http://2008.igem.org/wiki/index.php?title=Team:Harvard/Parts/LacI&feed=atom&action=historyTeam:Harvard/Parts/LacI - Revision history2024-03-28T14:55:51ZRevision history for this page on the wikiMediaWiki 1.16.5http://2008.igem.org/wiki/index.php?title=Team:Harvard/Parts/LacI&diff=105075&oldid=prevMamut: /* LacI Inducible System */2008-10-30T04:53:39Z<p><span class="autocomment">LacI Inducible System</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 04:53, 30 October 2008</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In this system, the lac repressor (LacI) is controlled by a strong constitutive promoter, and is upstream of mtrB under the control of pLac, a LacI regulated promoter. In the default state, LacI is expressed, and inhibits transcription at pLac. This should allow us to control the expression of mtrB. In the default state, mtrB is not expressed. IPTG (an analog of allolactose) induces mtrB expression by binding to LacI, thereby preventing it from inhibiting transcription at pLac.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In this system, the lac repressor (LacI) is controlled by a strong constitutive promoter, and is upstream of mtrB under the control of pLac, a LacI regulated promoter. In the default state, LacI is expressed, and inhibits transcription at pLac. This should allow us to control the expression of mtrB. In the default state, mtrB is not expressed. IPTG (an analog of allolactose) induces mtrB expression by binding to LacI, thereby preventing it from inhibiting transcription at pLac.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div style="text-indent:0pt;color:black">[[Image:BBa_K098984.png|thumb|650px|center|BBa_K098984 with BioBrick Prefix and Suffix, an example of the circuity we engineered. BBa_K098983 is similar, with a weaker promoter driving lacI expression.]]</div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div style="text-indent:0pt;color:black">[[Image:BBa_K098984.png|thumb|650px|center|<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_K098984 </ins>BBa_K098984<ins class="diffchange diffchange-inline">] </ins>with BioBrick Prefix and Suffix, an example of the circuity we engineered. <ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_K098983 </ins>BBa_K098983<ins class="diffchange diffchange-inline">] </ins>is similar, with a weaker promoter driving lacI expression.]]</div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We didn't have enough time to finish thoroughly testing the above system, as the cloning of mtrB (toxic in ''E. coli'') was rather difficult. Our preliminary findings are quite interesting, though, so check out our [[Team:Harvard/Project| Project page]].</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We didn't have enough time to finish thoroughly testing the above system, as the cloning of mtrB (toxic in ''E. coli'') was rather difficult. Our preliminary findings are quite interesting, though, so check out our [[Team:Harvard/Project| Project page]].</div></td></tr>
</table>Mamuthttp://2008.igem.org/wiki/index.php?title=Team:Harvard/Parts/LacI&diff=105023&oldid=prevMamut: /* LacI Inducible System */2008-10-30T04:48:56Z<p><span class="autocomment">LacI Inducible System</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="text-indent:0pt;color:black">[[Image:BBa_K098984.png|thumb|650px|center|BBa_K098984 with BioBrick Prefix and Suffix, an example of the circuity we engineered. BBa_K098983 is similar, with a weaker promoter driving lacI expression.]]</div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="text-indent:0pt;color:black">[[Image:BBa_K098984.png|thumb|650px|center|BBa_K098984 with BioBrick Prefix and Suffix, an example of the circuity we engineered. BBa_K098983 is similar, with a weaker promoter driving lacI expression.]]</div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We didn't have enough time to finish thoroughly testing the above system, as the cloning of mtrB (toxic in ''E. coli'') was rather difficult. Our preliminary findings are quite interesting, though, so check out our [Team:Harvard/Project| Project page].</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We didn't have enough time to finish thoroughly testing the above system, as the cloning of mtrB (toxic in ''E. coli'') was rather difficult. Our preliminary findings are quite interesting, though, so check out our <ins class="diffchange diffchange-inline">[</ins>[Team:Harvard/Project| Project page<ins class="diffchange diffchange-inline">]</ins>].</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Induction Test for LacI System with GFP==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Induction Test for LacI System with GFP==</div></td></tr>
</table>Mamuthttp://2008.igem.org/wiki/index.php?title=Team:Harvard/Parts/LacI&diff=105021&oldid=prevMamut: /* LacI Inducible System */2008-10-30T04:48:43Z<p><span class="autocomment">LacI Inducible System</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="text-indent:0pt;color:black">[[Image:BBa_K098984.png|thumb|650px|center|BBa_K098984 with BioBrick Prefix and Suffix, an example of the circuity we engineered. BBa_K098983 is similar, with a weaker promoter driving lacI expression.]]</div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="text-indent:0pt;color:black">[[Image:BBa_K098984.png|thumb|650px|center|BBa_K098984 with BioBrick Prefix and Suffix, an example of the circuity we engineered. BBa_K098983 is similar, with a weaker promoter driving lacI expression.]]</div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We didn't have enough time to finish thoroughly testing the above system, as the cloning of mtrB (toxic in ''E. coli'') was rather difficult. Our preliminary findings are quite interesting, though, so check out our [Team:Harvard/Project Project page].</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We didn't have enough time to finish thoroughly testing the above system, as the cloning of mtrB (toxic in ''E. coli'') was rather difficult. Our preliminary findings are quite interesting, though, so check out our [Team:Harvard/Project<ins class="diffchange diffchange-inline">| </ins>Project page].</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Induction Test for LacI System with GFP==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Induction Test for LacI System with GFP==</div></td></tr>
</table>Mamuthttp://2008.igem.org/wiki/index.php?title=Team:Harvard/Parts/LacI&diff=105013&oldid=prevMamut: /* LacI Inducible System */2008-10-30T04:48:24Z<p><span class="autocomment">LacI Inducible System</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="text-indent:0pt;color:black">[[Image:BBa_K098984.png|thumb|650px|center|BBa_K098984 with BioBrick Prefix and Suffix, an example of the circuity we engineered. BBa_K098983 is similar, with a weaker promoter driving lacI expression.]]</div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="text-indent:0pt;color:black">[[Image:BBa_K098984.png|thumb|650px|center|BBa_K098984 with BioBrick Prefix and Suffix, an example of the circuity we engineered. BBa_K098983 is similar, with a weaker promoter driving lacI expression.]]</div></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">We didn't have enough time to finish thoroughly testing the above system, as the cloning of mtrB (toxic in ''E. coli'') was rather difficult. Our preliminary findings are quite interesting, though, so check out our [Team:Harvard/Project Project page].</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Induction Test for LacI System with GFP==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Induction Test for LacI System with GFP==</div></td></tr>
</table>Mamuthttp://2008.igem.org/wiki/index.php?title=Team:Harvard/Parts/LacI&diff=104126&oldid=prevMamut: /* LacI Inducible System */2008-10-30T03:56:39Z<p><span class="autocomment">LacI Inducible System</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In this system, the lac repressor (LacI) is controlled by a strong constitutive promoter, and is upstream of mtrB under the control of pLac, a LacI regulated promoter. In the default state, LacI is expressed, and inhibits transcription at pLac. This should allow us to control the expression of mtrB. In the default state, mtrB is not expressed. IPTG (an analog of allolactose) induces mtrB expression by binding to LacI, thereby preventing it from inhibiting transcription at pLac.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In this system, the lac repressor (LacI) is controlled by a strong constitutive promoter, and is upstream of mtrB under the control of pLac, a LacI regulated promoter. In the default state, LacI is expressed, and inhibits transcription at pLac. This should allow us to control the expression of mtrB. In the default state, mtrB is not expressed. IPTG (an analog of allolactose) induces mtrB expression by binding to LacI, thereby preventing it from inhibiting transcription at pLac.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div style="text-indent:0pt;color:black">[[Image:BBa_K098984.png|thumb|650px|center|BBa_K098984 with BioBrick Prefix and Suffix<del class="diffchange diffchange-inline">]]</del>, an example of the circuity we engineered. BBa_K098983 is similar, with a weaker promoter driving lacI expression.</div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div style="text-indent:0pt;color:black">[[Image:BBa_K098984.png|thumb|650px|center|BBa_K098984 with BioBrick Prefix and Suffix, an example of the circuity we engineered. BBa_K098983 is similar, with a weaker promoter driving lacI expression.<ins class="diffchange diffchange-inline">]]</ins></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Induction Test for LacI System with GFP==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Induction Test for LacI System with GFP==</div></td></tr>
</table>Mamuthttp://2008.igem.org/wiki/index.php?title=Team:Harvard/Parts/LacI&diff=104114&oldid=prevMamut: /* LacI Inducible System */2008-10-30T03:56:27Z<p><span class="autocomment">LacI Inducible System</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In this system, the lac repressor (LacI) is controlled by a strong constitutive promoter, and is upstream of mtrB under the control of pLac, a LacI regulated promoter. In the default state, LacI is expressed, and inhibits transcription at pLac. This should allow us to control the expression of mtrB. In the default state, mtrB is not expressed. IPTG (an analog of allolactose) induces mtrB expression by binding to LacI, thereby preventing it from inhibiting transcription at pLac.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In this system, the lac repressor (LacI) is controlled by a strong constitutive promoter, and is upstream of mtrB under the control of pLac, a LacI regulated promoter. In the default state, LacI is expressed, and inhibits transcription at pLac. This should allow us to control the expression of mtrB. In the default state, mtrB is not expressed. IPTG (an analog of allolactose) induces mtrB expression by binding to LacI, thereby preventing it from inhibiting transcription at pLac.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div style="text-indent:0pt;color:black">[[Image:BBa_K098984.png|thumb|650px|center|BBa_K098984 with BioBrick Prefix and Suffix]]</div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div style="text-indent:0pt;color:black">[[Image:BBa_K098984.png|thumb|650px|center|BBa_K098984 with BioBrick Prefix and Suffix]]<ins class="diffchange diffchange-inline">, an example of the circuity we engineered. BBa_K098983 is similar, with a weaker promoter driving lacI expression.</ins></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Induction Test for LacI System with GFP==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Induction Test for LacI System with GFP==</div></td></tr>
</table>Mamuthttp://2008.igem.org/wiki/index.php?title=Team:Harvard/Parts/LacI&diff=102144&oldid=prevMamut: /* Results */2008-10-30T02:49:57Z<p><span class="autocomment">Results</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div style="text-indent:0pt;color:black">[[Image:Baseline-corrected_of_lac_system.png|720px|thumb|center|IPTG successfully induces higher levels of GFP expression in cells containing [http://partsregistry.org/wiki/index.php?title=Part:BBa_K098982 BBa_K098982]]]</div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div style="text-indent:0pt;color:black">[[Image:Baseline-corrected_of_lac_system.png|720px|thumb|center|IPTG successfully induces higher levels of GFP expression in cells containing [http://partsregistry.org/wiki/index.php?title=Part:BBa_K098982 BBa_K098982]<ins class="diffchange diffchange-inline"><br>This data is also available in [https://static.igem.org/mediawiki/2008/f/fa/Baseline-corrected_of_lac_system.pdf PDF format].</ins>]]</div></div></td></tr>
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</table>Mamuthttp://2008.igem.org/wiki/index.php?title=Team:Harvard/Parts/LacI&diff=102110&oldid=prevMamut: /* Results */2008-10-30T02:48:47Z<p><span class="autocomment">Results</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Additionally, it appears that even the uninduced state of such a system has significant expression of GFP. Tighter control may require higher levels of lacI expression. This leakiness may <del class="diffchange diffchange-inline">also </del>explain the difficulty we had in cloning mtrB (a gene toxic to ''E. coli'').</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Additionally, it appears that even the uninduced state of such a system has significant expression of GFP. Tighter control may require higher levels of lacI expression. This leakiness may explain the difficulty we had in cloning mtrB (a gene toxic to ''E. coli'').</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="text-indent:0pt;color:black">[[Image:Lac.jpg|650px|thumb|center|IPTG induction raw data]]</div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="text-indent:0pt;color:black">[[Image:Lac.jpg|650px|thumb|center|IPTG induction raw data]]</div></div></td></tr>
</table>Mamuthttp://2008.igem.org/wiki/index.php?title=Team:Harvard/Parts/LacI&diff=102084&oldid=prevMamut: /* Induction Test for LacI System with GFP */2008-10-30T02:47:35Z<p><span class="autocomment">Induction Test for LacI System with GFP</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Induction Test for LacI System with GFP==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Induction Test for LacI System with GFP==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>In preparing to make the lacI system for mtrB, we tested the IPTG inducibility of [http://partsregistry.org/wiki/index.php?title=Part:BBa_K098982 BBa_K098982] in ''E. coli''. In this system, the repressor <del class="diffchange diffchange-inline">(LacI) </del>is driven by a weak promoter.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>In preparing to make the lacI system for mtrB, we tested the IPTG inducibility of [http://partsregistry.org/wiki/index.php?title=Part:BBa_K098982 BBa_K098982] in ''E. coli''. In this system, the repressor is driven by a weak promoter.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Method===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Method===</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Results===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Results===</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Induction of GFP expression was observed at both 2 and 4 hours after adding IPTG. Levels of GFP expression in uninduced samples, however, remained relatively the same throughout the 4 hours. Meanwhile, IPTG induction was not observed in either the negative control ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K098981 BBa_K098981]) or the constitutive GFP control ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K098991 BBa_K098991]).</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Induction of GFP expression was observed at both 2 and 4 hours after adding IPTG. Levels of GFP expression in uninduced samples, however, remained relatively the same throughout the 4 hours. Meanwhile, IPTG induction was not observed in either the negative control ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K098981 BBa_K098981]) or the constitutive GFP control ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K098991 BBa_K098991])<ins class="diffchange diffchange-inline">. Note that in the graph below, it appears that such an induction method is not reliable at 6 hours</ins>.</div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><div style="text-indent:0pt;color:black">[[Image:Baseline-corrected_of_lac_system.png|720px|thumb|center|IPTG successfully induces higher levels of GFP expression in cells containing [http://partsregistry.org/wiki/index.php?title=Part:BBa_K098982 BBa_K098982]]]</div></ins></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div style="text-indent:0pt;color:black">[[Image:Lac.jpg|<del class="diffchange diffchange-inline">720px</del>|thumb|center|<del class="diffchange diffchange-inline">Lac Inducibility Results</del>]]</div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Additionally, it appears that even the uninduced state of such a system has significant expression of GFP. Tighter control may require higher levels of lacI expression. This leakiness may also explain the difficulty we had in cloning mtrB (a gene toxic to ''E. coli'').</ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div style="text-indent:0pt;color:black">[[Image:Lac.jpg|<ins class="diffchange diffchange-inline">650px</ins>|thumb|center|<ins class="diffchange diffchange-inline">IPTG induction raw data</ins>]]</div></div></td></tr>
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</table>Mamuthttp://2008.igem.org/wiki/index.php?title=Team:Harvard/Parts/LacI&diff=101992&oldid=prevMamut: /* LacI Inducible System */2008-10-30T02:43:52Z<p><span class="autocomment">LacI Inducible System</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="text-indent:0pt;color:black">[[Image:BBa_K098984.png|thumb|650px|center|BBa_K098984 with BioBrick Prefix and Suffix]]</div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="text-indent:0pt;color:black">[[Image:BBa_K098984.png|thumb|650px|center|BBa_K098984 with BioBrick Prefix and Suffix]]</div></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>==Induction Test for <del class="diffchange diffchange-inline">lacI </del>System with GFP==</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>==Induction Test for <ins class="diffchange diffchange-inline">LacI </ins>System with GFP==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In preparing to make the lacI system for mtrB, we tested the IPTG inducibility of [http://partsregistry.org/wiki/index.php?title=Part:BBa_K098982 BBa_K098982] in ''E. coli''. In this system, the repressor (LacI) is driven by a weak promoter.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In preparing to make the lacI system for mtrB, we tested the IPTG inducibility of [http://partsregistry.org/wiki/index.php?title=Part:BBa_K098982 BBa_K098982] in ''E. coli''. In this system, the repressor (LacI) is driven by a weak promoter.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Method===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Method===</div></td></tr>
</table>Mamut