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Team:Heidelberg/Notebook/Killing I/Notebook/week12
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'''Week 12''' | '''Week 12''' | ||
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| + | ==Monday, 10/20/08 == | ||
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| + | ===Proceedings of phage cloning strategy one=== | ||
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| + | * the fully mutated insert in pBluescript SK II was digested with XbaI/XhoI and the insert extracted from the gel | ||
| + | * lambda DNA was also cut with XbaI/XhoI and the backbone also extracted from the gel. | ||
| + | * the insert was ligated in the lambda backbone over night using standard ligation methods | ||
| + | |||
[[Team:Heidelberg/Notebook/Killing_I/Notebook|Back to the overview]] | [[Team:Heidelberg/Notebook/Killing_I/Notebook|Back to the overview]] | ||
Revision as of 10:12, 29 October 2008
Week 12
Monday, 10/20/08
Proceedings of phage cloning strategy one
- the fully mutated insert in pBluescript SK II was digested with XbaI/XhoI and the insert extracted from the gel
- lambda DNA was also cut with XbaI/XhoI and the backbone also extracted from the gel.
- the insert was ligated in the lambda backbone over night using standard ligation methods
