"
Page
Discussion
View source
History
teams
Log in
Team:Harvard/Recipes
From 2008.igem.org
Revision as of 22:25, 28 October 2008 by
Joyy
(
Talk
|
contribs
)
(
diff
)
← Older revision
|
Latest revision
(
diff
) |
Newer revision →
(
diff
)
Home
Team
Project
Overview
Organism
Hardware >>
Fuel Cell Development
Experimental Procedures
References
Parts Made
Overview
lacI System
Temperature
sensitive cI
Other
Notebook
Daily Notebook
General Protocols
Recipes
Plasmids
Contents
1
Recipes
1.1
Orianna's growth media
1.2
LML medium (for anaerobic Shewanella growth)
1.3
LML agar (for Shewanella reduction test)
1.4
Chamber media
1.5
Phosphate buffer
Recipes
Orianna's growth media
50 mM K-PIPES
18.925 g/L
7.5 mM NaOH
7.5 mL/L
28 mM NH
4
Cl
1.5 g/L
1.3 mM KCl
433 uL/L
4.3 mM NaH
2
PO
4
0.59 g/L
100 mM NaCl
5.84 g/L
18 mM Lactate
1.62 g/L
10 mL Vitamin solution
10 mL Amino acid solution
10 mL Trace mineral solution
LML medium (for anaerobic Shewanella growth)
0.02% yeast extract
0.01% peptone
20 mM lactate
10 mM HEPES [pH 7.4]
LML agar (for Shewanella reduction test)
LML medium
0.8% agar
2 mM MnO2- Mn(IV) oxide (turns from brown to clear upon reduction to Mn(II))
(LML recipes from
Beliaev and Saffarini 1998
)
Chamber media
5.844 g/L 100mM NaCl
15.1185 g/L 50mM PIPES (hydrogen)
7.0 pH
Phosphate buffer
2.918 g/L Monosodium phosphate, monohydrate
4.095 g/L Disodium phosphate, anhydrous
5.844 g/L 100mM NaCl
7.0 pH