Team:Imperial College/Growth
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*Grow the plates at 37°C overnight | *Grow the plates at 37°C overnight | ||
*The following day count the number of colonies on the plates and multiply up by the dilution to obtain the colony forming units at each time point. Remember when plating the cells only 100ul of 1000ul was used to plate and so you need to multiply by a further 10. | *The following day count the number of colonies on the plates and multiply up by the dilution to obtain the colony forming units at each time point. Remember when plating the cells only 100ul of 1000ul was used to plate and so you need to multiply by a further 10. | ||
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+ | {{Imperial/EndPage|Protocols|}} |
Revision as of 18:20, 26 October 2008
Cell Count v Optical Density Curve CalibrationAimTo produce a calibration curve to aid in the normalising of fluorescence values to allow proper characterisation of Promoters and RBSs for B.subitlis. This protocol must give results that are as accurate as possible over a considerable range of Optical Densities. Equipment
Reagents
Protocol
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