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Team:Illinois/Protocols
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The [http://en.wikipedia.org/wiki/PCR wikipedia page on PCR] is good; I would skip the sections on 'variants' and history. Also their page on [http://en.wikipedia.org/wiki/Molecular_cloning cloning genes into cells] is good, and [http://en.wikipedia.org/wiki/Subcloning subcloning], all of which we'll probably end up using. And [http://en.wikipedia.org/wiki/Bacterial_artificial_chromosome BACs] and [http://en.wikipedia.org/wiki/Yeast_artificial_chromosome YACs]. | The [http://en.wikipedia.org/wiki/PCR wikipedia page on PCR] is good; I would skip the sections on 'variants' and history. Also their page on [http://en.wikipedia.org/wiki/Molecular_cloning cloning genes into cells] is good, and [http://en.wikipedia.org/wiki/Subcloning subcloning], all of which we'll probably end up using. And [http://en.wikipedia.org/wiki/Bacterial_artificial_chromosome BACs] and [http://en.wikipedia.org/wiki/Yeast_artificial_chromosome YACs]. | ||
| - | [http://www.sumanasinc.com/webcontent/animations/content/pcr.html A good animation on basic PCR] | + | [http://www.sumanasinc.com/webcontent/animations/content/pcr.html A good animation on basic PCR]; and a good animation on [http://www.youtube.com/watch?v=acKWdNj936o plasmid methods]. |
Decent explanation and some examples of [http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/R/RecombinantDNA.html Recombinant DNA techniques]. | Decent explanation and some examples of [http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/R/RecombinantDNA.html Recombinant DNA techniques]. | ||
Revision as of 02:20, 10 June 2008
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Contents |
Current Tasks and Subgroup Status
Polymerase Chain Reaction Info and Protocols
PCR is the foundation for everything we will do ever. Below are some links on the basic principles and practice of pcr; as we begin our own protocols those specific steps will be recorded here.
I would start here for a nice intro to the historical context of PCR.
The wikipedia page on PCR is good; I would skip the sections on 'variants' and history. Also their page on cloning genes into cells is good, and subcloning, all of which we'll probably end up using. And BACs and YACs.
A good animation on basic PCR; and a good animation on plasmid methods.
Decent explanation and some examples of Recombinant DNA techniques.
Something trippy.
Yeast Culture
Longterm storage and streaking protocols
Growth and manipulation of yeast
Yeast transformation protocols
Methods in Yeast Genetics: A Cold Spring Harbor Laboratory Course Manual (1998 edition available in Biology library, newer editions currently checked out)
Yeast Info on OWW Contains General, Protocols, Materials, and References Info - Alzar
Directed Evolution
Malaria
The malaria group has found that malaria would be a poor target as the protazoan is never found in the water during its lifecycle. Our new target is typhoid or cholera.
E. coli
check e.coli research articles
Opimization of recombitant E. coli protein expression in growth medium
E. coli gene expression protocols (book available online on google books)
General indications and protocols for the expression in bacteria
Glycerol storage of E. coli bacterial stocks
Different liquid culture media for E. coli
Different dry culture media for bacterial growth
Detection of water-borne E. coli O157 using the integrating waveguide biosensor
GPCRs
Yeast System as a Screening Tool for Pharmacological Assessment of G Protein Coupled Receptors
Typhoid Biology
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