Team:Harvard/Parts/Other

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The Tet system we developed is similar to the Lac system. The pTet promoter which controls mtrB expression is regulated by the repressor TetR ([http://partsregistry.org/wiki/index.php?title=Part:BBa_C0040| BBa_C0040]). Expression of mtrB occurs when the system is induced with anhydrotetracycline which binds TetR.
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The TetR system we hoped to develop would have been similar to the lacI system. The pTet promoter which controls mtrB expression is regulated by the repressor TetR ([http://partsregistry.org/wiki/index.php?title=Part:BBa_C0040| BBa_C0040]). Expression of mtrB would have occurred when the system is induced with anhydrotetracycline which binds TetR. Unfortunately, as mtrB is toxic, we could not clone successful clone this system.
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However, we did submit parts [[http://partsregistry.org/wiki/index.php?title=Part:BBa_K098980 BBa_K098980]] and [[http://partsregistry.org/wiki/index.php?title=Part:BBa_K098981 BBa_K098981]] in case you might find them useful.
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Revision as of 01:32, 30 October 2008



Other

The TetR system we hoped to develop would have been similar to the lacI system. The pTet promoter which controls mtrB expression is regulated by the repressor TetR (BBa_C0040). Expression of mtrB would have occurred when the system is induced with anhydrotetracycline which binds TetR. Unfortunately, as mtrB is toxic, we could not clone successful clone this system.

However, we did submit parts [BBa_K098980] and [BBa_K098981] in case you might find them useful.