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Team:Illinois/Protocols
From 2008.igem.org
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Contents |
Current Tasks and Subgroup Status
Yeast Culture
Longterm storage and streaking protocols
Growth and manipulation of yeast
Yeast transformation protocols
Methods in Yeast Genetics: A Cold Spring Harbor Laboratory Course Manual (1998 edition available in Biology library, newer editions currently checked out)
Yeast Info on OWW Contains General, Protocols, Materials, and References Info - Alzar
Directed Evolution
Malaria
E. coli
check e.coli research articles
Frozen Competent E.Coli Cells. Methods and protocol for freezing E.Coli Bacteria Competent Cells in aliquots for later use re-use. http://www.ciwemb.edu/labs/koshland/Protocols/BACTERIA/ecolicells.ht
Opimization of recombitant E. coli protein expression in growth medium
http://minds.wisconsin.edu/handle/1793/7956
E. coli gene expression protocols (book available online on google books) http://books.google.com/books?id=TH7OJGY7Br8C&pg=PA1&dq=protein+expression+e+coli&as_brr=0&sig=S18cODgXA7zmQFbsVcrOBwPVKRU#PPA92,M1
General indications and protocols for the expression in bacteria http://www.pepcore.embl.de/protein_expression/ecoli/index.html
Glycerol Storage of E.coli bacterial stocks http://forums.biotechinstitute.org/showthread.php?t=60
Different liquid culture media for E. coli http://www.teknova.com/liquids/liquid-tbs.shtml
Different dry culture media for bacterial growth http://www.teknova.com/dry_media/dry-lbagars.shtml
GPCRs
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