Team:Harvard/Dailybook/Week7/Widgetry

From 2008.igem.org

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(Tuesday: August 5, 2008)
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==Co-culture experiment results, day 1==
==Co-culture experiment results, day 1==
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[[Image: Co-culture_part1.JPG | 700 px]]
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[[Image: Co-culture_part1_zoomed.jpg | 700 px]]
=Wednesday: August 6, 2008=
=Wednesday: August 6, 2008=
=Thursday: August 7, 2008=
=Thursday: August 7, 2008=
=Friday: August 8, 2008=
=Friday: August 8, 2008=

Revision as of 21:58, 26 October 2008

Contents

Goals for this week

  • Shewanella and E. coli co-culture experiments
    • If Shewanella responds rapidly to lactate, and E. coli breaks down lactose into lactate, can we couple lactose breakdown by E. coli with current production by Shewanella?
    • Motivation:
      • An inducible system in E. coli could be used to control Shewanella current production

Monday: August 4, 2008

Co-culture experiment setup

  • Chambers 1 & 2: wt E. coli + wt Shewie + lactose
  • Chambers 4 &5 : Lac-operon knockout E. coli + wt Shewie + lactose

Positive Controls

  • Chamber 3: wt E. coli + wt Shewie + lactate
  • Chamber 6: Lac-operon knockout E. coli + wt Shewie + lactate

Negative Controls

  • Chamber 7: wt Shewie + lactose
  • Chamber 8: wt E. coli + lactose
  • Chamber 9: Lac-operon knockout E. coli + lactose

Tuesday: August 5, 2008

Co-culture experiment results, day 1

Co-culture part1.JPG

Co-culture part1 zoomed.jpg

Wednesday: August 6, 2008

Thursday: August 7, 2008

Friday: August 8, 2008