Team:Harvard/Hardware/MFCProcedure

From 2008.igem.org

(Difference between revisions)
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* Spiral Point Tap 1/4"-28
* Spiral Point Tap 1/4"-28
* 8 Plastic Luer Lock Coupling Nylon, Female to Male Thread, 1/4"-28
* 8 Plastic Luer Lock Coupling Nylon, Female to Male Thread, 1/4"-28
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* 8 Luer Lock Injection Ports
Procedure
Procedure
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#* Attach 2700 to COM1 port of desktop computer w/ LabVIEW™
#* Attach 2700 to COM1 port of desktop computer w/ LabVIEW™
#* Download our LabVIEW™ source code [[Media:MFCs.vi|MFCs.vi]]
#* Download our LabVIEW™ source code [[Media:MFCs.vi|MFCs.vi]]
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#* Open Program in LabVIEW™,  
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#* Open Program in LabVIEW™, adjust block diagram as necessary
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#*
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==Experiment Preparation==
==Experiment Preparation==
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===Solutions Prep===
===Solutions Prep===
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Chamber media
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# Chamber media
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* 5.844 g/L 100mM NaCl
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#* 5.844 g/L 100mM NaCl
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* 15.1185 g/L 50mM PIPES (hydrogen)  
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#* 15.1185 g/L 50mM PIPES (hydrogen)  
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''7.0 pH''
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#* ''7.0 pH''
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Phosphate buffer
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# Phosphate buffer
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* 2.918 g/L Monosodium phosphate, monohydrate
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#* 2.918 g/L Monosodium phosphate, monohydrate
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* 4.095 g/L Disodium phosphate, anhydrous   
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#* 4.095 g/L Disodium phosphate, anhydrous   
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* 5.844 g/L 100mM NaCl
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#* 5.844 g/L 100mM NaCl
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''7.0 pH''
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#* ''7.0 pH''
===Gas Tubing Assembly===
===Gas Tubing Assembly===

Revision as of 01:51, 30 October 2008



Running an MFC Experiment

This page is intended as a comprehensive guide to completing a microbial fuel cell setup and running an experiment from start to finish.

Creating a Testing Environment

Begin 1-2 weeks prior to experiment

Constructing Fuel Cell Components

Materials (per fuel cell)

  • 4" Polycarbonate Square Tube, 2" Outer Diameter
  • 6" x 6" Polycarbonate Sheet, 1/4" Thick
  • 4 Steel Fully Threaded Stud, 1/4"-20 Thread, 6" Length
  • 8 Zinc Alloy Wing Flange Nut, 1/4"-20 Screw Size, 1" Wing Spread
  • 1" x 1" Nafion® membrane, 0.180mm thick
  • 1" x 1" Carbon felt, 0.25" thick
  • 1.5" x 1.5" E-TEK ELAT™ GDE (platinum on carbon)
  • 2' Titanium Grade 2 Wire .046" Diameter
  • Teflon Tape, 1/4" Width
  • 5" x 2.5" Silicone Sheet
  • Silicone Glue
  • Spiral Point Tap 1/4"-28
  • 8 Plastic Luer Lock Coupling Nylon, Female to Male Thread, 1/4"-28
  • 8 Luer Lock Injection Ports

Procedure

  1. Mill Polycarbonate
    • Cut polycarbonate sheet into 4 equal 3" x 3" pieces
    • Drill four 3/8" holes through each piece, 1 per corner, indented 5mm from both sides

Endplates.jpg

    • Cut polycarbonate tube into two equal 2" halves
    • Drill four 1/4" holes through each half in configuration shown

Drilled tube.jpg

    • Tap each hole with 1/4" -28 spiral tap
  1. Glue Chambers (repeat for each half)
    • Center tube on endplate by marking plate with 'X' from corner to corner
    • Squirt 2mm thick line of silicone on edge of tube (edge furthest from holes)
    • Press tube firmly against marked location on endplate
    • Quickly spread excess silicone along edge
    • Let stand 24h to harden

Glued half.jpg

  1. Construct Gaskets
    • Cut silicone sheet into two equal 2.25" x 2.25" pieces
    • Cut out centered inner squares in each piece, 1.75" x 1.75"
    • Using inner squares, cut two 'O' rings, inner diameter 1/4", outer diameter 1/2"

Gaskets.jpg

  1. Construct Electrodes
    • Cut titanium wire into one 8" piece and one 16" piece
    • Using pliers, shape anode and cathode as shown

600px

    • Spear carbon felt with tip of anode titanium wire and wedge into frame
    • Weave platinum carbon cloth through cathode titanium wire

600px

  1. Seal Injection Ports
    • Wrap threads of all eight Luer Lock screws with 1' of teflon tape in opposite direction of screwing
    • Screw Luer Locks into all tapped holes in both chambers

Fin chamber.jpg


Setup of Measurement Device

Materials

  • Keithley 2700 Digital Multimeter
  • Keithley 7700 Multiplexer
  • Small Breadboard
  • Supply of insulted thin copper wire
  • 470 Ohm resistors (one/fuel cell)

Procedure

  1. Wire Multiplexer
    • Open multiplexer, note channels
    • Cut two wire 18" wire leads per fuel cell
    • Strip ends, place one wire in each screw terminal, screw tight
    • Tape paired wires (two are attached to each channel) near non-attached ends and label
    • Clamp wire bundles near back of device with provided plastic latch clamps
    • Close Multiplexer and slide into 2700 DMM
  1. Create Resistor Array
    • Connect resistors across middle of breadboard (one per fuel cell)
    • Connect leads from multiplexer across resistors (one pair across each resistor)


Controlling the DMM with LabVIEW™

  1. Initialize Multimeter
    • Attach 2700 to COM1 port of desktop computer w/ LabVIEW™
    • Download our LabVIEW™ source code MFCs.vi
    • Open Program in LabVIEW™, adjust block diagram as necessary

Experiment Preparation

Begin 1 day prior to experiment

Assembling Chambers

Procedure

  1. Prepare Electrodes
    • Attach Luer Lock injection ports to all chamber screws
    • Poke tip of electrodes through designated ports from the inside

Chamber w elec.jpg

  1. Align Chambers
    • Lay one chamber on a flat surface
    • Place silicone square ring on top edge of tube
    • Place polycarbonate square on silicone
    • Place silicone 'O' ring around central pore
    • Place Nafion membrane on top of 'O' ring
    • Sandwich membrane between second 'O' ring
    • Align second polycarbonate square on top of 'O' ring
    • Center second silicone square ring on polycarbonate square
    • Set second chamber on top of silicone, ensuring ports facing same direction as first chamber
  1. Clamp Chambers
    • Move assembly into vice or clamp
    • Insert rods through holes in end plates and screw on wing nuts
    • Tighten evenly

Fuelcells.jpg

Solutions Prep

  1. Chamber media
    • 5.844 g/L 100mM NaCl
    • 15.1185 g/L 50mM PIPES (hydrogen)
    • 7.0 pH
  1. Phosphate buffer
    • 2.918 g/L Monosodium phosphate, monohydrate
    • 4.095 g/L Disodium phosphate, anhydrous
    • 5.844 g/L 100mM NaCl
    • 7.0 pH

Gas Tubing Assembly

Growing Strains

Runtime

Begin 2 hours prior to experiment

Bacteria

Fuel Cells

Injections/Variables

Clean Up