http://2008.igem.org/wiki/index.php?title=Team:Harvard/Dailybook/Week14/Chemical_and_Light&feed=atom&action=history
Team:Harvard/Dailybook/Week14/Chemical and Light - Revision history
2024-03-29T08:35:01Z
Revision history for this page on the wiki
MediaWiki 1.16.5
http://2008.igem.org/wiki/index.php?title=Team:Harvard/Dailybook/Week14/Chemical_and_Light&diff=69203&oldid=prev
Joyy: New page: =Mystery Colony PCR 10/08= Colony PCR of putative fungal colonies using primers BBpfx and mtrB500. Image:10-08_Mystery_PCR_MXHTA.jpg Lane 1: 1 kb ladder Lanes 2-6: P136 plate (500 b...
2008-10-26T19:22:56Z
<p>New page: =Mystery Colony PCR 10/08= Colony PCR of putative fungal colonies using primers BBpfx and mtrB500. <a href="/File:10-08_Mystery_PCR_MXHTA.jpg" title="File:10-08 Mystery PCR MXHTA.jpg">Image:10-08_Mystery_PCR_MXHTA.jpg</a> Lane 1: 1 kb ladder Lanes 2-6: P136 plate (500 b...</p>
<p><b>New page</b></p><div>=Mystery Colony PCR 10/08=<br />
Colony PCR of putative fungal colonies using primers BBpfx and mtrB500.<br />
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[[Image:10-08_Mystery_PCR_MXHTA.jpg]]<br />
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Lane 1: 1 kb ladder<br />
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Lanes 2-6: P136 plate (500 bp)<br />
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Lane 7: P140 plate (1.9 kb)<br />
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Lanes 8-12: P122>131 plate (1.3 kb)<br />
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=Colony PCR 10/11=<br />
Unadulterated gel<br />
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[[Image:10-11_Colony_PCR_MXHTA.jpg]]<br />
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[[Image:10-11_Colony_PCR_1-12.jpg]]<br />
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Lanes 1-2: P148<br />
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Lane 3-4: P147 (picked 3)<br />
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Lanes 5-12: 147 (picked 6)<br />
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[[Image:10-11_Colony_PCR_13-24.jpg]]<br />
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Lanes 13-14: P147<br />
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Lanes 15-24: P146 (picked 17, 23)<br />
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[[Image:10-11_Colony_PCR_25-36.jpg]]<br />
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Lanes 25-26: P146<br />
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Lanes 27-36: P144 (picked 30, 35)<br />
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[[Image:10-11_Colony_PCR_37-48.jpg]]<br />
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Lanes 37-39: P144<br />
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Lanes 40-48: P145 (picked 41, 46)<br />
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=Ligations 10/10=<br />
{| {{table}}<br />
| align="center" style="background:#f0f0f0;"|''''''<br />
| align="center" style="background:#f0f0f0;"|'''Part 1'''<br />
| align="center" style="background:#f0f0f0;"|'''Part 2'''<br />
| align="center" style="background:#f0f0f0;"|'''Part 3'''<br />
| align="center" style="background:#f0f0f0;"|'''Notes'''<br />
| align="center" style="background:#f0f0f0;"|''''''<br />
| align="center" style="background:#f0f0f0;"|'''Total Volume'''<br />
| align="center" style="background:#f0f0f0;"|'''Growth'''<br />
|-<br />
| Ligation 1||123m4 ES||131 XP||P1 EP||ts in Kan||just chem tran||||<br />
|-<br />
| ||8||4||3||||||15||<br />
|-<br />
| Ligation 2||122m4 Es||131 XP||P1 EP||ts in kan||just chem tran||||<br />
|-<br />
| ||8||4||3||||||15||<br />
|-<br />
| Ligation 3||38+130 #20 ESX mmI||P1 ES||||||||||<br />
|-<br />
| ||||2ul to tube||||||||3||<br />
|-<br />
| Ligation 4||39+130 #31 ESX ||P1 ES||||||||||<br />
|-<br />
| ||||10ul to insert tube||||||||35||<br />
|-<br />
| Ligation 5||142 Es||P1 ES||||||||<br />
|-<br />
| ||||10ul to insert tube||||||||23<br />
|-<br />
| Ligation 6||138 #2 ES||P1 ES||||||||<br />
|-<br />
| ||||10ul to insert tube||||||||20<br />
|-<br />
| Ligation 7||137 #32 ES||P1 ES||||||||<br />
|-<br />
| ||||10ul to insert tube||||||||31<br />
|-<br />
| Ligation 8||140 ES||P1 ES||||||||<br />
|-<br />
| ||||10ul to insert tube||||||||20<br />
|-<br />
| Ligation 9||131 XP||116 SP||||||||<br />
|-<br />
| ||4||4||||||||8<br />
|-<br />
| Ligation 10||131 XP||117 SP||||||||<br />
|-<br />
| ||4||4||||||||8<br />
|-<br />
| Ligation 11||131 XP||108 Sp||||||||<br />
|-<br />
| ||4||4||||||||8<br />
|}</div>
Joyy