Team:Harvard/Recipes

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(New page: =Recipes= ==Orianna's growth media== * 50 mM K-PIPES '''18.925 g/L''' * 7.5 mM NaOH '''7.5 mL/L''' * 28 mM NH<sub>4</sub>Cl '''1.5 g/L''' * 1.3 mM KCl ...)
(Lactate solution)
 
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<html><a href = "http://2008.igem.org/Team:Harvard"><img src="http://2008.igem.org/wiki/images/b/b9/Harvard_logo.png"></a></html>
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=Recipes=
=Recipes=
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We experimented with several types of growth media for Shewanella growth, both aerobically and anaerobically. The most effective growth media recipes are listed as follows.<BR><BR>
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==Orianna's growth media==
==Orianna's growth media==
* 50 mM K-PIPES              '''18.925 g/L'''
* 50 mM K-PIPES              '''18.925 g/L'''
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* 5.844 g/L 100mM NaCl
* 5.844 g/L 100mM NaCl
''7.0 pH''
''7.0 pH''
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==Lactate solution==
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* 13.51 g/L Lactic acid
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* neutralize to pH 7 with NaOH
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Latest revision as of 04:08, 30 October 2008



Recipes

We experimented with several types of growth media for Shewanella growth, both aerobically and anaerobically. The most effective growth media recipes are listed as follows.

Contents

Orianna's growth media

  • 50 mM K-PIPES 18.925 g/L
  • 7.5 mM NaOH 7.5 mL/L
  • 28 mM NH4Cl 1.5 g/L
  • 1.3 mM KCl 433 uL/L
  • 4.3 mM NaH2PO4 0.59 g/L
  • 100 mM NaCl 5.84 g/L
  • 18 mM Lactate 1.62 g/L
  • 10 mL Vitamin solution
  • 10 mL Amino acid solution
  • 10 mL Trace mineral solution

LML medium (for anaerobic Shewanella growth)

  • 0.02% yeast extract
  • 0.01% peptone
  • 20 mM lactate
  • 10 mM HEPES [pH 7.4]

LML agar (for Shewanella reduction test)

  • LML medium
  • 0.8% agar
  • 2 mM MnO2- Mn(IV) oxide (turns from brown to clear upon reduction to Mn(II))

(LML recipes from Beliaev and Saffarini 1998)

Chamber media

  • 5.844 g/L 100mM NaCl
  • 15.1185 g/L 50mM PIPES (hydrogen)

7.0 pH

Phosphate buffer

  • 2.918 g/L Monosodium phosphate, monohydrate
  • 4.095 g/L Disodium phosphate, anhydrous
  • 5.844 g/L 100mM NaCl

7.0 pH

Lactate solution

  • 13.51 g/L Lactic acid
  • neutralize to pH 7 with NaOH