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Team:Illinois/Protocols
From 2008.igem.org
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Current Tasks and Subgroup Status
Polymerase Chain Reaction Protocols
PCR is the foundation for everything we will do ever so it's important to understand what it is. Below are some links on the basic principles and practice of pcr; as we begin our own protocols their specific steps will be recorded here.
I would start here for a nice intro to the basic applications of PCR.
The wikipedia page on PCR is good; I would skip the sections on 'variants' and history. Also their page on cloning genes into cells is good, and subcloning, all of which we'll probably end up using.
Yeast Culture
Longterm storage and streaking protocols
Growth and manipulation of yeast
Yeast transformation protocols
Methods in Yeast Genetics: A Cold Spring Harbor Laboratory Course Manual (1998 edition available in Biology library, newer editions currently checked out)
Yeast Info on OWW Contains General, Protocols, Materials, and References Info - Alzar
Directed Evolution
Malaria
The malaria group has found that malaria would be a poor target as the protazoan is never found in the water during its lifecycle. Our new target is typhoid or cholera.
E. coli
check e.coli research articles
Opimization of recombitant E. coli protein expression in growth medium
E. coli gene expression protocols (book available online on google books)
General indications and protocols for the expression in bacteria
Glycerol storage of E. coli bacterial stocks
Different liquid culture media for E. coli
Different dry culture media for bacterial growth
Detection of water-borne E. coli O157 using the integrating waveguide biosensor
GPCRs
Yeast System as a Screening Tool for Pharmacological Assessment of G Protein Coupled Receptors
Typhoid Biology
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