Team:Hawaii/Notebook/2008-05-24

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(Difference between revisions)

Revision as of 02:48, 27 May 2008

Margaret

Made seed stocks

five 10mL seed stocks of PCC6803 in BG-11 media
five plates smeared with 200uL PCC6803 in BG-11 media, all from old plates

Hypothesis: Liquid Cultures of PCC6803 clump when incubated in Erlenmeyer Flasks and does not when cultured in "box" culture flasks: Is this due to the motion of the media in different containers, the surface area of the culture, is it reproducible?

Method 1: Does the clump go back to homogeneous?
1. Took 0.5mL of a clump and placed it in 2 culture bottles, 1 of the bottles was fresh BG-11 media, the other was the old stock to which buffer was added today.
2. Took 0.5mL of a homogeneous liquid culture and placed it in 2 culture bottles, 1 of the bottles was fresh BG-11 media, the other 1 was the old stock to which buffer was added today.
Method 2:Will previously homogeneous culture go to clump?
1. Homogeneous liquid culture placed in BG-11 media in an Erlenmeyer flask 1:10, Vfinal=50 mL
2. Homogeneous liquid culture placed in BG-11 media in an Culture flask 1:10, Vfinal=50 mL

Discussion

What to do in the future:
1. The plates are drying out too soon so we need to make them 5mm thick instead of 3mm thick.
2. Get autoclave certification. We need to make media and autoclave hood materials: paper towels (?), eppendorf tubes, blocks, etc.
3. Get Pictures!

If our action is not recorded, we are doomed to either repeat the error, or fail to replicate the success.

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

@@ Line 1: / Line 1: @@
 == Margaret ==
+'''Made seed stocks'''
-#Made some seed stocks
+#five 10mL seed stocks of PCC6803 in BG-11 media
-##5 10mL seed stocks of PCC6803 in BG-11 media
+#five plates smeared with 200uL PCC6803 in BG-11 media, all from old plates
-##5 plates smeared with 200uL PCC6803 in BG-11 media, all from old plates
+'''Hypothesis:''' Liquid Cultures of PCC6803 clump when incubated in Erlenmeyer Flasks and does not when cultured in "box" culture flasks: Is this due to the motion of the media in different containers, the surface area of the culture, is it reproducible?
-#Hypothesis 1: Liquid Cultures of PCC6803 clump when incubated in Erlenmeyer Flasks, is this an artifact from the Erlenmeyer flask, the difference in surface area, or the volume of the media:
+#Method 1: Does the clump go back to homogeneous?
-##Method 1: Does the clump go back to homogeneous?
+##Took 0.5mL of a clump and placed it in 2 culture bottles, 1 of the bottles was fresh BG-11 media, the other was the old stock to which buffer was added today.
-###Took 0.5mL of a clump and placed it in 2 culture bottles, 1 of the bottles were fresh BG-11 media, the other 1 was of the old stock to which buffer was added today.
+##Took 0.5mL of a homogeneous liquid culture and placed it in 2 culture bottles, 1 of the bottles was fresh BG-11 media, the other 1 was the old stock to which buffer was added today.
-###Took 0.5mL of a homogeneous liquid culture and placed it in 2 culture bottles, 1 of the bottles were fresh BG-11 media, the other 1 was of the old stock to which buffer was added today.
+#Method 2:Will previously homogeneous culture go to clump?
-##Method 2:Will previously homogeneous culture go to clump?
+##Homogeneous liquid culture placed in BG-11 media in an Erlenmeyer flask 1:10, Vfinal=50 mL
-###Homogeneous liquid culture placed in BG-11 media in an Erlenmeyer flask 1:10, Vfinal=50 mL
+##Homogeneous liquid culture placed in BG-11 media in an Culture flask 1:10, Vfinal=50 mL
-###Homogeneous liquid culture placed in BG-11 media in an Culture flask 1:10, Vfinal=50 mL
+[[Image:declumping_test_detail1.jpg]]
+[[Image:declumping_detail_clump.jpg]]
+[[Image:homogeneous_to_clump_50mL.jpg]]
 == Discussion ==