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Team:Harvard/Recipes - Revision history
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Revision history for this page on the wiki
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Linchinlee: /* Lactate solution */
2008-10-30T04:08:59Z
<p><span class="autocomment">Lactate solution</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 04:08, 30 October 2008</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Lactate solution==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Lactate solution==</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* 13.51 g/L <del class="diffchange diffchange-inline">lactic </del>acid</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* 13.51 g/L <ins class="diffchange diffchange-inline">Lactic </ins>acid</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* neutralize to pH 7 with NaOH</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* neutralize to pH 7 with NaOH</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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Linchinlee
http://2008.igem.org/wiki/index.php?title=Team:Harvard/Recipes&diff=104458&oldid=prev
Linchinlee: /* Recipes */
2008-10-30T04:08:23Z
<p><span class="autocomment">Recipes</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* 5.844 g/L 100mM NaCl</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* 5.844 g/L 100mM NaCl</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''7.0 pH''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''7.0 pH''</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">==Lactate solution==</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* 13.51 g/L lactic acid</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* neutralize to pH 7 with NaOH</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td></tr>
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Linchinlee
http://2008.igem.org/wiki/index.php?title=Team:Harvard/Recipes&diff=82831&oldid=prev
Joyy: /* Recipes */
2008-10-28T22:27:13Z
<p><span class="autocomment">Recipes</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Recipes=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Recipes=</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We experimented with several types of growth media for Shewanella growth, both aerobically and anaerobically. The most effective growth media recipes are listed as follows.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We experimented with several types of growth media for Shewanella growth, both aerobically and anaerobically. The most effective growth media recipes are listed as follows.<ins class="diffchange diffchange-inline"><BR><BR></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>__TOC__</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>__TOC__</div></td></tr>
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Joyy
http://2008.igem.org/wiki/index.php?title=Team:Harvard/Recipes&diff=82829&oldid=prev
Joyy: /* Recipes */
2008-10-28T22:27:01Z
<p><span class="autocomment">Recipes</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Recipes=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Recipes=</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">We experimented with several types of growth media for Shewanella growth, both aerobically and anaerobically. The most effective growth media recipes are listed as follows.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>__TOC__</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>__TOC__</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Orianna's growth media==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Orianna's growth media==</div></td></tr>
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Joyy
http://2008.igem.org/wiki/index.php?title=Team:Harvard/Recipes&diff=82822&oldid=prev
Joyy at 22:25, 28 October 2008
2008-10-28T22:25:35Z
<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Recipes=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Recipes=</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">__TOC__</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Orianna's growth media==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Orianna's growth media==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* 50 mM K-PIPES '''18.925 g/L'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* 50 mM K-PIPES '''18.925 g/L'''</div></td></tr>
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Joyy
http://2008.igem.org/wiki/index.php?title=Team:Harvard/Recipes&diff=82818&oldid=prev
Joyy at 22:25, 28 October 2008
2008-10-28T22:25:11Z
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> color:#333333;</ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> width: 12em;</ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">.firstHeading {</ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">#bodyContent {</ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">#content {</ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">p {</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> color:#333333;</ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">body {</ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">.firstHeading {</ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><html><a href = "https://2008.igem.org/Team:Harvard"><img src="https://static.igem.org/mediawiki/2008/b/b9/Harvard_logo.png"></a></html></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Recipes=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Recipes=</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* 5.844 g/L 100mM NaCl</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* 5.844 g/L 100mM NaCl</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''7.0 pH''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''7.0 pH''</div></td></tr>
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</table>
Joyy
http://2008.igem.org/wiki/index.php?title=Team:Harvard/Recipes&diff=69250&oldid=prev
Joyy: New page: =Recipes= ==Orianna's growth media== * 50 mM K-PIPES '''18.925 g/L''' * 7.5 mM NaOH '''7.5 mL/L''' * 28 mM NH<sub>4</sub>Cl '''1.5 g/L''' * 1.3 mM KCl ...
2008-10-26T19:35:32Z
<p>New page: =Recipes= ==Orianna's growth media== * 50 mM K-PIPES '''18.925 g/L''' * 7.5 mM NaOH '''7.5 mL/L''' * 28 mM NH<sub>4</sub>Cl '''1.5 g/L''' * 1.3 mM KCl ...</p>
<p><b>New page</b></p><div>=Recipes=<br />
<br />
==Orianna's growth media==<br />
* 50 mM K-PIPES '''18.925 g/L'''<br />
* 7.5 mM NaOH '''7.5 mL/L'''<br />
* 28 mM NH<sub>4</sub>Cl '''1.5 g/L'''<br />
* 1.3 mM KCl '''433 uL/L'''<br />
* 4.3 mM NaH<sub>2</sub>PO<sub>4</sub> '''0.59 g/L'''<br />
* 100 mM NaCl '''5.84 g/L'''<br />
* 18 mM Lactate '''1.62 g/L'''<br />
* 10 mL Vitamin solution<br />
* 10 mL Amino acid solution<br />
* 10 mL Trace mineral solution<br />
<br />
==LML medium (for anaerobic Shewanella growth)==<br />
* 0.02% yeast extract<br />
* 0.01% peptone<br />
* 20 mM lactate<br />
* 10 mM HEPES [pH 7.4]<br />
<br />
==LML agar (for Shewanella reduction test)==<br />
* LML medium<br />
* 0.8% agar<br />
* 2 mM MnO2- Mn(IV) oxide (turns from brown to clear upon reduction to Mn(II))<br />
<br />
(LML recipes from [http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9829939| Beliaev and Saffarini 1998])<br />
<br />
==Chamber media==<br />
* 5.844 g/L 100mM NaCl<br />
* 15.1185 g/L 50mM PIPES (hydrogen) <br />
''7.0 pH''<br />
<br />
==Phosphate buffer==<br />
* 2.918 g/L Monosodium phosphate, monohydrate<br />
* 4.095 g/L Disodium phosphate, anhydrous <br />
* 5.844 g/L 100mM NaCl<br />
''7.0 pH''</div>
Joyy