Team:Hawaii

From 2008.igem.org

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Latest revision as of 03:55, 30 October 2008

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The Project

Cyanobacteria are photosynthetic bacteria that can convert carbon dioxide to sugars. The main goal of the Hawaii team is to make a "toolkit" for cyanobacteria, which hold promise as producers of biofuels and other bioproducts.

We constructed a number of tools to more easily and effectively engineer cyanobacteria, including

1) a BioBrick switch that turns on a downstream gene when nitrate is added,

2) a BioBrick that mediates protein export from the cell into the surrounding medium and

3) a naturally occurring shuttle vector to BioBrick format to allow easy transfer of existing and new BioBricks into cyanobacteria.

Our toolkit was designed to allow controlled production and recovery of bioproducts. Additionally, our vector has the potential to transform other gram negative bacteria and possibly even plants via Agrobacterium.

Parts	Help	Links/Resources
Parts Search Parts Kit Content (Spring 2008) Parts Kit Instructions Parts We Submitted	BioBrick Help Wiki Help	Protocols iGEM 2008 Homepage Parts Registry Homepage

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 {{Team:Hawaii/Header}}
+[[Image:cyanoblob.jpg|left|130px|]]
+==The Project==
+:Cyanobacteria are photosynthetic bacteria that can convert carbon dioxide to sugars.  The main goal of the Hawaii team is to make a "toolkit" for cyanobacteria, which hold promise as producers of biofuels and other bioproducts.
+:We constructed a number of tools to more easily and effectively engineer cyanobacteria, including
+::::1) a BioBrick switch that turns on a downstream gene when nitrate is added,
+::::2) a BioBrick that mediates protein export from the cell into the surrounding medium and
+::::3) a naturally occurring shuttle vector to BioBrick format to allow easy transfer of existing and new BioBricks into cyanobacteria.
+:Our toolkit was designed to allow controlled production and recovery of bioproducts. Additionally, our vector has the potential to transform other gram negative bacteria and possibly even plants via ''Agrobacterium''.
+----
 <!-- ##<content>## -->
-{|
-|style="background-color:WhiteSmoke;border-width: 0px;padding: 3px;text-align:left;"|Our team aims to create the framework and tools for expanding the scope of BioBrick synthetic biology to organisms other than E. coli.  Creation of a mobilizable broad-host-range plasmid enables the transfer of modularized and abstracted genetic components to act in wide range of organisms that share a similar pool of intermediate metabolites.  A broad-host-range mobilizable vector would serve as a channel to "open source" the rich diversity of genetic information between organisms in a laboratory setting.  We envision a wide diversity of future broad-host-range BioBrick parts, created and transported, with this or similar BioBrick vectors; each designated to be compatible with specific organism(s). These modules can also be combinatorially transformed or combined into organisms as larger devices to produce desired products. [[Team:Hawaii/Project|more...]]
-|}
-<br><br>
 {|class=wikitable align=center width=900
-!<big>Resources</big>
 !<big>Parts</big>
 !<big>Help</big>
-!<big>Links</big>
+!<big>Links/Resources</big>
 |-
-|align=center|
-*[http://packrat.stjohn.hawaii.edu/prestinglab/wiki/iGEM:2008/References References]
-*[https://2008.igem.org/Team:Hawaii/Protocols Protocols]
-*[http://packrat.stjohn.hawaii.edu/prestinglab/wiki/Inventory Inventory]
-*[http://packrat.stjohn.hawaii.edu/prestinglab/wiki/Shopping_List Ordering]
 | align=center|
 *[http://partsregistry.org/cgi/partsdb/search.cgi Parts Search]
@@ Line 32: / Line 35: @@
 *[[Team:Hawaii/Wiki Help|Wiki Help]]
 | align=center|
-*[http://packrat.stjohn.hawaii.edu/prestinglab/wiki/iGEM:2008 Hawaii Team Private Homepage]
+*[https://2008.igem.org/Team:Hawaii/Protocols Protocols]
 *[https://2008.igem.org iGEM 2008 Homepage]
 *[http://partsregistry.org Parts Registry Homepage]
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