_2008&action=edit 30]

@@ Line 22: / Line 22: @@
 <h3>To Do List</h3>
+-redo PCR of CrtI with giant primers to eliminate PstI sites and cut the PCR product with PstI to select only for "fixed" fragments (Dave and Lisa)
 -cut and ligate the synthetic operon now that we have the genes cloned (Lisa, Ed, Brendan)