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Team:Harvard/Dailybook/Week7/Widgetry
From 2008.igem.org
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Linchinlee (Talk | contribs) (→Monday: August 4, 2008) |
Linchinlee (Talk | contribs) (→Co-culture experiment setup) |
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== Co-culture experiment setup == | == Co-culture experiment setup == | ||
| + | |||
| + | * Chambers 1 & 2: wt E. coli + wt Shewie + lactose | ||
| + | * Chambers 4 &5 : Lac-operon knockout E. coli + wt Shewie + lactose | ||
| + | |||
| + | '''Positive Controls''' | ||
| + | * Chamber 3: wt E. coli + wt Shewie + lactate | ||
| + | * Chamber 6: Lac-operon knockout E. coli + wt Shewie + lactate | ||
| + | |||
| + | '''Negative Controls''' | ||
| + | * Chamber 7: wt Shewie + lactose | ||
| + | * Chamber 8: wt E. coli + lactose | ||
| + | * Chamber 9: Lac-operon knockout E. coli + lactose | ||
=Tuesday: August 5, 2008= | =Tuesday: August 5, 2008= | ||
Revision as of 21:54, 26 October 2008
Contents |
Goals for this week
- Shewanella and E. coli co-culture experiments
- If Shewanella responds rapidly to lactate, and E. coli breaks down lactose into lactate, can we couple lactose breakdown by E. coli with current production by Shewanella?
- Motivation:
- An inducible system in E. coli could be used to control Shewanella current production
Monday: August 4, 2008
Co-culture experiment setup
- Chambers 1 & 2: wt E. coli + wt Shewie + lactose
- Chambers 4 &5 : Lac-operon knockout E. coli + wt Shewie + lactose
Positive Controls
- Chamber 3: wt E. coli + wt Shewie + lactate
- Chamber 6: Lac-operon knockout E. coli + wt Shewie + lactate
Negative Controls
- Chamber 7: wt Shewie + lactose
- Chamber 8: wt E. coli + lactose
- Chamber 9: Lac-operon knockout E. coli + lactose
