Team:Imperial College/Light
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======Nutrient Response====== | ======Nutrient Response====== | ||
- | RsbP is the node for nutrient stress response. In conditions of low ATP and GTP RsbP. It is not known exactly how the RsbP responds to the signals, RsbP contains a PAS motif that in some regulatory systems can respond directly to changes in electron transport, proton motive force, or redox potential, changes that typically precede shifts in high-energy nucleotide levels | + | RsbP is the node for nutrient stress response. In conditions of low ATP and GTP RsbP. It is not known exactly how the RsbP responds to the signals, RsbP contains a PAS motif that in some regulatory systems can respond directly to changes in electron transport, proton motive force, or redox potential, changes that typically precede shifts in high-energy nucleotide levels ({{Ref|7}}). |
======Environmental Response====== | ======Environmental Response====== | ||
- | The physical stress phosphatase (RsbU) requires a second protein (RsbT) for activity. Stress is thought to initiate a process that triggers the release of RsbT from a large inhibitory complex composed of multiple copies of two protein species, RsbR (and/or its paralogues) and RsbS. The stress-derived signal driving RsbT release is unknown, but it fails to develop in ''B. subtilis'' lacking either ribosome protein L11 or the ribosome-associated protein Obg. RsbR, RsbS, RsbT, Obg and ribosomes elute in common high-molecular-mass fractions during gel-filtration chromatography of crude ''B. subtilis'' extracts | + | The physical stress phosphatase (RsbU) requires a second protein (RsbT) for activity. Stress is thought to initiate a process that triggers the release of RsbT from a large inhibitory complex composed of multiple copies of two protein species, RsbR (and/or its paralogues) and RsbS. The stress-derived signal driving RsbT release is unknown, but it fails to develop in ''B. subtilis'' lacking either ribosome protein L11 or the ribosome-associated protein Obg. RsbR, RsbS, RsbT, Obg and ribosomes elute in common high-molecular-mass fractions during gel-filtration chromatography of crude ''B. subtilis'' extracts ({{Ref|8}}). |
Go back to the <html><a href="https://2008.igem.org/Team:Imperial_College/Chassis_1"></html>'''>>> Specifications Page >>>'''<html></a></html>|[[Image:Stress response.gif|right|thumb|250px| Image from the <html><a href="http://biop.ox.ac.uk/www/lj2002/rl/fig6_1.html" target="_blank">Lab of Molecular Biophysics</a></html> Oxford University ]] | Go back to the <html><a href="https://2008.igem.org/Team:Imperial_College/Chassis_1"></html>'''>>> Specifications Page >>>'''<html></a></html>|[[Image:Stress response.gif|right|thumb|250px| Image from the <html><a href="http://biop.ox.ac.uk/www/lj2002/rl/fig6_1.html" target="_blank">Lab of Molecular Biophysics</a></html> Oxford University ]] | ||
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{{Imperial/Box2|| | {{Imperial/Box2|| | ||
=====References===== | =====References===== | ||
- | # New Insights into Metabolic Properties of Marine Bacteria Encoding Proteorhodopsins ( | + | # {{FormatRef|||New Insights into Metabolic Properties of Marine Bacteria Encoding Proteorhodopsins||||}} ([http://biology.plosjournals.org/archive/1545-7885/3/8/pdf/10.1371_journal.pbio.0030273-p-L.pdf PDF]) |
- | # Light powered E. | + | # {{FormatRef|||Light powered E. coli with proteorhodopsin||||}} ([http://www.pubmedcentral.nih.gov/picrender.fcgi?artid1892948&blobtype{{Equals}}pdf PDF]) |
- | # Blue Light Activates the σB -Dependent Stress Response of ''Bacillus subtilis'' via YtvA | + | # {{FormatRef|vila-Pérez, M.A.; Hellingwerf, K.J. & Kort, R.||Blue Light Activates the σB -Dependent Stress Response of ''Bacillus subtilis'' via YtvA||||}} [http://jb.asm.org/cgi/content/abstract/188/17/6411] |
- | # First Evidence for Phototropin-Related Blue-Light Receptors in Prokaryotes. | + | # {{FormatRef|Losi, A.; Polverini, E.; Quest, B. & Gärtner, W.||First Evidence for Phototropin-Related Blue-Light Receptors in Prokaryotes.||||}} [http://www.biophysj.org/cgi/content/abstract/82/5/2627] |
- | # Listening to the blue: the time-resolved thermodynamics of the bacterial blue-light receptor YtvA and its isolated LOV domain | + | # {{FormatRef|Losi, A.; Quest, B. & Gärtner, W.||Listening to the blue: the time-resolved thermodynamics of the bacterial blue-light receptor YtvA and its isolated LOV domain||||}} [http://www.rsc.org/Publishing/Journals/PP/article.asp?doi{{Equals}}b301782f] |
- | # Photosensing in chemotrophic, non-phototrophic bacteria: | + | # {{FormatRef|van der Horst, M.A.; Key, J. & Hellingwerf, K.J.||Photosensing in chemotrophic, non-phototrophic bacteria: Let there be light sensing too||||}} ([http://www.sciencedirect.com/science?_ob{{Equals}}MImg&_imagekey{{Equals}}B6TD0-4R5G81C-2-1&_cdi{{Equals}}5184&_user{{Equals}}217827&_orig{{Equals}}search&_coverDate{{Equals}}12%2F31%2F2007&_sk{{Equals}}999849987&view{{Equals}}c&wchp{{Equals}}dGLzVlz-zSkWb&md5{{Equals}}3e1fb96f1e3c38c41b123a2bb89b3878&ie{{Equals}}/sdarticle.pdf PDF]) |
- | + | # {{FormatRef|||||||}} ([http://www.ncbi.nlm.nih.gov/pubmed/16267279 PubMed]) | |
- | # | + | # {{FormatRef|||||||}} ([http://www.ncbi.nlm.nih.gov/pubmed/15583165 PubMed]) |
- | # | + | |
|}} | |}} | ||
{{Imperial/EndPage|Chassis_1|Chassis_1}} | {{Imperial/EndPage|Chassis_1|Chassis_1}} |
Revision as of 16:58, 29 October 2008
Light Sensing
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