Team:Hawaii/Notebook/2008-10-13
From 2008.igem.org
Projects | Events | Resources | ||
---|---|---|---|---|
Sponsors | Experiments | Milestones | Protocols | |
Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Verification of transformants
- Grace
- Colony PCR of restreak plates
- Bands ~600bp. Plasmid did not cut.
- Krystle
- RE digest of plasmid w/ EcoRI and PstI
- RE digest of prpgt, rgt1 plasmids with EcoRI and PstI
- Ran RE digests on gel
- Bands did not correspond to what was supposedly loaded
- Grace
- PCR of prpgt #5, 7, 11, nrsg #6, rgt #1
- Overnight RE digest of devices with EcoRI and PstI and rgt#1 with XbaI and PstI
Triparental Conjugation
- Grace
- Restreaked 5%LB+BG-11 plates onto BG-11+sp2.5+sm2.5 plates for isolation of cyanos
- Inoculated BG-11+sp2.5+sm2.5 liquid media with conjugated E. coli/cyanos
- Incubated at 37C with 2% CO2 for 11 days. Liquid cultures were shaken at 100 rpm also.
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson