http://2008.igem.org/wiki/index.php?title=Team:Harvard/Dailybook/Week0&feed=atom&action=history
Team:Harvard/Dailybook/Week0 - Revision history
2024-03-29T15:52:13Z
Revision history for this page on the wiki
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http://2008.igem.org/wiki/index.php?title=Team:Harvard/Dailybook/Week0&diff=80028&oldid=prev
Joyy at 17:34, 28 October 2008
2008-10-28T17:34:20Z
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<td colspan='2' style="background-color: white; color:black;">Revision as of 17:34, 28 October 2008</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*GFP/OD Measurement ([[Team:Harvard/PrelimTest#OD_and_GFP_expression_in_E._Coli|results]])</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*GFP/OD Measurement ([[Team:Harvard/PrelimTest#OD_and_GFP_expression_in_E._Coli|results]])</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Make glycerol stocks</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Make glycerol stocks</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Miniprep vectors ([[Team:Harvard<del class="diffchange diffchange-inline">/Notebook</del>/GenProtocols#QIAprep_Spin_Miniprep_Kit|protocol]])</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Miniprep vectors ([[Team:Harvard/GenProtocols#QIAprep_Spin_Miniprep_Kit|protocol]])</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Make competent Shewie</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Make competent Shewie</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>**Chemically competent ([[Team:Harvard<del class="diffchange diffchange-inline">/Notebook</del>/GenProtocols#Chemically_competent_cells|protocol]]) - actually used Zymo protocol which didn't work for Shewie and I don't think for E. coli</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>**Chemically competent ([[Team:Harvard/GenProtocols#Chemically_competent_cells|protocol]]) - actually used Zymo protocol which didn't work for Shewie and I don't think for E. coli</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>**Electrocompetent ([[Team:Harvard<del class="diffchange diffchange-inline">/Notebooks</del>/GenProtocols#For_electrocompetent_cells|protocol]]) - actually used earlier protocol w/o sorbitol</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>**Electrocompetent ([[Team:Harvard/GenProtocols#For_electrocompetent_cells|protocol]]) - actually used earlier protocol w/o sorbitol</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Pour Cm plates</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Pour Cm plates</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Transform Shewie with pACYC Duet and GFP vectors</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Transform Shewie with pACYC Duet and GFP vectors</div></td></tr>
</table>
Joyy
http://2008.igem.org/wiki/index.php?title=Team:Harvard/Dailybook/Week0&diff=80010&oldid=prev
Joyy at 17:33, 28 October 2008
2008-10-28T17:33:06Z
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<td colspan='2' style="background-color: white; color:black;">Revision as of 17:33, 28 October 2008</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*GFP/OD Measurement ([[Team:Harvard/PrelimTest#OD_and_GFP_expression_in_E._Coli|results]])</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*GFP/OD Measurement ([[Team:Harvard/PrelimTest#OD_and_GFP_expression_in_E._Coli|results]])</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Make glycerol stocks</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Make glycerol stocks</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Miniprep vectors ([[<del class="diffchange diffchange-inline">IGEM</del>:Harvard/<del class="diffchange diffchange-inline">2008/Lab_Notebooks</del>/GenProtocols#QIAprep_Spin_Miniprep_Kit|protocol]])</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Miniprep vectors ([[<ins class="diffchange diffchange-inline">Team</ins>:Harvard/<ins class="diffchange diffchange-inline">Notebook</ins>/GenProtocols#QIAprep_Spin_Miniprep_Kit|protocol]])</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Make competent Shewie</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Make competent Shewie</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>**Chemically competent ([[<del class="diffchange diffchange-inline">IGEM</del>:Harvard/<del class="diffchange diffchange-inline">2008/Lab_Notebooks</del>/GenProtocols#Chemically_competent_cells|protocol]]) - actually used Zymo protocol which didn't work for Shewie and I don't think for E. coli</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>**Chemically competent ([[<ins class="diffchange diffchange-inline">Team</ins>:Harvard/<ins class="diffchange diffchange-inline">Notebook</ins>/GenProtocols#Chemically_competent_cells|protocol]]) - actually used Zymo protocol which didn't work for Shewie and I don't think for E. coli</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>**Electrocompetent ([[<del class="diffchange diffchange-inline">IGEM</del>:Harvard/<del class="diffchange diffchange-inline">2008/Lab_Notebooks</del>/GenProtocols#For_electrocompetent_cells|protocol]]) - actually used earlier protocol w/o sorbitol</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>**Electrocompetent ([[<ins class="diffchange diffchange-inline">Team</ins>:Harvard/<ins class="diffchange diffchange-inline">Notebooks</ins>/GenProtocols#For_electrocompetent_cells|protocol]]) - actually used earlier protocol w/o sorbitol</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Pour Cm plates</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Pour Cm plates</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Transform Shewie with pACYC Duet and GFP vectors</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Transform Shewie with pACYC Duet and GFP vectors</div></td></tr>
</table>
Joyy
http://2008.igem.org/wiki/index.php?title=Team:Harvard/Dailybook/Week0&diff=80003&oldid=prev
Joyy at 17:32, 28 October 2008
2008-10-28T17:32:21Z
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<td colspan='2' style="background-color: white; color:black;">Revision as of 17:32, 28 October 2008</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Tuesday: June 17, 2008=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Tuesday: June 17, 2008=</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*GFP/OD Measurement ([[<del class="diffchange diffchange-inline">IGEM</del>:Harvard<del class="diffchange diffchange-inline">/2008/Lab_Notebooks</del>/PrelimTest#OD_and_GFP_expression_in_E._Coli|results]])</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*GFP/OD Measurement ([[<ins class="diffchange diffchange-inline">Team</ins>:Harvard/PrelimTest#OD_and_GFP_expression_in_E._Coli|results]])</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Make glycerol stocks</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Make glycerol stocks</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Miniprep vectors ([[IGEM:Harvard/2008/Lab_Notebooks/GenProtocols#QIAprep_Spin_Miniprep_Kit|protocol]])</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Miniprep vectors ([[IGEM:Harvard/2008/Lab_Notebooks/GenProtocols#QIAprep_Spin_Miniprep_Kit|protocol]])</div></td></tr>
</table>
Joyy
http://2008.igem.org/wiki/index.php?title=Team:Harvard/Dailybook/Week0&diff=69300&oldid=prev
Joyy: /* Transformed S1 via Electroporation */
2008-10-26T19:49:18Z
<p><span class="autocomment">Transformed S1 via Electroporation</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 19:49, 26 October 2008</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Transformed S1 via Electroporation==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Transformed S1 via Electroporation==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We used the electroporation protocol as noted in [[Team:Harvard/GenProtocols#For_electrocompetent_cells General Protocols]] with the following volumes of vector added:</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We used the electroporation protocol as noted in [[Team:Harvard/GenProtocols#For_electrocompetent_cells <ins class="diffchange diffchange-inline">| </ins>General Protocols]] with the following volumes of vector added:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* P1 (J23113 low) - 2uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* P1 (J23113 low) - 2uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* P2 (J23150 med) - 2uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* P2 (J23150 med) - 2uL</div></td></tr>
</table>
Joyy
http://2008.igem.org/wiki/index.php?title=Team:Harvard/Dailybook/Week0&diff=69298&oldid=prev
Joyy: /* Transformed S1 via Electroporation */
2008-10-26T19:48:46Z
<p><span class="autocomment">Transformed S1 via Electroporation</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 19:48, 26 October 2008</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Transformed S1 via Electroporation==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Transformed S1 via Electroporation==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We used the electroporation protocol as noted in [Team:Harvard/GenProtocols#For_electrocompetent_cells General Protocols] with the following volumes of vector added:</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We used the electroporation protocol as noted in <ins class="diffchange diffchange-inline">[</ins>[Team:Harvard/GenProtocols#For_electrocompetent_cells General Protocols<ins class="diffchange diffchange-inline">]</ins>] with the following volumes of vector added:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* P1 (J23113 low) - 2uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* P1 (J23113 low) - 2uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* P2 (J23150 med) - 2uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* P2 (J23150 med) - 2uL</div></td></tr>
</table>
Joyy
http://2008.igem.org/wiki/index.php?title=Team:Harvard/Dailybook/Week0&diff=69296&oldid=prev
Joyy: /* Transformed S1 via Electroporation */
2008-10-26T19:48:18Z
<p><span class="autocomment">Transformed S1 via Electroporation</span></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">Revision as of 19:48, 26 October 2008</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Transformed S1 via Electroporation==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Transformed S1 via Electroporation==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We used the electroporation protocol as noted in [<del class="diffchange diffchange-inline">http://openwetware.org/wiki/IGEM</del>:Harvard<del class="diffchange diffchange-inline">/2008/Lab_Notebooks</del>/GenProtocols#For_electrocompetent_cells General Protocols] with the following volumes of vector added:</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We used the electroporation protocol as noted in [<ins class="diffchange diffchange-inline">Team</ins>:Harvard/GenProtocols#For_electrocompetent_cells General Protocols] with the following volumes of vector added:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* P1 (J23113 low) - 2uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* P1 (J23113 low) - 2uL</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* P2 (J23150 med) - 2uL</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* P2 (J23150 med) - 2uL</div></td></tr>
</table>
Joyy
http://2008.igem.org/wiki/index.php?title=Team:Harvard/Dailybook/Week0&diff=69293&oldid=prev
Joyy: /* Protocols */
2008-10-26T19:47:47Z
<p><span class="autocomment">Protocols</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 19:47, 26 October 2008</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>| E10 ||[http://partsregistry.org/Part:BBa_I715038 BBa_I715038]||T7 Polymerase with LacI promoter||pMB1||2878||Amp</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>| E10 ||[http://partsregistry.org/Part:BBa_I715038 BBa_I715038]||T7 Polymerase with LacI promoter||pMB1||2878||Amp</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|} </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|} </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>:::'''For list of all parts, see [[<del class="diffchange diffchange-inline">IGEM</del>:Harvard<del class="diffchange diffchange-inline">/2008</del>/Plasmids#Parts|here]].'''</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>:::'''For list of all parts, see [[<ins class="diffchange diffchange-inline">Team</ins>:Harvard/Plasmids#Parts|here]].'''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*transform into E. coli TOP10 cells</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:*transform into E. coli TOP10 cells</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Make chemically and electrocompetent Shewie MR-1</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*Make chemically and electrocompetent Shewie MR-1</div></td></tr>
</table>
Joyy
http://2008.igem.org/wiki/index.php?title=Team:Harvard/Dailybook/Week0&diff=69291&oldid=prev
Joyy: /* Tuesday: June 17, 2008 */
2008-10-26T19:47:30Z
<p><span class="autocomment">Tuesday: June 17, 2008</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 19:47, 26 October 2008</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>| E3b || [http://partsregistry.org/Part:BBa_J23113 J23151]||68.5</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>| E3b || [http://partsregistry.org/Part:BBa_J23113 J23151]||68.5</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>::'''For list of all parts, see [[<del class="diffchange diffchange-inline">IGEM</del>:Harvard<del class="diffchange diffchange-inline">/2008</del>/Plasmids#Parts|here]].'''</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>::'''For list of all parts, see [[<ins class="diffchange diffchange-inline">Team</ins>:Harvard/Plasmids#Parts|here]].'''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Wednesday: June 18, 2008=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Wednesday: June 18, 2008=</div></td></tr>
</table>
Joyy
http://2008.igem.org/wiki/index.php?title=Team:Harvard/Dailybook/Week0&diff=69289&oldid=prev
Joyy: /* Tuesday: June 17, 2008 */
2008-10-26T19:47:02Z
<p><span class="autocomment">Tuesday: June 17, 2008</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>| E4 || [http://www.merckbiosciences.co.uk/html/NVG/duettable.html pACYC duet]||Low-Medium copy vector||p15a||4008||Cm</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>| E4 || [http://www.merckbiosciences.co.uk/html/NVG/duettable.html pACYC duet]||Low-Medium copy vector||p15a||4008||Cm</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>::'''For list of all parts, see [[<del class="diffchange diffchange-inline">IGEM</del>:Harvard<del class="diffchange diffchange-inline">/2008</del>/Plasmids#Parts|here]].'''</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>::'''For list of all parts, see [[<ins class="diffchange diffchange-inline">Team</ins>:Harvard/Plasmids#Parts|here]].'''</div></td></tr>
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Joyy
http://2008.igem.org/wiki/index.php?title=Team:Harvard/Dailybook/Week0&diff=69041&oldid=prev
Joyy at 18:50, 26 October 2008
2008-10-26T18:50:58Z
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">{|</del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><!--- body here---></del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Monday: June 16, 2008=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Monday: June 16, 2008=</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>| 7700 || Model 7700 20-Channel, Differential Multiplexer Module w/ Automatic CJC, Screw Terminals, and up to 50MHz Bandwidth (for Models 2700, 2701, and 2750) </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>| 7700 || Model 7700 20-Channel, Differential Multiplexer Module w/ Automatic CJC, Screw Terminals, and up to 50MHz Bandwidth (for Models 2700, 2701, and 2750) </div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><!--- end body ---></del></div></td><td colspan="2"> </td></tr>
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Joyy