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Team:Harvard/Parts/Other
From 2008.igem.org
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| - | + | ==TetR-based Inducible System== | |
The TetR system we hoped to develop would have been similar to the lacI system. The pTet promoter which controls mtrB expression is regulated by the repressor TetR ([http://partsregistry.org/wiki/index.php?title=Part:BBa_C0040| BBa_C0040]). Expression of mtrB would have occurred when the system is induced with anhydrotetracycline which binds TetR. Unfortunately, as mtrB is toxic, we could not clone successful clone this system. | The TetR system we hoped to develop would have been similar to the lacI system. The pTet promoter which controls mtrB expression is regulated by the repressor TetR ([http://partsregistry.org/wiki/index.php?title=Part:BBa_C0040| BBa_C0040]). Expression of mtrB would have occurred when the system is induced with anhydrotetracycline which binds TetR. Unfortunately, as mtrB is toxic, we could not clone successful clone this system. | ||
| - | However, we did submit parts | + | However, we did submit parts [http://partsregistry.org/wiki/index.php?title=Part:BBa_K098980 BBa_K098980] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_K098981 BBa_K098981] in case you might find them useful. |
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Revision as of 01:32, 30 October 2008
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