Team:Imperial College/XL1 Blue
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(New page: {{Imperial/StartPage2}} ==Preparation of XL1-Blue Electrocompetent cells== ===Aims=== Preparation of ''E. coli'' cells for the cloning of Biobricks and construct construction ===Equipme...) |
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##Using a repeating pipette makes this process much faster and reduces risk of contamination | ##Using a repeating pipette makes this process much faster and reduces risk of contamination | ||
#Store at -70°C | #Store at -70°C | ||
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Revision as of 14:49, 26 October 2008
Preparation of XL1-Blue Electrocompetent cellsAimsPreparation of E. coli cells for the cloning of Biobricks and construct construction Equipment4,000RPM Centrifuge Sterile Centrifugation bottles 50ml Tubes Large Flasks Eppendorf Tubes P200 Pipette Stripettes Reagents1 litre of LB medium Tetracycline 1-2 litres of autoclaved and chilled ddH2O 10% glycerol in ddH2O, autoclaved and chilled Dry ice bath ProtocolKeep Everything Cold where possible - if using a carbon fibre rotor, you may want to put it in a cold room after innoculation. Set aside an afternoon for this, starting the culture in the morning Check the culture while growing frequently
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